Ephemeral hypoxia reduces oxygen consumption in the Caribbean coral Orbicella faveolata

Oxygen concentrations in coastal waters have declined globally by 10% since the mid-twentieth century, and ocean warming will further reduce the solubility of oxygen in coastal habitats. Some nearshore reefs experience periodic hypoxic conditions due to eutrophication, especially during the wet season. Here, we determined the combined impacts of hypoxia and elevated temperature on the reef-building coral, Orbicella faveolata, by exposing corals to normoxic or hypoxic conditions and ambient or elevated temperatures. Oxygen consumption was monitored using closed-system respirometry. Corals within hypoxic conditions consumed 34% less oxygen relative to corals in normoxic conditions. Corals in the elevated temperature normoxic treatment experienced a 10% increase in oxygen consumption relative to the control. Corals exposed to both stressors simultaneously experienced a 62% reduction in oxygen consumption. These results suggest that increased temperature may exacerbate the negative effects of hypoxia on O. faveolata.


Introduction
Coral reefs are some of the most biologically diverse and economically important ecosystems in the world, providing ecosystem services that are vital to coastal communities such as shoreline protection from storm surge, fisheries, and ecotourism (Moberg and Folke 1999;Storlazzi et al. 2019). For example, in the continental U.S., Florida's Coral Reef (FCR) spans 360 nautical miles along the east coast of the state from Martin County to the Dry Tortugas. Economically, the FCR has an asset value of $8.5 billion, supports over 70,000 local jobs, and attracts 16 million visitors per year (Johns et al. 2001). Despite the biological and economic importance of coral reefs, several local and global stressors have drastically reduced live coral cover over the last several decades (Dustan 1977;Palandro et al. 2008;Ruzicka et al. 2013;Toth et al. 2019).
Global stressors such as ocean warming have contributed to a decline in live coral cover on reefs around the world (Brandt and McManus 2009;Ruzicka et al. 2013;Manzello 2015;Manzello et al. 2018;Jones et al. 2020). Additionally, stressors such as pollution, overfishing, and infectious disease outbreaks have led to further coral reef decline (Green and Bruckner 2000;Aronson and Precht 2001;Mora 2008;Jackson et al. 2017;Lapointe et al. 2019). Specifically, the Stony Coral Tissue Loss Disease (SCTLD-a multi-year disease related mortality event) has resulted in massive declines of major reef building species throughout the Caribbean over the last several years (Precht et al. 2016;Walton et al. 2018;Alvarez-Filip et al. 2019;Weil et al. 2019). Furthermore, some locations like Florida reefs, now have less than 5% live coral cover, and show little to no signs of recovery (Toth et al. 2019;Porter and Stossel 2020). In addition to the global and local stressors mentioned, some nearshore coral reefs can  (Lapointe and Matzie 1996;Altieri et al. 2017;Kealoha et al. 2020). For example, Bocas del Toro, Panama, experienced a hypoxic event in 2010 where corals were subjected to oxygen levels of \ 0.5 mg L -1 at some sites (Collin et al. 2009;Altieri et al. 2017). This location then experienced another hypoxic event in 2017 that led to a 50% loss of live coral and benthic community shifts (Johnson et al. 2021). More recently, the Flower Garden Banks in the Gulf of Mexico was reported to experience a severe hypoxic event with dissolved oxygen levels reaching \ 1 mg L -1 in some locations (Kealoha et al. 2020).
These studies further report that the negative effects of oxygen deprivation can lead to bleaching stress and eventual mortality, with some coral species being more susceptible to hypoxia than others. The combination of elevated temperature and lower dissolved oxygen concentrations may pose additional physiological strain for some nearshore reef-building corals. Therefore, our objective was to determine the combined effect of hypoxia and elevated temperature, as singular and simultaneous stressors, on the endangered reef building coral, Oribcella faveolata, in a controlled laboratory setting. We hypothesized that exposure to: (1) elevated temperature would increase oxygen consumption, (2) hypoxia would result in sublethal effects by reducing oxygen consumption, and (3) the combined treatment (elevated temperature and hypoxia) would produce an antagonistic effect on oxygen consumption relative to the control.

Coral collection and fragmentation
Two healthy 10 cm 9 10 cm Orbicella faveolata colonies were collected at an inshore patch reef near Big Pine Key, FL (24.60580, -81.42946) in August 2018 and maintained in the Coral Health and Disease laboratory at Mote Marine Laboratory in Sarasota, FL. These colonies were fragmented into 60 individual fragments (n = 15 per treatment; treatments described below) using a diamond blade band saw (Gryphon Corporation, C-40 CR Aquasaw XL). Fragments were allowed to recover and grow within a flow-through raceway for approximately 10 months prior to the beginning of the experiment. During that time, corals were held in ambient water quality conditions within a narrow range (mean, ± S.D.) of temperature (27.0 ± 0.46°C), salinity (34.2 ± 0.87) and dissolved oxygen (7.78 ± 0.62 mg L -1 ). Corals were maintained under a 10-h light: 14-h dark photoperiod using Radion XR30w Pro aquaria lights (Ecotech Marine, Allentown, Pennsylvania) and were set to a ''traveling sunrise and sunset'' feature (50-300 mol m -2 s -1 photosynthetically active radiation) to mimic natural conditions. Prior to exposure to the treatments, each fragment was digitally photographed and the surface area (cm 2 ) was quantified by digitally tracing the edge of each fragment using ImageJ software (Abràmoff et al. 2004).

Experimental design
This experiment consisted of two fully-crossed treatment parameters (i.e., temperature and dissolved oxygen), each with two levels, resulting in a total of four different treatments. We used 15 independent corals in each treatment (60 total). Caution was taken during experimentation to make sure that corals from one parent colony were not exclusively within one treatment. Corals from parent colony ''A'' had eight individuals in the control and hypoxic treatment and seven individuals in the elevated temperature and combined treatment. Corals from colony ''B'' brought the replicates to 15 in each treatment. The two temperature levels were set at 28°C (ambient) and 31.5°C (elevated). Ambient temperature conditions reflected average ambient conditions for Carysfort Reef, Florida and average summer temperatures (National Data Buoy Center 1971; Kuffner et al. 2015). The elevated temperature was set to mimic thermal conditions that corals might experience during a bleaching event. The oxygen levels consisted of a normoxic treatment ([ 2.0 mg L -1 ) and a hypoxic treatment (see Table 1 for a summary of treatment levels). We defined hypoxic as oxygen concentrations below 2 mg O 2 L -1 , while anoxic conditions were defined as the complete absence of oxygen (Diaz 2001;Breitburg et al. 2018), however, no corals experienced anoxic conditions during these experiments.
Temperature was controlled during the experiment using a thermostatically controlled flow-through water bath. The temperature of the water bath was digitally controlled using heaters and temperature probes, which were constantly monitored and maintained by AquaControllers (Apex System, Neptune). This system allowed corals to be gradually acclimated to the elevated temperature treatment by raising the temperature 0.5°C every day over a period of seven days. Corals in the elevated temperature treatments were then maintained at 31.5°C for seven days prior to the start of the experiment. Corals were gradually exposed to hypoxic conditions over a two-hour period, during which the dissolved oxygen concentrations were reduced by 0.035 mg L -1 per minute for each temperature treatment by bubbling nitrogen gas into the seawater. Corals were dark acclimated for one hour prior to experimentation in a darkroom. After the gradual ramp down to lower dissolved oxygen concentrations, corals were maintained in hypoxic conditions (at their respective temperature treatment) for six hours in the dark, which mimicked nocturnal conditions. The corals were then placed into the respirometry chamber for one hour to measure oxygen consumption.
We acknowledge that the use of nitrogen gas to reduce oxygen concentrations will increase seawater pH, which may be different from natural hypoxic events (Alteri et al. 2017). In our experiments, corals were exposed to hypoxia in dark conditions when only respiration is taking place, which results in a lower pH at the coral surface-water interface than in light conditions (Al-Horani 2005; Jokiel 2011). The magnitude of the surface pH decreases in the dark conditions was likely minimized due to the buffering capacity of injecting nitrogen gas used in these experiments; however, it is unlikely that this difference would have impacted oxygen consumption by the coral.

Coral respirometry
To determine the effects of the experimental treatments on coral physiology, total oxygen consumption (lmol L -1 ) was measured for each coral fragment. Oxygen consumption was continuously monitored over one hour using a microrespirometry chamber and oxygen probe (Unisense; 400 ml chamber). Simultaneously, a second chamber with the same experimental conditions, but no coral was used as a ''blank.'' This protocol allowed us to account for oxygen produced or consumed by unknown biological sources in the treatment seawater. Both chambers were sealed and submerged in a thermally controlled water bath as previously described on a submerged stir plate (400 rpm) to ensure uniform flow. All experiments were conducted in a darkroom, and all seawater used in the respirometer was vacuum filtered (0.1 lm) prior to use to minimize biological sources of respiration and photosynthesis.
Both respirometer chambers were equipped with a stir bar (400 rpm) separated from the experimental chamber via a mesh screen, and were placed on a stir plate to ensure that the water in the chamber was well-mixed. Prior to experimentation, oxygen sensors were two-point calibrated using anoxic seawater bubbled with nitrogen gas (0% oxygen concentration) and saturated seawater bubbled with air (100% oxygen). Both oxygen electrodes relayed realtime measurements within the respirometers every second for 65 min to a computer for data output using data acquisition software (Unisense, AutoResp). We counted the first 5 min of every trial as an additional acclimation period, which were not included in the analyses. We used the total amount of oxygen consumed (lmol L -1 ) after 60 min as the primary metric for evaluating the impact of our treatments on coral respiration.

Data analysis
The total amount of oxygen consumed (lmol L -1 ) after 60 min did not meet the assumptions of normality and were, therefore, rank transformed (Conover and Iman, 1981). A linear mixed effects model was run on the ranked data, with temperature and dissolved oxygen as fixed factors and coral as a random factor. All statistical analyses were performed using R (R Core Team, 2021).

Results and discussion
Coral reefs around the world are increasingly impacted by thermal extremes, which have been responsible for widespread coral bleaching and mortality . For example, both warm and cold extremes have caused declines in reef-building coral populations in south Florida (Lirman et al. 2011;Manzello 2015;Manzello et al. 2015;Jones et al. 2020); however, less is known about the sublethal impacts of both thermal extremes and reduced dissolved oxygen on coral physiology. On average, O. faveolata in the control consumed 6.37 lmol L -1 of oxygen after 60 min in dark conditions, while corals exposed to elevated temperature consumed 7.05 lmol L -1 . The difference in oxygen consumption under normoxic conditions was not statistically different from the control (elevated temperature: F 1,56 = 0.02, P = 0.86; Fig. 1, Table 2). Al-Horani (2005) found that Indo-pacific corals also consumed more oxygen at elevated temperature (32°C); however, the increase in that experiment was observed under light conditions and was associated with a decrease in photosynthesis which was not the case in our experiment that was conducted in dark conditions. Elevated seawater temperatures are known to increase respiration rates and have been reported in other species like Montipora sp. (Coles and Jokiel 1977;Porter et al. 1999). The lack of a significant effect at elevated temperature in these experiments is most likely due to biological variation among the corals and our lower level of replication. Corals in both nearshore and offshore environments, throughout parts of the Caribbean and within the Flower Garden Banks, may be periodically subjected to excess nutrients associated with runoff during the wet season, especially after major storm events (Lapointe et al. 2004;Altieri et al. 2017;Kealoha et al. 2020). These excess nutrients can cause episodic hypoxic events in some habitats (Lipp et al. 2002;Altieri et al. 2017;Kealoha et al. 2020). Here, we show that corals exposed to hypoxic conditions as a singular stressor experienced a significant decrease in the amount of oxygen consumed (dissolved oxygen: F 1,56 = 7.5, P = 0.007; Fig. 1, Table 2). Corals in the hypoxic treatment consumed 4.19 lmol L -1 of oxygen, which was representative of a 34.2% decrease in oxygen consumed relative to corals in the control. Corals in the combined elevated temperature and hypoxic treatment consumed almost two times less oxygen (2.37 lmol L -1 of oxygen), which was representative of a 62.8% decrease relative to the control. We did not observe a significant interaction effect between temperature and the dissolved oxygen treatments (F 1,56 = 0.36, P = 0.55, Table 2).
In the present study, corals were still utilizing aerobic respiration pathways in all treatments, as we observed reductions in oxygen concentrations after 60 min of measurement, albeit less effectively in some treatments. This suggests that O. faveolata may have some tolerance to short-term hypoxic exposure (i.e., diurnal changes). The observed reduction in oxygen consumption during hypoxic stress may, therefore, be associated with a gradual shift to anaerobic respiration in O. faveolata. Experiments with longer-term exposure are therefore needed to assess whether hypoxia could cause a complete shift to anaerobic respiration and to identify what those impacts may be on coral physiology. Some cnidarians have been reported to be able to survive hypoxic and even anoxic conditions for greater than three and five days of exposure, respectively (Martinez et al. 2012;Murphy and Richmond 2016); however, tolerance to reduced oxygen in the short-term comes at a metabolic cost (Martinez et al. 2012;Murphy and Richmond 2016). Cnidarians can tolerate acute hypoxic periods by shifting their metabolism to use less efficient forms of respiration (e.g., anaerobic respiration pathways) and converting pyruvate to lactate or some other enzyme during glycolysis (Murphy and Richmond 2016). These metabolic shifts during hypoxic exposure can often lead to bleaching of the colony (Zhu et al. 2004;Altieri et al. 2017;Nelson and Altieri 2019). Although we did not Fig. 1 The amount of oxygen (lmol L -1 ; ± SE) consumed during 1 h respirometry trials for Orbicella favolata (n = 15 per treatment) exposed to hypoxia and elevated temperature as singular and simultaneous stressors. Letters denote significant differences at a = 0.05 using a mixed effects model with temperature and dissolved oxygen as fixed factors The post-hoc Tukey results for dissolved oxygen are also presented Statistically significant P values are in bold directly measure or quantify bleaching, we did observe some paling in our hypoxic corals at the completion of these experiments. Massive corals are usually more tolerant to hypoxic conditions relative to branching coral species (Guzmán et al. 1990;Simpson et al. 1993;Andréfouët et al. 2001). Similarly, our results suggest that O. favelota is able to tolerate short-term exposure to hypoxia as we did not observe any mortality during our experiments and corals were still consuming oxygen albeit at a lower rate. The coral fragments used in this study were monitored for two months after the study concluded. None of the coral fragments died during that two month period suggesting that the response observed was indeed sub-lethal. The oxygen thresholds for exposure are unknown for most reef-building corals and only a few studies have reported the effects of both elevated temperature and hypoxia on coral physiology or survival (Hughes et al. 2020). This is likely because low oxygen events in the tropics are not as well categorized as hypoxic events documented in more temperate zones (Kealoha et al. 2020), even though tropical reef communities are particularly vulnerable to deoxygenation events (Hughes et al. 2020). Exposure to both hypoxia and elevated temperature simultaneously showed a negative response with an almost two times the reduction in oxygen demand in O. faveolota suggesting a trend toward a synergistic effect whereby increased temperature exacerbates the negative effect of hypoxia (post hoc result: P = 0.09, Table 2). Although some studies have described that O. faveolata appear highly resistant to ocean warming (Manzello et al. , 2018, exposure to hypoxic stress may accelerate bleaching and mortality. Temperatures are expected to increase five times faster in the twenty-first century compared with the 0.6°C warming rate documented for the twentieth century (Kerr et al. 2004) and some habitats (i.e., the Florida Keys) have already experienced a 0.8°C increase in sea surface temperature over the last century (Kuffner et al. 2015). Thermal stress, therefore, will undoubtedly decrease oxygen solubility in coastal habitats into the future, leading to more hypoxia, which may exacerbate some coral's ability to acclimate and survive additional increases in temperature. Future work should determine the long-term tolerance of O. faveolota to hypoxic stress and identify the coral's susceptibility to bleaching and disease when exposed to both stressors.
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