Rare gene variants in a patient with azathioprine-induced lethal myelosuppression

Dear Editor, A 46-year-old Chinese male patient was diagnosed with autoimmune hepatitis—primary biliary cholangitis overlap syndrome at the first admission on Apr 22nd 2016. He was treated with ursodeoxycholic acid, followed by prednisolone and azathioprine (Fig. 1a). He was admitted again with fever and sore throat on Sept 15th, showing visible throat swelling. Notably, a sharp drop of blood cells was observed, reaching the diagnostic criteria of grade 4 myelosuppression (Fig. 1a), with 0.18 × 10/L for neutrophilic granulocytes. Azathioprine and prednisolone were stopped immediately, while human granulocyte colony-stimulating factor and recombinant human thrombopoietin were prescribed to increase blood cells. However, the patient still suffered a life-threatening progressive decrease of blood cells. The lowest level of neutrophilic granulocytes, reticulocytes, and white blood cells reached 0.01 × 10/L, 0.0040 × 10/L, 0.25 × 10/L, respectively, on Sept 23rd, while the hemoglobin, platelets, and red blood cells dropped to the lowest level at 54 g/L, 22*10/L, and 1.66*10/L, respectively, on Oct 1st (Fig. 1a). Moreover, diffuse rash and severe alopecia were observed. Serious infections (Pseudomonas aeruginosa infection) also occurred during hospitalization. The blood counts recovered far more slowly than expected and it returned to normal until 25 days (Oct 10th) after therapy (Fig. 1a). We attempted to genotype the SNPs of NUDT15 (rs116855232) and TPMT (rs1142345) of this patient and found that this patient has NUDT15 TPMT wt /risk genotype (Fig. 1b). The frequency of rs1142345 and rs116855232 exhibits largely ethnic differences. According to the large genetic variant screening in diverse ethnicities, the risk allele frequency of rs1142345 is high in Caucasians (4%), Hispanics (4.8%), and Africans (5.4%) but low in East Asians (1.3%), while that of rs116855232 is high in East Asians (10.4%) and Hispanics (7.1%) but rare in Caucasians (0.46%) and Africans (0.07%) (http://exac.broadinstitute.org/). Therefore, concurrence of rs1142345 and rs116855232 risk allele (at least homozygous for one SNP) is pretty rare (Fig. 1c), which have never been reported in thiopurine treatment before. Indeed, the frequency of the genotype we reported is ~ 140.6 per million in East Asians. The variant can induce activity deficiency of both TPMT and NUDT15 enzymes, thus reducing the degradation of 6-thioguanine nucleotides through different mechanisms [1]. Not surprisingly, the patient suffered severe lifethreatening myelosuppression with very early occurrence and long duration after azathioprine treatment. Therefore, lower thiopurine dosage should be used in patients with such genotype than those with NUDT15 or TPMT risk /risk alone [2]. However, guidelines for autoimmune liver diseases have not referred to NUDT15 genetic examinations before azathioprine treatment. Given that NUDT15 and TPMT are commercially available to be checked, more clinicians should be aware that it is important to detect both TPMT and NUDT15 SNPs before thiopurine treatment in autoimmune liver diseases, especially in East Asians and Hispanics. * Li Yang yangli_hx@scu.edu.cn


Dear Editor,
A 46-year-old Chinese male patient was diagnosed with autoimmune hepatitis-primary biliary cholangitis overlap syndrome at the first admission on Apr 22nd 2016. He was treated with ursodeoxycholic acid, followed by prednisolone and azathioprine (Fig. 1a). He was admitted again with fever and sore throat on Sept 15th, showing visible throat swelling. Notably, a sharp drop of blood cells was observed, reaching the diagnostic criteria of grade 4 myelosuppression (Fig. 1a), with 0.18 × 10 9 /L for neutrophilic granulocytes. Azathioprine and prednisolone were stopped immediately, while human granulocyte colony-stimulating factor and recombinant human thrombopoietin were prescribed to increase blood cells. However, the patient still suffered a life-threatening progressive decrease of blood cells. The lowest level of neutrophilic granulocytes, reticulocytes, and white blood cells reached 0.01 × 10 9 /L, 0.0040 × 10 12 /L, 0.25 × 10 9 /L, respectively, on Sept 23rd, while the hemoglobin, platelets, and red blood cells dropped to the lowest level at 54 g/L, 22*10^9/L, and 1.66*10^1 2 /L, respectively, on Oct 1st (Fig. 1a). Moreover, diffuse rash and severe alopecia were observed. Serious infections (Pseudomonas aeruginosa infection) also occurred during hospitalization. The blood counts recovered far more slowly than expected and it returned to normal until 25 days (Oct 10th) after therapy (Fig. 1a). We attempted to genotype the SNPs of NUDT15 (rs116855232) and TPMT (rs1142345) of this patient and found that this patient has NUDT15 risk/risk TPMT wt /risk genotype (Fig. 1b).
The frequency of rs1142345 and rs116855232 exhibits largely ethnic differences. According to the large genetic variant screening in diverse ethnicities, the risk allele frequency of rs1142345 is high in Caucasians (4%), Hispanics (4.8%), and Africans (5.4%) but low in East Asians (1.3%), while that of rs116855232 is high in East Asians (10.4%) and Hispanics (7.1%) but rare in Caucasians (0.46%) and Africans (0.07%) (http://exac.broadinstitute.org/). Therefore, concurrence of rs1142345 and rs116855232 risk allele (at least homozygous for one SNP) is pretty rare (Fig. 1c), which have never been reported in thiopurine treatment before. Indeed, the frequency of the genotype we reported is~140.6 per million in East Asians. The variant can induce activity deficiency of both TPMT and NUDT15 enzymes, thus reducing the degradation of 6-thioguanine nucleotides through different mechanisms [1]. Not surprisingly, the patient suffered severe lifethreatening myelosuppression with very early occurrence and long duration after azathioprine treatment. Therefore, lower thiopurine dosage should be used in patients with such genotype than those with NUDT15 risk/risk or TPMT risk /risk alone [2]. However, guidelines for autoimmune liver diseases have not referred to NUDT15 genetic examinations before azathioprine treatment. Given that NUDT15 and TPMT are commercially available to be checked, more clinicians should be aware that it is important to detect both TPMT and NUDT15 SNPs before thiopurine treatment in autoimmune liver diseases, especially in East Asians and Hispanics.
Additionally, other less common SNPs in these two genes should be also considered, such as rs147390019 with an allele frequency of 1.7% in Hispanics [3].
Acknowledgements Thanks to our colleagues for their efforts and also acknowledge the cooperation from the patient.

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Conflict of interest The authors declare that they have no conflict of interest.