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Hymenobacter guriensis sp. nov., and Hymenobacter duratus sp. nov., Radiation-Resistant Species Isolated from Soil in South Korea

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Abstract

Two novel Gram-stain-negative, non-motile, aerobic, rod-shaped, circular, convex, red-colored and UV-tolerant strains BT594T and BT646T were isolated from soil collected in Guri city (37° 36′ 0″ N, 127° 9′ 0″ E) and Gwangju city (37° 22′ 0″ N, 127° 17′ 0″ E), respectively, South Korea. 16S rDNA sequence analysis indicated that strains BT594T and BT646T belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia, phylum Bacteroidetes, kingdom Bacteria). The 16S rDNA gene sequence similarity between the two strains BT594T and BT646T was 96.2%. The strain BT594T was closely related to Hymenobacter psychrotolerans Tibet-IIU11T (97.0% 16S rDNA gene similarity) and Hymenobacter tibetensis XTM003T (96.3%). The strain BT646T was closely related to Hymenobacter psychrotolerans Tibet-IIU11T (98.6%), Hymenobacter kanuolensis T-3 T (96.8%) and Hymenobacter perfusus LMG 26000 T (96.7%). The two strains were found to have the same quinone system, with MK-7 as the major respiratory quinone. The major polar lipids of strains BT594T and BT646T were phosphatidylethanolamine (PE) and aminophospholipids (APL). The major cellular fatty acids of strain BT594T were anteiso-C15:0 (17.9%), iso-C15:0 (16.1%) and summed feature 3 (C16:1 ω6c / C16:1 ω7c) (10.0%). The major cellular fatty acids of strain BT646T were summed feature 3 (C16:1 ω6c / C16:1 ω7c) (18.3%), C16:0 (17.2%) and summed feature 4 (iso-C17:1 I / anteiso-C17:1 B) (14.5%). Based on the polyphasic analysis, strains BT594T and BT646T can be suggested as two novel bacterial species within the genus Hymenobacter and the proposed names are Hymenobacter guriensis and Hymenobacter duratus, respectively. The type strain of Hymenobacter guriensis is BT594T (= KCTC 21863 T = NBRC 114853 T) and the type strain of Hymenobacter duratus is BT646T (= KCTC 21915 T = NBRC 114854 T).

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Acknowledgements

This work was supported by a research grant from Seoul Women’s University (2021) and by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002108). This research was supported by the MIST (Ministry of Science, ICT), Korea, under the National Program for Excellence in SW), supervised by the IITP (Institute of Information & communications Technology Planing & Evaluation) in 2021 (2016-0-00022). We are grateful to Dr. Aharon Oren (The Hebrew University of Jerusalem, Israel) for helping with the etymology.

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  1. Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 139-774, South Korea

    Tuvshinzaya Damdintogtokh & Myung Kyum Kim

  2. National Institute of Biological Resources, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, South Korea

    In-Tae Cha

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  1. Tuvshinzaya Damdintogtokh
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Correspondence to In-Tae Cha or Myung Kyum Kim.

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Damdintogtokh, T., Cha, IT. & Kim, M.K. Hymenobacter guriensis sp. nov., and Hymenobacter duratus sp. nov., Radiation-Resistant Species Isolated from Soil in South Korea. Curr Microbiol 78, 3334–3341 (2021). https://doi.org/10.1007/s00284-021-02517-6

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