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Advances in hematopoietic stem cells ex vivo expansion associated with bone marrow niche

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Abstract

Hematopoietic stem cells (HSCs) are an ideal source for the treatment of many hematological diseases and malignancies, as well as diseases of other systems, because of their two important features, self-renewal and multipotential differentiation, which have the ability to rebuild the blood system and immune system of the body. However, so far, the insufficient number of available HSCs, whether from bone marrow (BM), mobilized peripheral blood or umbilical cord blood, is still the main restricting factor for the clinical application. Therefore, strategies to expand HSCs numbers and maintain HSCs functions through ex vivo culture are urgently required. In this review, we outline the basic biology characteristics of HSCs, and focus on the regulatory factors in BM niche affecting the functions of HSCs. Then, we introduce several representative strategies used for HSCs from these three sources ex vivo expansion associated with BM niche. These findings have deepened our understanding of the mechanisms by which HSCs balance self-renewal and differentiation and provided a theoretical basis for the efficient clinical HSCs expansion.

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Notes

  1. FP6: an artificially generated IL-6/sIL-6R fusion protein.

  2. SRCs: SCID (severe combined immunodeficient) repopulating cells.

  3. FBS: fetal bovine serum.

  4. HPCs: hematopoietic progenitor cells.

  5. LTR-HSCs: long-term repopulating hematopoietic stem cells.

  6. TNCs: total nucleated cells.

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Funding

This study was supported by the Central Guidance for Local Science and Technology Development Projects in Shanxi Province (No. YDZJSX2021B009), the Key Project of Science and Technology of Shanxi Province (No. 2021XM07), and the Fund Program for the Scientific Activities of Selected Returned Overseas Professionals in Shanxi Province (No. 20210029).

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Authors and Affiliations

  1. Institute of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China

    Ju Deng, Yanhong Tan, Zhifang Xu & Hongwei Wang

  2. The Key Laboratory of Molecular Diagnosis and Treatment of Hematological Disease of Shanxi Province, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China

    Ju Deng, Yanhong Tan, Zhifang Xu & Hongwei Wang

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  1. Ju Deng
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  2. Yanhong Tan
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  4. Hongwei Wang
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All authors contributed to the study conception and design. The first draft of the manuscript was written by J.D. and Y.T. Z.X. and H.W. edited the manuscript. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Hongwei Wang.

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Deng, J., Tan, Y., Xu, Z. et al. Advances in hematopoietic stem cells ex vivo expansion associated with bone marrow niche. Ann Hematol (2024). https://doi.org/10.1007/s00277-024-05773-1

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