Pelagic deep-sea fauna observed on video transects in the southern Norwegian Sea

Observations of the diversity, distribution and abundance of pelagic fauna are absent for many ocean regions in the Atlantic, but baseline data are required to detect changes in communities as a result of climate change. Gelatinous fauna are increasingly recognized as vital players in oceanic food webs, but sampling these delicate organisms in nets is challenging. Underwater (in situ) observations have provided unprecedented insights into mesopelagic communities in particular for abundance and distribution of gelatinous fauna. In September 2018, we performed horizontal video transects (50–1200 m) using the pelagic in situ observation system during a research cruise in the southern Norwegian Sea. Annotation of the video recordings resulted in 12 abundant and 7 rare taxa. Chaetognaths, the trachymedusa Aglantha digitale and appendicularians were the three most abundant taxa. The high numbers of fishes and crustaceans in the upper 100 m was likely the result of vertical migration. Gelatinous zooplankton included ctenophores (lobate ctenophores, Beroe spp., Euplokamis sp., and an undescribed cydippid) as well as calycophoran and physonect siphonophores. We discuss the distributions of these fauna, some of which represent the first record for the Norwegian Sea.


Introduction
The pelagic ocean is the largest habitat on the planet and houses a high diversity, abundance and biomass of organisms. Up to 25% of the pelagic biomass can consist of gelatinous zooplankton (Robison 2004). Gelatinous organisms mainly consisting of the pelagic Cnidaria, Ctenophora and Tunicata have a variety of different body morphologies and feeding strategies, allowing them to occupy different niches in the oceanic foodweb (Haddock 2004). Pelagic tunicates (thaliaceans and appendicularians) are filter feeders (Holland 2016), while medusae, siphonophores and ctenophores are predators feeding on a wide spectrum of zooplankton including gelatinous organisms and micronekton (Pagès et al. 2006). Another prominent and widely distributed phylum in the oceanic water column are Chaetognatha, semi-gelatinous predators that can be among the numerically dominant plankton members of ocean communities (Bone et al. 1991).
Despite their low protein content, gelatinous zooplankton are common prey of many marine taxa including fishes, crustaceans, turtles and even some cephalopods (Heeger et al. 1992;Cardona et al. 2012;Hoving and Haddock 2017). The complex trophic interconnections of gelatinous zooplankton in the pelagic foodweb mark their central role within the pelagic ecosystem and their potential importance in the biological carbon pump (Alldredge 2004;Lebrato et al. 2012;Henschke et al. 2013;Sweetman and Chapman 2015). Gelatinous organisms have several traits that may give them an advantage in warmer, deoxygenated waters (Thuesen et al. 2005;Ekau et al. 2010). Although blooms of gelatinous organisms have been occurring for millennia, recent observations have drawn scientific and public interest to the question of whether or not the frequency of such blooming events is increasing, e.g. as a result of climate change (Lynam et al. 2011;Brotz et al. 2012;Condon et al. 2012Condon et al. , 2013. Confirming such potential increases requires a baseline of species diversity, distribution and abundance of gelatinous organisms. Fragile gelatinous zooplankton is often damaged or destroyed when sampled with nets, which has led to a systematic underestimation of its abundance and diversity in the ocean, in particular in the deep pelagic. The use of in situ observations (with optical sampling systems) has changed our perspective substantially (Haddock 2004;Robison 2004). Manned submersibles and remotely operated vehicles (ROVs) allow the observation of fragile gelatinous zooplankton in their natural habitat. Midwater ocean exploration with these vehicles has led to the discovery of new gelatinous species and records (e.g. Matsumoto and Robison 1992;Hoving et al. 2018), the documentation of their behaviour  and the provision of novel information on the trophic interactions in the deepsea 'jelly web' (Robison 2004;Choy et al. 2017;Hoving and Haddock 2017). Although submersibles and ROVs can provide detailed images and video material of large deep pelagic fauna and allow faunal collections (Robison 2004), they are also challenging and costly to operate. To allow cost-effective pelagic video transects comparable to ROV video transects, the pelagic in situ observation system (PELAGIOS) was developed (Hoving et al. 2019). The PELAGIOS is a camera platform that collects concomitant environmental data while towed horizontally at desired depths. The annotation of the high definition video results in diversity, distribution and abundance data of slow-swimming pelagic fauna larger than 1 cm. To establish a baseline of pelagic fauna distribution and abundance, and to identify potential important contributors (e.g. medusae, squids, larvaceans) to the biological carbon pump, we performed deep-sea pelagic video transects with PELAGIOS in the southern Norwegian Sea.

Material and methods
During a research cruise on R/V Heincke in September 2018 in the southern Norwegian Sea, we deployed the pelagic in situ observation system (PELAGIOS) four times to perform horizontal pelagic video transects ( Fig. 1; Table 1). The PELAGIOS is a battery powered towed camera platform with a high definition camera (type 1Cam Alpha, SubC Imaging), forward LED illumination, a CTD (SBE 19 Sea-CAT, Sea-Bird Scientific) and a telemetry (DST-6, Sea and Sun Technology; Linke et al. 2015) that transmits data and video preview via the CTD conducting cable to the deck unit (Hoving et al. 2019). The PELAGIOS was towed over the side of the ship on the CTD conducting cable at approximately 1 knot (0.51 m/s) speed over ground. The desired towing depth for the pelagic video transect was reached by paying out CTD wire while monitoring the depth from the CTD. The duration of one complete PELAGIOS deployment was between 3 h 11 min and 3 h 28 min, and at each depth we towed for approximately 6.5-23.3 min (Tables 1 and 2). Horizontal video transects were performed at specific depths between 50 and 1200 m (Table 2) and from 16:00 to 20:00 UTC (Table 1). We started with the deepest transect and ended shallow on Station_1. At all other stations we started shallow and ended with the deepest transect ( Table 2). Hydrography of the water column was reconstructed by performing a vertical cast with an onboard SBE CTD (Fig. 2). The HD video was annotated using the Video Annotation and Reference System (VARS) developed by the Monterey Bay Aquarium Research Institute (MBARI) (Schlining and Jacobsen Stout 2006). Recorded organisms larger than 1 cm in diameter were annotated and identified to the lowest possible taxonomic classification. Organisms observed while PELAGIOS was ascending or descending between transects were annotated but not considered in our data analysis. After the analysis of the video material the observation data were matched with the depth data and classified with the corresponding transects. This resulted in 15 abundant taxa (Table 3) and additional rare taxa, as well as groupings of unidentifiable organisms due to poor image conditions (Table 4).
To calculate abundances, we divided the absolute number of counts of each taxon per transect by the corresponding transect duration to obtain the relative abundance of taxa in individuals per time unit. To calculate the number of individuals per unit volume, we used faunal observations from simultaneous deployments of the PELAGIOS and a Underwater Vision Profiler (UVP5) together (Hoving et al. 2019). The UVP5 samples a fixed volume enabling a back calculation of the sampled volume of PELAGIOS during the same transect. This resulted in a conversion factor of 6. This factor is based on pelagic worms of the genus Poeobius (0.8-1.5 cm), which are poor swimmers and were observed clearly by both UVP5 and PELAGIOS (Christiansen et al. 2018;Hoving et al. 2019). The conversion factor may change when considering larger animals that can be seen from further away (Reisenbichler All graphs besides the map were produced in R version 3.6.1. The map was made with python and the packages matplotlib and cartopy. Differences between stations were analyzed by calculating the weighted mean depth for each station as well as an overall weighted mean for each of the 15 most abundant taxa (Latasa et al. 2017).

Hydrography
The hydrography was typical for the studied region (Blindheim and Rey 2004). The upper < 50 m were influenced by the Norwegian Coastal Current (NCC), with a salinity well below 34.9 and temperatures > 12 °C (Fig. 2). Below

Fauna
The video transects revealed 26 different taxa; fifteen abundant taxa (Table 3) and seven rare and seven unidentified taxa (Table 4). At all four sampling sites, the transects at 500, 600, 700 and 800 m depth had the highest animal abundances. Chaetognatha, which showed their highest abundances in these depths, dominated all transects with 68.8, 67.0, 63.0, and 64.6% of all annotations (Station_1 to Station_4, respectively) (Fig. 3). The small trachymedusa Aglantha digitale had its distribution peak between 500 and 800 m (Figs. 3 and 4) and accounted for 25.2, 22.4, 27.6 and 24.7% of all annotations (Station_1 to Station_4, respectively) and was the second most abundant taxon encountered in all transects. Appendicularians, crustaceans, ctenophores and siphonophores combined contributed about 10% of the observations between 500 and 800 m at Stations 2, 3 and 4, and less than 6% of the observations at Station_1. The 12 most abundant taxa differed in their vertical distribution patterns (Fig. 3). Non-gelatinous taxonomic groups included Actinopteri (ray-finned fishes), Decapoda and Mysida (jointly called shrimps, because distinction between the taxa was not possible on the videos), Euphausiacea (krill) and the semi-gelatinous Chaetognatha (arrow worms). Highest Actinopteri abundances were observed in the upper 100 m with a maximum of 267.6 ind 1000 m −3 at 75 m at Station_1, and abundances decreased with depth. Shrimps were observed at all stations and throughout the entire sampling depth range, but increased in numbers below 700 m, with a maximum of 76 ind 1000 m −3 at the single transect at 850 m depth at Station_4. A secondary abundance peak was observed at the deepest transect of 1200 m with 50.1 ind 1000 m −3 at Station_1. Euphausiacea were also observed at all stations and at every sampled depth, except at 1200 m. Their highest abundances were observed at 50 m depth at Station_1 with 5461.8 ind 1000 m −3 . Below 100 m, the abundance of Euphausiacea decreased by approximately one order of magnitude, and below 800 m depth by another order of magnitude.
Appendicularians were present throughout the sampled depth range with the exception of the 75 m transect at Station_1. Their numbers showed a continuous increase with depth, and peaked at 1000 m depth with 339.8 ind 1000 m −3 (mean, sd = 98.7). Chaetognaths were dominant between 200 and 1000 m. They were observed throughout the whole depth range (Fig. 3), and their vertical distribution pattern showed a normal distribution around an abundance peak at 700 m depth with 3350.5 ind 1000 m −3 (mean, sd = 1063.3). The highest numbers of pteropods of the genus Clione (157.6 ind 1000 m −3 ; mean, sd = 213.9) occurred at 50 m depth, but these mollusks were also present at 600 and 700 m. The trachymedusa Aglantha digitale was present between 50 and 1000 m depth but absent from the 75, 100 and 200 m transects (Figs. 3 and 4). Their highest abundance was observed at 800 m depth with 1507 ind 1000 m −3 (mean, sd = 963.5), and below 850 m depth the abundance decreased sharply. After chaetognaths, A. digitale was the second most abundant taxon. Small, unidentified calycophoran siphonophores were grouped together as 'other Calycophorae' and occurred between 300 and 1200 m depth. They were more abundant in the upper half of their distributional range and their peak abundance was 100.5 ind 1000 m −3 (mean, sd = 81.1) at 500 m depth. Calycophoran siphonophores of the family Diphyidae were restricted in their vertical distribution to the upper 200 m. They had their highest abundance at 100 m depth with 88.2 ind 1000 m −3 (mean, sd = 17.9). A group of physonect siphonophores with a similar morphology, but which could not be identified to species, were grouped as 'Physonect Deep-type' (Fig. 3). These siphonophores occurred from 400 to 1200 m depth, but the majority of specimens were observed at 1100 m depth with 107 ind 1000 m −3 at Station_1. Physonect siphonophores other than the 'Physonect Deep-type' were observed from 50 to 800 m as well as at 1000 m. Their abundance decreased with depth after a peak at 75 m (102.9 ind 1000 m −3 ) at Station_1. Beroe spp. ctenophores were observed in low numbers from 300 to 1200 m depth. The maximum abundance was 6.6 ind 1000 m −3 (mean, sd = 6.2) at 400 m depth. Some of the observed Beroe spp. could be clearly identified as B. abyssicola based on the conspicuous red coloration of the stomodaeum (Fig. 4). The habitus of the others points towards the unresolved species complex Beroe cf. cucumis (Bayha et al. 2004;Mills and Haddock 2007;Johansson et al. 2018), suggesting the presence of at least two species of Beroe in the study area. The lobate ctenophore Bolinopsis (Bolinopsis sp. or Bolinopsis infundibulum) was observed from 200 to 900 m depth. The maximum abundance of Bolinopsis was at 600 m with 10.1 ind 1000 m −3 (mean, sd = 8.3). The cydippid ctenophore Euplokamis sp. was observed 1 3  (Figs. 3 and 4). Their maximum abundance was 83.2 ind 1000 m −3 (mean, sd = 78.2) at 500 m depth. An undescribed white cydippid (Hosia and Båmstedt 2007) was observed from 600 to 800 m and from 1000 to1200 m depth (Figs. 3 and  4). Their highest abundance of 11.1 ind 1000 m −3 was observed during the deepest transect at 1200 m depth.
The major faunal components consisting of ray-finned fishes, euphausiids, shrimps, hydrozoans, ctenophores, chaetognaths and appendicularians, as well as the overall pelagic diversity and abundance, are in line with previous observations in the North Atlantic, where optical sampling was performed using ROV and UVP (Vinogradov 2005;Stemmann et al. 2008).
Our in situ video observations are generally limited to organisms that are > ~ 1 cm in size, and often do not show sufficient taxonomic characteristics required for identification to species level. Pelagic net surveys provide a higher taxonomic resolution for crustaceans and ray-finned fishes (Piatkowski et al. 1994), as well as for the hydrozoan gelatinous fauna (Hosia et al. , 2017, but catch efficiency may be low. On the other hand, optical sampling like performed in our study does reveal fauna poorly sampled by nets, ctenophores and appendicularians in particular, and provides unique data on the detailed vertical and horizontal distribution of fragile organisms (Hosia et al. 2017). The diversity of gelatinous fauna in the Norwegian Sea remains poorly studied, and more detailed faunistic studies would probably reveal new records of hydrozoans and ctenophores for Norwegian waters. In this study, significant numbers of the cydippid ctenophore Euplokamis sp. (n = 93) were documented for the first time in the southern Norwegian Sea. These ctenophores were readily identified to genus due to the characteristically coiled tentilla, resembling droplets along the tentacles (Fig. 2). There are few published records of Euplokamis sp. from Norwegian waters, but it is reported to occur in western Norwegian fjords (Granhag et al. 2012), and has been observed in the Norwegian /Icelandic Sea (Licandro et al. 2015), and near Svalbard (Majaneva and Majaneva 2013). First records of Euplokamis sp. in Swedish waters were reported for the Gullmar Fjord on the west coast (Granhag et al. 2012). Euplokamis may thus be present throughout the Norwegian shelf from the Skagerrak to Svalbard.
The other, yet undescribed, white cydippid ctenophore, similar to an undescribed horned cydippid with highly extensile tentacles (Hosia and Båmstedt 2007), was only observed below 600 m depth, with highest densities at the 1200 m transect at Station_1. This suggests that the vertical distribution was not completely sampled, and that the undescribed cydippid is a lower meso-and bathypelagic species with a distribution extending below our sampling range.
Several taxa appeared to be associated with specific water masses. Shrimps were primarily found in NSDW below 600 m. The undescribed white cydippid and the siphonophore that we referred to as the 'Physonect Deep-type' also appeared mostly restricted to these deeper waters. Of the numerically dominant groups, both chaetognaths and Aglantha digitale had their peak abundances below 600 m depth, in the NSDW. A. digitale was virtually absent from AW, while chaetognaths also extended their distribution to the upper 400 m, perhaps due to this taxon including several species of varying affinity to the different water masses. Euphausiacea, Clione and diphyid siphonophores were primarily observed in the upper AW. Other calycophorans and Euplokamis sp. were particularly abundant in the intermediate waters, but also occurred, to a lesser extent, in the water masses above and below.
Appendicularians were a common group, increasing in abundance with depth throughout the mesopelagic zone. Abundant appendicularians in mesopelagic waters are also reported from other oceanic regions, but are relatively poorly studied . The role of appendicularians in mesopelagic food webs and in vertical flux is nevertheless of high interest. Not only are appendicularians able to feed on particles down to bacterial size range, thus short circuiting the "normal" pelagic food chain (Robinson et al. 2010), but their discarded houses are a major contributor of marine snow particles and vertical flux of carbon (Robison et al. 2005;De La Rocha and Passow 2007).
For some of the observed taxa, potential diel vertical migration (DVM) must also be considered. While relatively detailed, our vertical distribution data only provide a temporal snapshot. Ray-finned fishes, krill, shrimps and physonect siphonophores are known daily vertical migrators (Barham 1966;Piatkowski et al. 1994;Frank and Widder 2002), and their observed distributions in the upper layers may have been indicative of these taxa migrating towards the surface as sunset was approaching. While our data do not provide information on diel changes in the vertical distribution of the observed taxa, deployments were more or less consistent in their timing with respect to the sunset and, thus, the expected stage of DVM. As DVM of many taxa is likely controlled by the ambient light environment (e.g. Norheim et al. 2016), between-day variation may nevertheless have be caused by differences in cloud cover. However, the significant differences found in the distribution of ray-finned fishes were likely caused by the fact that we started the transects in deep waters during the first station, while we started the later stations in shallow waters.
When considering the observed depth distributions and extrapolated abundances, the total number of observations should be taken into account. These data are much more uncertain for the less frequent taxa. Also, the deepest layers at 1000 m or below only had 1-2 transects per depth, preventing comparisons between sites and general conclusions. Due to the relatively coarse taxonomic resolution of the data, many of the taxa are also likely to contain several species, with potentially different environmental preferences, ecological niches and behaviour. For example, three species of krill are common and abundant in the Norwegian Sea: Meganyctiphanes norvegica, Thysanoessa inermis and Thysanoessa longicaudata (Melle et al. 2004). In our data, these and other similar organisms were lumped. Comparing abundance estimates obtained by different studies is challenging, as they may be affected by a variety of factors including e.g. the sampling methods, interannual and geographical variation, as well as diurnal and/or seasonal changes in abundance and distribution of the target taxa. With these caveats in mind, we can make some comparisons to published data for our most abundant groups. Vertical distributions of chaetognaths in the Norwegian and Greenland Seas have been studied in November 1995 using a Multinet, a multiple plankton net (Dale et al. 1999). The results are surprisingly similar to ours, with maximum densities of 3-4 chaetognaths m −3 observed between 400 and 800 m depth. For Aglantha digitale, ROV vertical profilesa method comparable to ours in that it is limited to observing organisms larger than 1 cm in size-from Norwegian fjords in October andMay 2004-2005 show peaks of up to ~ 3 adult individuals m −3 at around 300 m depth (Hosia and Båmstedt 2007). These results are also of the same order of magnitude as ours, although the exact depth of the peak abundance differs, perhaps due to local hydrography. The abundance estimates of chaetognaths and A. digitale may be realistic because the size of these organisms is similar to the Poeobius polychaete that was used for estimating sampling volume of PELAGIOS (Hoving et al. 2019). Estimating abundance of larger fauna requires further calibration efforts of PELAGIOS as larger fauna may be detected when they are further away from the camera compared to smaller organisms (Reisenbichler et al. 2016).
Our results may to a degree be influenced by attraction or avoidance responses elicited by the PELAGIOS. Such responses could result from the hydrodynamic disturbance caused by the gear, as well as the light. It is known that certain taxa such as ray-finned fishes, krill and decapods, respond to underwater instruments (Stoner et al. 2008). Underestimation of observations may influence our estimates of myctophid and other mesopelagic fishes as they are known to avoid lights and trawls (Kaartvedt et al. 2012). On the other hand, overestimation of abundance may be caused by organisms being attracted towards lights or the instrument itself, as has been reported for other organisms including krill and some ray-finned fishes (e.g. Wiebe et al. 2004;Raymond and Widder 2007;Utne-Palm et al. 2018). However, we did not observe highly reactive responses to the lights.
We have noticed the absence of larger nekton. This particularly is relevant to the oegopsid squid Gonatus fabricii, which is widely distributed in the North Atlantic and adjacent Arctic seas and is the dominant cephalopod in terms of biomass, and a pivotal component in the food web (Kristensen 1984;Wiborg et al. 1984). Gonatus fabricii is consumed by a variety of apex predators such as various cetaceans and deep-sea fishes (Lick and Piatkowski 1998;Santos et al. 1999;Bergstad et al. 2010). It is estimated that sperm whale populations in the north east Atlantic alone consume as much as 1.5 million tons of G. fabricii each year (Bjørke 2001). Our sampling site was chosen because previous studies reported mature and spent females of G. fabricii in the area (Arkhipkin and Bjørke 1999;Bjørke 2001). The absence of G. fabricii could be caused by an avoidance of PELAGIOS resulting in an escape response before specimens are within the view-field of the camera. It is also possible that the sampling volume of the PELAGIOS system and the transects were not sufficient to document the species. However, it should be noted that Gonatus steenstrupi was recorded by ROVs during dives at the northern mid-Atlantic ridge (Vecchione et al. 2010). We furthermore did not observe the coronate medusa Periphylla periphylla, which occurs at high densities in several Norwegian fjord systems (e.g. Båmstedt et al. 2003), and which is also common in the Norwegian Sea in depths below 200 m (Dalpadado et al. 1998). The absence of P. periphylla could be the result of patchy distribution or by avoidance behaviour.
Our results provide a first overview of the vertical composition, distribution and abundance of macroplankton in the Norwegian Sea. We show that predatory and, to a lesser degree, filter feeding non-crustacean zooplankton dominate the mesopelagic macroplankton community of the study area. The resultant vertical abundance distributions may be useful for modelling trophic pathways and vertical flux in the mesopelagic. More detailed, net-or ROV-based studies are necessary in order to describe the currently poorly known species level diversity of gelatinous zooplankton in this region. Additionally, the absence of observations of the abundant squid G. fabricii asks for a more intense sampling program to better understand the biology of this important species.

Conflict of interest
The authors declare that they have no conflict of interest to disclose.
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