Efficacy of the Oral Administration of Maltodextrin Fructose Before Major Abdominal Surgery: A Prospective, Multicenter Clinical Study

Background To study the efficacy of the oral administration of maltodextrin and fructose before major abdominal surgery (MAS). Methods This prospective, multicenter, parallel-controlled, double-blind study included patients aged 45–70 years who underwent elective gastrectomy, colorectal resection, or duodenopancreatectomy. The intervention group (IG) was given 800 mL and 400 mL of a maltodextrin and fructose beverage at 10 h and 2 h before MAS, respectively, and the control group (CG) received water under the same experimental conditions. The primary endpoint was insulin resistance index (IRI), and the secondary endpoints were fasting blood glucose, fasting insulin, insulin secretion index, insulin sensitivity index, intraoperative blood glucose, subjective comfort score, and clinical outcome indicators. Results A total of 240 cases were screened, of which 231 cases were randomly divided into two groups: 114 in the IG and 117 in the CG. No time-treatment effect was detected for any endpoint. The IRI and fasting insulin were significantly lower in the IG than CG after MAS (p = 0.02 & P = 0.03). The scores for anxiety, appetite, and nausea were significantly lower in the IG than CG at 1 h before MAS. Compared with baseline, the scores for appetite and nausea decreased in the IG but increased in the CG. Conclusion The oral administration of maltodextrin and fructose before MAS can improve preoperative subjective well-being and reduce postoperative insulin resistance without increasing the risk of gastrointestinal discomfort.


Introduction
Insulin resistance (IR) is an important feature of the postoperative metabolic response. IR can decrease glucose uptake and utilization and increase endogenous glucose produced by gluconeogenesis in skeletal muscle and fat tissue [1,2]. As a key process in the pathophysiology of surgical stress, perioperative IR can result in postoperative hyperglycemia, which has been associated with a 30% increase in the risk of postoperative infection [3]. Moreover, IR is linked to delayed recovery and increased incidence of mortality and major complications [4].
Perioperative oral carbohydrate (OCH) loading is a well-established strategy to reduce surgical stress and modulate insulin sensitivity during and after surgery. Our previous study showed that IR after colorectal cancer surgery was significantly higher in the fasting and placebo groups than in the OCH group [5]. A systematic review demonstrated that there was a significant reduction in IR following preoperative OCH loading. The maximum increase in the effect of insulin after carbohydrate ingestion on the morning of surgery was 50% [6].
Preoperative OCH loading is an important item in the Enhanced Recovery After Surgery (ERAS) protocol. A Cochrane review found that OCH significantly reduced time to flatus by 0.39 days (95% CI: 0.70-0.07) and length of hospital stay (LOS) by 0.30 days (95% CI: 0.56-0.04) compared with fasting or placebo [7]. Another meta-analysis showed that, in patients undergoing major abdominal surgery (MAS), preoperative OCH loading reduced the LOS by 1.08 days (95% CI: 1.87-0.29) [8]. OCH loading is recommended by several ERAS guidelines, including the ASA Committee, ERAS Society, and ESPEN [9][10][11].
The concept of preoperative OCH loading has been widely accepted across China in the past few years. this study, including 120 patients from the intervention group (IG) and 120 patients from the control group (CG). All patients fasted for 6 h before MAS. The IG was given 800 mL and 400 mL of a 12.5% maltodextrin/fructose mixture solution (Suqian, Jiangsu Chia Tai Fenghai Pharmaceutical Co., Ltd.) at 10 h and 2 h before operation, respectively, and the CG received the same volume of water under the same experimental conditions. All patients were received multimodal analgesia with temperature monitoring. The patients were encouraged to mobilize as soon as possible after operation. No glucose solution was infused intraoperatively in both groups. After surgery, glucose was administered at a dose of 2 g/kg of body weight at a flow rate of 60 drops per minute, and the daily volume was limited to 1500-2000 mL. 3. Study design: Randomization was stratified by research center. The study was parallel-controlled (allocation ratio of 1:1) and double-blind. The maltodextrin and fructose beverage bottle or water was assigned to patients using random numbers generated by SAS software version 9.3.1. 4. Primary endpoint: homeostasis model assessment insulin resistance index (HOMA-IRI) was calculated as fasting blood glucose level (mmol/L) 9 fasting insulin level (mIU/L)/22.5 and was measured before randomization (baseline) and on days 1 and 3 after MAS. 5. Secondary endpoints: (1) fasting blood glucose (mmol/ L); (2) fasting insulin (lU/mL); (3) insulin secretion index (HOMA-b), calculated as 20 9 fasting insulin level/(fasting blood glucose level-3.5); (4) insulin sensitivity index (HOMA-ISI), calculated as 1/(fasting blood glucose level 9 fasting insulin level). These four parameters were measured at baseline (before randomization) and on days 1 and 3 after MAS; (5) subjective well-being (anxiety, thirst, appetite, nausea, and fatigue) measured using a visual analog scale at baseline (before randomization) and 1 h before MAS. The following scoring system was used to assess the level of discomfort: 0, none; 1-3, mild; 4-6, moderate; 7-9, severe; 10, intense; (6) blood glucose measured at 30, 60, 120, and 180 min after the start of surgery; (7) clinical outcomes, including infectious and non-infectious complications, postoperative flatus time, hospitalization time, and incidence of pulmonary aspiration during anesthesia. 6. Data input and statistical analysis: One data administrator developed the data input system, and two administrators independently entered and reviewed data to ensure accuracy. Quantitative data were described using means and standard deviations. A two-way repeated measures ANOVA was performed to evaluate the effect of different interventions over time on primary and secondary endpoints mentioned above. For significant two-way interactions (time and interventions), we analyzed the effect of intervention on endpoints at every time point using ANOVA tests. Bonferroni correction for p value was used as appropriate. Dichotomous data were described as frequencies, and intergroup differences were analyzed using a v2 test or Fisher exact test. Statistical analyses were performed using SAS software version 9.3.1. A twotailed p value of less than 0.05 was considered significant.

Patient demographics and baseline characteristics:
A total of 240 patients were screened, nine patients were excluded, and 231 patients were randomized (114 in the IG and 117 in the CG). Eight patients did not complete the study ( Fig. 1) because of changes in surgical procedure (seven cases) or cancellation of surgery (one case). Demographics, comorbidities, type of surgery, and surgical status are shown in Table 1 2. HOMA-IRI: Table 2 showed the descriptive and test statistics of HOMA-IRI at each time point for both On the other hand, there was a significant difference in HOMA-IRI between the IG and CG (group effect, p = 0.02), indicating that although the two groups showed similar trends over time, the CG showed significantly higher HOMA-IRI than the IG postoperatively. Subgroup analysis showed that there were significant group differences and time effects in HOMA-IRI in gastric and duodenopancreatectomy surgery patients. However, no significant differences were observed in terms of group effects within those who underwent colorectal and uncertain surgeries. 3. Secondary endpoints: Two-way repeated measures ANOVA results showed significant group effect (p = 0.01) and time effect (p \ 0.001) but non-significant interactions of time and intervention (p = 0.65) in intraoperative blood glucose 30, 60, 120, and 180 min after the start of MAS (Table 3). Similarly, no significant time-effect interactions were detected for other secondary ending points, according to two-way repeated measures ANOVA (Table 4). Time effects are significant for fasting blood glucose, insulin secretion index, and insulin sensitivity index, while group effect is significant for fasting insulin only, indicating CG had significantly higher fasting insulin than the IG postoperatively (group effect, p = 0.03). 4. Subjective well-being: Group effects were significant in anxiety, appetite, and nausea, while time effect was only significant in thirst score. Specifically, the intervention group had significantly lower anxiety score both at baseline and preoperative period, but significantly higher appetite and nausea scores were only found at preoperative period in control group. Effects of interventions on subjective well-being scores were also not time-dependent, according to two-way repeated measures ANOVA results (Table 5). 5. Outcome indicators: The rate of infectious and noninfectious complications was non-significantly lower in the IG than in the CG (Table 6). Postoperative flatus time and hospitalization time were similar between the two groups (Table 7). There were no cases of pulmonary aspiration during anesthesia in our sample.

Discussion
The oral administration of 800 mL and 200 mL of a carbohydrate solution at 10 and 2 h before MAS conformed to ERAS protocols. Evening doses increase hepatic glycogen storage, and a subsequent morning dose changes patient status from fasted to fed, potentially reducing IR [12]. Previous studies showed that the level of compliance with this protocol was high among CRS patients [13,14]. In this study, all participants completed this protocol, proving its feasibility in clinical practice. The carbohydrate concentration should be adjusted to maximize its effect on IR. In this respect, low-concentration glucose solutions, including 5% dextrose and 6-7% carbohydrate, do not have a significant metabolic effect by failing to stimulate insulin release. The dose used in this study-50 g of complex carbohydrates in 400 mL of fluid (equivalent to 12.5% carbohydrate)-decreases insulin resistance by 50% and has adequate osmolality for gastric emptying [14].
As reported in other studies, HOMA-IRI and fasting insulin were significantly lower in the IG than in the CG postoperatively, suggesting that preoperative OCH loading might increase insulin sensitivity, allowing reducing the amount of insulin to control blood sugar after surgery. However, there was no detectable effect of OCH loading on fasting blood glucose, HOMA-b and HOMA-ISI in the postoperative period. Subgroup analysis indicated that the effect of OCH loading differed among types of surgeries. In gastric and duodenopancreatectomy surgery patients, the alteration of HOMA-IRI was similar to that in all surgery. However, OCH loading had no detectable group or time effects on HOMA-IRI in patients who underwent CRS and uncertain surgery. The variable effect of OCH loading may be associated with the surgical site and degree of surgical stress. However, no time-treatment effect was detected in any outcome measurement in our study.
A major concern to anesthetists is the ingestion of 400 mL of a carbohydrate drink in the immediate preoperative period and the perceived elevated risk of pulmonary aspiration. A scintigraphic study employing a gamma camera showed that the gastric emptying of 400 mL of a 12.5% carbohydrate drink was achieved within 90 min in preoperative patients and healthy volunteers. Before anesthetic induction, there was no significant difference in residual gastric volumes between an intervention group receiving a 12.5% carbohydrate beverage and the placebo group [15]. Moreover, Kaska et al. showed that preoperative OCH loading did not reduce gastric emptying Test statistics for two-way repeated measure ANOVA are represented by F statistics (p value). The p value is interpreted as the probability against the hypothesis of no statistical differences across different intervention/time groups or intervention-time interaction as appropriate *P \ 0.05 as significant compared with fasting [16]. Our results corroborated the safety of this treatment because there were no cases of pulmonary aspiration during anesthesia in our sample. Several studies reported that carbohydrate beverages improved perioperative well-being. Hausel et al. found that the IG was less hungry and less anxious than both the fasting and placebo groups in the presurgical period [17]. A meta-analysis showed that a carbohydrate beverage significantly reduced thirst, appetite, anxiety, and discomfort compared with fasting and placebo (flavored water) [6]. In contrast, the Cochrane Review found no significant difference in patient well-being postoperatively between the intervention and fasting groups [18]. Our previous study showed that OCH loading reduced appetite compared with fasting 1 h before surgery. In this cohort, the effect of carbohydrate was similar to that reported previously. Furthermore, OCH loading attenuated nausea preoperatively.
The effects of carbohydrate relative to placebo on clinical endpoints, such as postoperative complications and LOS, remain controversial. A randomized controlled trial on MAS found that hospital stay was shorter in the carbohydrate group [15]. A meta-analysis of 21 randomized clinical trials showed that preoperative carbohydrate treatment significantly reduced the length of hospital stay by 1.08 days (95% CI: 1.87-0.29) in patients undergoing MAS [19]. In contrast, a network meta-analysis did not support this conclusion, and OCH loading before elective surgery slightly decreased the length of postoperative hospital stay compared with fasting and had no benefit over water or placebo. Moreover, the rates of postoperative complications or secondary outcomes were similar between the intervention and placebo groups [7]. A PROCY study reported that preoperative OCH loading maintained blood glucose levels to \ 180 mg/dL but did not reduce the risk of postoperative infectious complications compared with placebo in elective MAS (relative risk, 1.019; 95% confidence interval, 0.720-1.442, P = 1.00) [20]. In this study, we focused on MAS and, although the infection rate was lower and the LOS was shorter in the IG than in the CG, these differences were not significant. This result may be because ERAS measures have been used more often in the past few years and reduce the effect of OCH on postoperative clinical outcomes.
This study has several limitations. First, only patients aged 45-70 years with a BMI of 16.7-31.6 were enrolled, and those with severe metabolic diseases were not included. Second, the study is multicenter, and tests were performed in six biochemical laboratories, which might affect the consistency of the results. Third, the non-standardization of the evening meal consumed the day before the study might affect our data. Fourth, the null-significance in subgroup analysis could also subject to lower power due to the small sample sizes.  Test statistics for two-way repeated measure ANOVA are represented by F statistics (p value). The p value is interpreted as the probability against the hypothesis of no statistical differences across different intervention/time groups or intervention-time interaction as appropriate *P \ 0.05 as significant Test statistics for two-way repeated measure ANOVA are represented by F statistics (p value). The p value is interpreted as the probability against the hypothesis of no statistical differences across different intervention/time groups or intervention-time interaction as appropriate c Baseline refers to subjective well-being before randomization; preoperative refers to subjective well-being at 1 h before surgery * P \ 0.05 as significant Statistical analysis used: Differences between the two groups were analyzed using v2 test In conclusion, the oral administration of maltodextrin and fructose before MAS reduced IR in the postoperative period and improved subjective comfort preoperatively. Moreover, this treatment did not cause pulmonary aspiration during anesthesia, demonstrating its safety in clinical practice.