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Comparative study of catalase immobilization via adsorption on P(MMA-co-PEG500MA) structures as an effective polymer support

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Abstract

In this study, poly[methyl methacrylate-co-poly(ethylene glycol) methacrylate] (P(MMA-co-PEG500MA)) copolymers were used for catalase (CAT) immobilization. Firstly, P(MMA-co-PEG500MA) copolymers were synthesized by using different amount of methyl methacrylate (MMA) and poly(ethylene glycol) methacrylate (PEG500MA) monomers. The synthesized copolymers were characterized by different analysis techniques. Afterward, CAT enzyme was immobilized via physical adsorption method onto the P(MMA-co-PEG500MA) copolymers. P3 sample containing 1:1 (PEG500MA:MMA) monomer molar ratio was selected as model support because of exhibiting optimum surface porosity and thermal stability. A high immobilization yield (76%) was achieved under optimized conditions. The immobilized enzyme displayed improved tolerance towards pH and temperature changes. After immobilization, the optimum pH shifted from 7.5 to 7.0, whereas the optimum temperature remained unchanged at 35 °C. Immobilized enzyme showed good reuse potential and excellent storage stability. After 10 consecutive uses, immobilized enzyme maintained about 51.0% of its initial activity. Furthermore, free enzyme completely lost its initial activity after 4 weeks, while immobilized enzyme maintained approximately 65% of the initial activity at 25 °C. Approximately twofold decrease in Km was obtained which means that the affinity of enzyme to the substrate improved after immobilization. Finally, it can be concluded that the prepared P(MMA-co-PEG500MA) copolymer structure can be an ideal matrix for CAT immobilization.

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References

  1. Koushal V, Sharma R, Sharma M, Sharma R, Sharma V (2014) Plastics: issues challenges and remediation. Int J Waste Resour 4(1):134

    Google Scholar 

  2. Raheem D (2012) Application of plastics and paper as food packaging materials—an overview. Emir J Food Agric 25(3):177–188

    Google Scholar 

  3. Mülhaupt R (2013) Green polymer chemistry and bio-based plastics: dreams and reality. Macromol Chem Phys 214:159–174

    Google Scholar 

  4. Ignatyev IA, Thielemans W, Vander Beke B (2014) Recycling of polymers: a review. ChemSusChem 7:1579–1593

    CAS  PubMed  Google Scholar 

  5. Luzi F, Torre L, Kenny JM, Puglia D (2019) Bio- and fossil-based polymeric blends and nanocomposites for packaging: structure–property relationship. Materials (Basel) 12(3):471

    CAS  Google Scholar 

  6. Othman SH (2014) Bio-nanocomposite materials for food packaging applications: types of biopolymer and nano-sized filler. Agric Agric Sci Procedia 2:296–303

    Google Scholar 

  7. Fan B, Zhou M, Zhang C, He D, Bai J (2019) Polymer-based materials for achieving high energy density film capacitors. Prog Polym Sci 97:101143

    CAS  Google Scholar 

  8. Balakrishnan P, Geethamma VG, Sreekala MS, Thomas S (2018) Polymeric biomaterials: state-of-the-art and new challenges. In: Thomas S, Balakrishnan P, Sreekala MS (eds) Woodhead publishing series in biomaterials, fundamental biomaterials: polymers. Woodhead Publishing, Sawston, pp 1–20

    Google Scholar 

  9. Shah SA, Sohail M, Khan S, Minhas MU, de Matas M, Sikstone V, Hussain Z, Abbasi M, Kousar M (2019) Biopolymer-based biomaterials for accelerated diabetic wound healing: a critical review. Int J Biol Macromol 139:975–993

    CAS  PubMed  Google Scholar 

  10. Abbasian M, Massoumi B, Mohammad-Rezaei R, Samadian H, Jaymand M (2019) Scaffolding polymeric biomaterials: are naturally occurring biological macromolecules more appropriate for tissue engineering? Int J Biol Macromol 134:673–694

    CAS  PubMed  Google Scholar 

  11. Feng C, Huang X (2018) Polymer brushes: efficient synthesis and applications. Acc Chem Res 51(9):2314–2323

    CAS  PubMed  Google Scholar 

  12. Advincula RC, Brittain WJ, Caster KC, Ruehe J (2004) Polymer brushes. Wiley-VCH, Weinheim

    Google Scholar 

  13. Milner ST (1991) Polymer brushes. Science 251:905–914

    CAS  PubMed  Google Scholar 

  14. Zhao B, Brittain WJ (2000) Polymer brushes: surface-immobilized macromolecules. Prog Polym Sci 25:677–710

    CAS  Google Scholar 

  15. Bumbu GG, Kircher G, Wolkenhauer M, Berger R, Gutmann JS (2004) Synthesis and characterization of polymer brushes on micromechanical cantilevers. Macromol Chem Phys 205:1713–1720

    CAS  Google Scholar 

  16. Krishnamoorthy M, Hakobyan S, Ramstedt M, Gautrot JE (2014) Surface-initiated polymer brushes in the biomedical field: applications in membrane science, biosensing, cell culture, regenerative medicine and antibacterial coatings. Chem Rev 114:10976–11026

    CAS  PubMed  Google Scholar 

  17. Koenig M, König U, Eichhorn K-J, Müller M, Stamm M, Uhlmann P (2019) In-situ-investigation of enzyme immobilization on polymer brushes. Front Chem 7(101):1–8

    Google Scholar 

  18. Koenig M, Bittrich E, König U, Rajeev BL, Müller M, Eichhorn K-J, Thomas S, Stamm M, Uhlmann P (2016) Adsorption of enzymes to stimuli-responsive polymer brushes: influence of brush conformation on adsorbed amount and biocatalytic activity. Colloids Surf B Biointerfaces 146:737–745

    CAS  PubMed  Google Scholar 

  19. del Castillo GF-D, Koenig M, Müller M, Eichhorn K-J, Stamm M, Uhlmann P, Dahlin A (2019) Enzyme immobilization in polyelectrolyte brushes: high loading and enhanced activity compared to monolayers. Langmuir 35:3479–3489

    Google Scholar 

  20. Xu FJ, Cai QJ, Li YL, Kang ET, Neoh KG (2005) Covalent immobilization of glucose oxidase on well-defined poly(glycidyl methacrylate)−Si(111) hybrids from surface-initiated atom-transfer radical polymerization. Biomacromolecules 6(2):1012–1020

    CAS  PubMed  Google Scholar 

  21. Qin W, Song Z, Fan C, Zhang W, Cai Y, Zhang Y, Qian X (2012) Trypsin immobilization on hairy polymer chains hybrid magnetic nanoparticles for ultra fast, highly efficient proteome digestion, facile 18O labeling and absolute protein quantification. Anal Chem 847:3138–3144

    Google Scholar 

  22. Rafiee-Pour HA, Nejadhosseinian M, Firouzi M, Masoum S (2019) Catalase immobilization onto magnetic multi-walled carbon nanotubes: optimization of crucial parameters using response surface methodology. New J Chem 43:593–600

    CAS  Google Scholar 

  23. Arabaci G, Usluoglu A (2013) Catalytic properties and immobilization studies of catalase from Malva sylvestris L. J Chem 686185:1–6

    Google Scholar 

  24. Czechowska E, Ranoszek-Soliwoda K, Tomaszewska E, Pudlarz A, Celichowski G, Gralak-Zwolenik D, Szemraj J, Grobelny J (2018) Comparison of the antioxidant activity of catalase immobilized on gold nanoparticles via specific and non-specific adsorption. Colloids Surf B Biointerfaces 171:707–714

    CAS  PubMed  Google Scholar 

  25. Zhang L, Sun Y (2018) Poly(carboxybetaine methacrylate)-grafted silica nanoparticle: a novel carrier for enzyme immobilization. Biochem Eng J 132:122–129

    CAS  Google Scholar 

  26. Costa SA, Reis RL (2004) Immobilisation of catalase on the surface of biodegradable starch-based polymers as a way to change its surface characteristics. J Mater Sci Mater Med 15(4):335–342

    CAS  PubMed  Google Scholar 

  27. Kaushal J, Seema SG, Arya SK (2018) Immobilization of catalase onto chitosan and chitosan–bentonite complex: a comparative study. Biotechnol Rep 18(e00258):1–6

    Google Scholar 

  28. Inanan T (2019) Chitosan co-polymeric nanostructures for catalase immobilization. React Funct Polym 135:94–102

    CAS  Google Scholar 

  29. Feng Y, Zhong L, Bilal M, Tan Z, Hou Y, Jia S, Cui J (2018) Enzymes@ZIF-8 nanocomposites with protection nanocoating: stability and acid-resistant evaluation. Polymers (Basel) 11:1–14

    Google Scholar 

  30. Feng Q, Zhao Y, Wei A, Li C, Wei Q, Fong H (2014) Immobilization of catalase on electrospun PVA/PA6–Cu(II) nanofibrous membrane for the development of efficient and reusable enzyme membrane reactor. Environ Sci Technol 48(17):10390–10397

    CAS  PubMed  Google Scholar 

  31. Bayramoglu G, Karagoz B, Yilmaz M, Bicak N, Arica MY (2011) Immobilization of catalase via adsorption on poly(styrene-d-glycidylmethacrylate) grafted and tetraethyldiethylenetriamine ligand attached microbeads. Bioresour Technol 102(4):3653–3661

    CAS  PubMed  Google Scholar 

  32. Murtinho D, Lagoa AR, Garcia FAP, Gil MH (1998) Cellulose derivatives membranes as supports for immobilisation of enzymes. Cellulose 5:299–308

    CAS  Google Scholar 

  33. Airoldi C, Monteiro Junior O (2003) Copper adsorption and enzyme immobilization on organosilane-glutaraldehyde hybrids as support. Polym Bull 50:61–68

    CAS  Google Scholar 

  34. Ingenbosch KN, Rousek A, Wunschik DS, Hoffmann-Jacobsen K (2019) A fluorescence-based activity assay for immobilized lipases in non-native media. Anal Biochem 569:22–27

    CAS  PubMed  Google Scholar 

  35. Cerqueira MRF, Santos MSF, Matos RC, Gutz IGR, Angnes L (2015) Use of poly(methyl methacrylate)/polyethyleneimine flow microreactors for enzyme immobilization. Microchem J 118:231–237

    CAS  Google Scholar 

  36. Li S, Hu J, Liu B (2004) Use of chemically modified PMMA microspheres for enzyme immobilization. Biosystems 77(1–3):25–32

    CAS  PubMed  Google Scholar 

  37. Bulmuş V, Ayhan H, Pişkin E (1997) Modified PMMA monosize microbeads for glucose oxidase immobilization. Chem Eng J Biochem Eng J 65(1):71–76

    Google Scholar 

  38. Lai Y, Wang F, Zhang Y, Ou P, Wu P, Fang Q, Li S, Chen Z (2019) Effective removal of methylene blue and orange II by subsequent immobilized laccase decolorization on crosslinked polymethacrylate/carbon nanotubes. Mater Res Express 6(085541):1–11

    Google Scholar 

  39. Pugazhenthi G, Kumar A (2004) Enzyme membrane reactor for hydrolysis of olive oil using lipase immobilized on modified PMMA composite membrane. J Membr Sci 228:187–197

    CAS  Google Scholar 

  40. Ulu A, Koytepe S, Ates B (2016) Synthesis and characterization of PMMA composites activated with starch for immobilization of L-asparaginase. J Appl Polym Sci 43421:1–11

    Google Scholar 

  41. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    CAS  PubMed  Google Scholar 

  42. Patil PD, Yadav GD (2018) Rapid in situ encapsulation of laccase into metal-organic framework support (ZIF-8) under biocompatible conditions. ChemistrySelect 3:4669–4675

    CAS  Google Scholar 

  43. Kanimozhi G, Vinoth S, Harish K, Srinadhu E, Satyanarayana N (2019) Conductivity and dielectric permittivity studies of KI-based nanocomposite (PEO/PMMA/KI/I2/ZnO nanorods) polymer solid electrolytes. Polym Compos 40:2919–2928

    CAS  Google Scholar 

  44. Abdelrazek E, Elashmawi I, Soliman M, Aly A (2009) Physical properties of MnCl2 fillers incorporated into a PVDF/PVC blend and their complexes. J Vinyl Addit Technol 15:171–177

    CAS  Google Scholar 

  45. Poomalai P, Varghese TO, Siddaramaiah (2011) Thermomechanical behaviour of poly(methylmethacrylate)/copoly(ether-ester) blends. Int Sch Res Netw ISRN Mater Sci 2011:1–5

    Google Scholar 

  46. Ramírez-Jiménez A, Montoya-Villegas KA, Licea-Claverie A, Gónzalez-Ayón MA (2019) Tunable thermo-responsive copolymers from DEGMA and OEGMA synthesized by RAFT polymerization and the effect of the concentration and saline phosphate buffer on its phase transition. Polymers 11:1657

    PubMed Central  Google Scholar 

  47. Tsai B, Garcia-Valdez O, Champagne P, Cunningham MF (2017) Poly(poly(ethylene glycol) methyl ether methacrylate) grafted chitosan for dye removal from water. Processes 5(12):1–13

    Google Scholar 

  48. Asmat S, Husain Q, Azam A (2017) Lipase immobilization on facile synthesized polyaniline-coated silver-functionalized graphene oxide nanocomposites as novel biocatalysts: stability and activity insights. RSC Adv 7:5019–5029

    CAS  Google Scholar 

  49. Saleem M, Rafiq M, Seo SY, Lee KH (2016) Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart. Biosci Rep 36(art:00311):1–11

    Google Scholar 

  50. Patel SKS, Otari SV, Chan Kang Y, Lee JK (2017) Protein-inorganic hybrid system for efficient his-tagged enzymes immobilization and its application in l-xylulose production. RSC Adv 7:3488–3494

    CAS  Google Scholar 

  51. Samui A, Sahu SK (2018) One-pot synthesis of microporous nanoscale metal organic frameworks conjugated with laccase as a promising biocatalyst. New J Chem 42:4192–4200

    CAS  Google Scholar 

  52. Çetinus ŞA, Öztop HN, Saraydin D (2007) Immobilization of catalase onto chitosan and cibacron blue F3GA attached chitosan beads. Enzyme Microb Technol 41(4):447–454

    Google Scholar 

  53. Erol K, Cebeci BK, Köse K, Köse DA (2019) Effect of immobilization on the activity of catalase carried by poly(HEMA-GMA) cryogels. Int J Biol Macromol 123:738–743

    CAS  PubMed  Google Scholar 

  54. Kumar A, Patel SKS, Mardan B, Pagolu R, Lestari R, Jeong SH, Kim T, Haw JR, Kim SY, Kim IW, Lee JK (2018) Immobilization of xylanase using a protein-inorganic hybrid system. J Microbiol Biotechnol 28(4):638–644

    CAS  PubMed  Google Scholar 

  55. Sharma N (2013) Kinetic study of free and immobilized protease from Aspergillus sp. IOSR J Pharm Biol Sci 7(2):86–96

    Google Scholar 

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  1. Department of Chemistry, Faculty of Science and Literature, Inonu University, 44280, Malatya, Turkey

    Evren Sel, Ahmet Ulu, Burhan Ateş & Süleyman Köytepe

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  1. Evren Sel
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Sel, E., Ulu, A., Ateş, B. et al. Comparative study of catalase immobilization via adsorption on P(MMA-co-PEG500MA) structures as an effective polymer support. Polym. Bull. 78, 2663–2684 (2021). https://doi.org/10.1007/s00289-020-03233-0

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