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Description and Genomic Characteristics of Diaphorobacter limosus sp. nov., Isolated from a Sewage-Treatment Plant

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Abstract

A Gram-stain-negative, rod-shaped, non-motile, catalase-positive, denitrifying bacterium, designated strain Y-1T, was isolated from an aeration tank of a sewage treatment plant in China and characterized using polyphasic taxonomic approaches. Strain Y-1T could grow at 10–37 °C (optimum 25 °C), at pH 5.0–10.0 (optimum 7.0) and in the presence of 0–3.0% (w/v) NaCl (optimum 0.5%). The phylogenetic tree based on the 16S rRNA gene sequences revealed that strain Y-1T was a member of genus Diaphorobacter, and showed the highest sequence similarities with Diaphorobacter oryzae RF3T (97.50%), Diaphorobacter nitroreducens NA10BT (97.38%) and Diaphorobacter aerolatus 8604S-37T (96.56%). In terms of carbon source utilization and enzyme activities, strain Y-1T was significantly different from its similar strains. The major respiratory quinone was Q-8, and the main polar lipid was phosphatidylethanolamine. Comparative genomic analysis of strain Y-1T and other Diaphorobacter species was conducted to explore the mechanisms underlying the differences among these strains. Strain Y-1T encoded 3957 genes, consisting of 3813 protein-coding genes and 144 RNA coding genes, and encoded 652 enzymes with 31 unique enzymes compared with other related species. The DNA G + C content was 69.95 mol%. Strain Y-1T exhibited 41.71% DNA–DNA relatedness and 95% ANIb with the most related type strains.

On the basis of the evidence presented from polyphasic analysis, strain Y-1T was suggested as a novel species within the genus Diaphorobacter, for which the name Diaphorobacter limosus sp. nov. is proposed, with the type strain Y-1T (= KCTC 92852T = CCTCC AB 2023032T).

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Funding

This study was supported by the Key Realm Research and Development Program of Guangdong Province (2023B0202020001, 2020B0202080001), National Natural Science Foundation of China (42307006, 42177208), Natural Science Foundation of Guangdong Province, China (2022A1515011929), the Guangdong Laboratory for Lingnan Modern Agriculture Project (NT2021010, Heyuan Branch DT20220002), Science and Technology Project of Heyuan (Heke 2021001), Guangdong Provincial Science and Technology Plan Project (2021B1212040008), and Guangdong Special Support Program (2021TQ060163).

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Author notes

  1. Kaiqing Yang and Jialin Tan have contribute equally to this work.

Authors and Affiliations

  1. Heyuan Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Agricultural and Rural Pollution Abatement and Environmental Safety, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, China

    Kaiqing Yang, Jialin Tan, Zhuobiao Ni, Qingqi Lin, Zhepu Ruan & Rongliang Qiu

  2. School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou, 510006, China

    Gengbo Jiang, Yuanqing Chao & Rongliang Qiu

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  1. Kaiqing Yang
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  2. Jialin Tan
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Contributions

KY wrote the manuscript. JT isolated the strain Y-1T. KY, JT, and GJ performed the experiments. ZN, QL, QC, ZR, and RQ revised the manuscript. ZR and RQ supervised the study. All authors read and approved the final manuscript.

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Correspondence to Zhepu Ruan or Rongliang Qiu.

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Yang, K., Tan, J., Jiang, G. et al. Description and Genomic Characteristics of Diaphorobacter limosus sp. nov., Isolated from a Sewage-Treatment Plant. Curr Microbiol 81, 155 (2024). https://doi.org/10.1007/s00284-024-03659-z

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