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Combined effects of water temperature and copper ion concentration on catalase activity in Crassostrea ariakensis

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Abstract

A central composite experimental design and response surface method were used to investigate the combined effects of water temperature (18–34°C) and copper ion concentration (0.1–1.5 mg/L) on the catalase (CAT) activity in the digestive gland of Crassostrea ariakensis. The results showed that the linear effects of temperature were significant (P<0.01), the quadratic effects of temperature were significant (P<0.05), the linear effects of copper ion concentration were not significant (P>0.05), and the quadratic effects of copper ion concentration were significant (P<0.05). Additionally, the synergistic effects of temperature and copper ion concentration were not significant (P>0.05), and the effect of temperature was greater than that of copper ion concentration. A model equation of CAT enzyme activity in the digestive gland of C. ariakensis toward the two factors of interest was established, with R 2, Adj. R 2 and Pred. R 2 values as high as 0.943 7, 0.887 3 and 0.838 5, respectively. These findings suggested that the goodness of fit to experimental data and predictive capability of the model were satisfactory, and could be practically applied for prediction under the conditions of the study. Overall, the results suggest that the simultaneous variation of temperature and copper ion concentration alters the activity of the antioxidant enzyme CAT by modulating active oxygen species metabolism, which may be utilized as a biomarker to detect the effects of copper pollution.

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Authors and Affiliations

  1. School of Life Sciences of Huaiyin Normal University, Huai’an, 223300, China

    Hui Wang  (王辉)

  2. Fisheries College of Guangdong Ocean University, Zhanjiang, 524088, China

    Hongshuai Yang  (杨洪帅), Jiahui Liu  (刘加慧), Yanhong Li  (李艳红) & Zhigang Liu  (刘志刚)

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  1. Hui Wang  (王辉)
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  2. Hongshuai Yang  (杨洪帅)
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  3. Jiahui Liu  (刘加慧)
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  4. Yanhong Li  (李艳红)
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  5. Zhigang Liu  (刘志刚)
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Correspondence to Zhigang Liu  (刘志刚).

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Supported by the Guangdong Province Science & Technology Project (No. 2010B020201014), the Guangdong Province Education Department (No. GCZX-A0909), the Guangdong Province Ocean and Fisheries Science & Technology Extension Project (No. 20120980), the Guangdong Province Industry-University-Science Partnership Project (No. 20110908), and the Sci & Tech Plan of Huaiyin Normal University (No. 00wh0031)

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Wang, H., Yang, H., Liu, J. et al. Combined effects of water temperature and copper ion concentration on catalase activity in Crassostrea ariakensis . Chin. J. Ocean. Limnol. 33, 905–912 (2015). https://doi.org/10.1007/s00343-015-4212-9

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  • DOI: https://doi.org/10.1007/s00343-015-4212-9

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