Abstract
Key Message
This study identified 16 pyridoxal phosphate-dependent decarboxylases in olive at the whole-genome level, conducted analyses on their physicochemical properties, evolutionary relationships and characterized their activity.
Abstract
Group II pyridoxal phosphate-dependent decarboxylases (PLP_deC II) mediate the biosynthesis of characteristic olive metabolites, such as oleuropein and hydroxytyrosol. However, there have been no report on the functional differentiation of this gene family at the whole-genome level. This study conducted an exploration of the family members of PLP_deC II at the whole-genome level, identified 16 PLP_deC II genes, and analyzed their gene structure, physicochemical properties, cis-acting elements, phylogenetic evolution, and gene expression patterns. Prokaryotic expression and enzyme activity assays revealed that OeAAD2 and OeAAD4 could catalyze the decarboxylation reaction of tyrosine and dopa, resulting in the formation of their respective amine compounds, but it did not catalyze phenylalanine and tryptophan. Which is an important step in the synthetic pathway of hydroxytyrosol and oleuropein. This finding established the foundational data at the molecular level for studying the functional aspects of the olive PLP_deC II gene family and provided essential gene information for genetic improvement of olive.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Funding
This work was supported by Fundamental Research Funds for the Central Nonprofit Research Institution of the Chinese Academy of Forestry (CAFYBB2023PA005-2); National Natural Science Foundation of China (32371837) and Fundamental Research Funds for the Central Nonprofit Research Institution of the Chinese Academy of Forestry (CAFYBB2021QC001).
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Guodong Rao planned and designed the research; Qizhen Cui, Qingqing Liu, Yutong Fan, Chenhe Wang, Yufei Li and Shuyuan Li designed and performed the experiments; Qizhen Cui analyzed the data and wrote the original manuscript; Guodong Rao and Jianguo Zhang oversaw and managed the research activity and reviewed the manuscript. All authors have read and agreed to the published version of the manuscript.
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Cui, Q., Liu, Q., Fan, Y. et al. Functional differentiation of olive PLP_deC genes: insights into metabolite biosynthesis and genetic improvement at the whole-genome level. Plant Cell Rep 43, 127 (2024). https://doi.org/10.1007/s00299-024-03212-z
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DOI: https://doi.org/10.1007/s00299-024-03212-z