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Changes of Facial Lipidomics by Intense Pulsed Light Treatment Based on LC-MS

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  • Non-Surgical Aesthetic
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Abstract

Background

Intense pulsed light (IPL) has been widely used to improve cutaneous photoaging in recent years. Several studies began to explore the changes of skin barrier function after treatment, but the changes of skin surface lipids (SSL), especially specific lipid content and types are still unclear.

Methods

A total of 25 female volunteers were included in our study, and each of them received three full-face treatments with one month apart. Before the first treatment and 1 month after the last treatment, we collected clinical photos and skin stratum corneum samples from individuals. A 5-level scale was used to evaluate the efficacy of IPL treatment, liquid chromatography-mass spectrometry (LC-MS), and Orthogonal Partial Least Squares Discrimination Analysis (OPLS-DA) were used to analyze the changes of SSL.

Results

Two patients got no improvement after treatment, 6 patients had poor improvement and mild improvement was achieved in 9 patients, 5 and 3 patients reported moderate and significant improvement. The overall “effective” rate was 68 % and the “significant effective” rate was 32 %. The results showed 18 lipid subclasses and 487 lipid molecules were identified. The change of total lipid volume was not statistically significant (P = 0.088>0.05), but lipid subclass analysis showed the amount of Triglyceride (TG), Phosphatidic Acid (PA), Phosphatidylglycerol (PG) and Lysophosphatidylglycerol (LPG) were significantly increased (P < 0.05). There were 55 kinds of lipid molecules with significant difference after treatment (P < 0.05), and 51 of them belong to TG. The analysis of chain saturation of TG showed that the quantity of TG with 0, 1 and 2 unsaturated bonds increased significantly (P < 0.05).

Conclusions

IPL treatment does not have a significant effect on the overall amount of lipids while the amount of TG, PA, PG, LPG were significantly increased. These lipid changes may potentially improve the skin barrier function, but more high-quality and comprehensive studies are still needed.

Bullet Point

Lipidomics analysis based on LC-MS; Changes of skin surface lipid after IPL treatment; the relationships between skin surface lipid and skin barrier functions.

Level of Evidence IV

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Funding

This study was supported by Hangzhou health science and technology key project (No 20220054).

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Authors and Affiliations

  1. Department of Cosmetic Dermatology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Zhejiang Provincial Hospital of Chinese Medicine, Youdian Road 54, Hangzhou, 310009, People’s Republic of China

    YuJia Cai

  2. Department of Dermatology, Hangzhou Third People’s Hospital, Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University, West Lake Road 38, Hangzhou, 310009, People’s Republic of China

    WenZhong Xiang

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  1. YuJia Cai
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Correspondence to WenZhong Xiang.

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The authors declare that they have no conflict of interest to disclose.

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All procedures performed in studies involving humanparticipants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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All participants have given their informed consent in writing prior to inclusion in the study, any identifying details of the participants will not be published in written descriptions, photographs, and genetic profiles, and the participant has given written informed consent for publication.

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Cai, Y., Xiang, W. Changes of Facial Lipidomics by Intense Pulsed Light Treatment Based on LC-MS. Aesth Plast Surg 48, 2171–2178 (2024). https://doi.org/10.1007/s00266-023-03842-w

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  • DOI: https://doi.org/10.1007/s00266-023-03842-w

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