Pelvic lymph-node staging with 18F-DCFPyL PET/CT prior to extended pelvic lymph-node dissection in primary prostate cancer - the SALT trial -

Purpose The detection of lymph-node metastases (N1) with conventional imaging such as magnetic resonance imaging (MRI) and computed tomography (CT) is inadequate for primarily diagnosed prostate cancer (PCa). Prostate-specific membrane antigen (PSMA) PET/CT is successfully introduced for the staging of (biochemically) recurrent PCa. Besides the frequently used 68gallium-labelled PSMA tracers, 18fluorine-labelled PSMA tracers are available. This study examined the diagnostic accuracy of 18F-DCFPyL (PSMA) PET/CT for lymph-node staging in primary PCa. Methods This was a prospective, multicentre cohort study. Patients with primary PCa underwent 18F-DCFPyL PET/CT prior to robot-assisted radical prostatectomy (RARP) with extended pelvic lymph-node dissection (ePLND). Patients were included between October 2017 and January 2020. A Memorial Sloan Kettering Cancer Centre (MSKCC) nomogram risk probability of ≥ 8% of lymph-node metastases was set to perform ePLND. All images were reviewed by two experienced nuclear physicians, and were compared with post-operative histopathologic results. Results A total of 117 patients was analysed. Lymph-node metastases (N1) were histologically diagnosed in 17/117 patients (14.5%). The sensitivity, specificity, positive predictive value and negative predictive value for the 18F-DCFPyL PET/CT detection of pelvic lymph-node metastases on a patient level were 41.2% (confidence interval (CI): 19.4–66.5%), 94.0% (CI 86.9–97.5%), 53.8% (CI 26.1–79.6%) and 90.4% (CI 82.6–95.0%), respectively. Conclusion 18F-DCFPyL PET/CT showed a high specificity (94.4%), yet a limited sensitivity (41.2%) for the detection of pelvic lymph-node metastases in primary PCa. This implies that current PSMA PET/CT imaging cannot replace diagnostic ePLND. Further research is necessary to define the exact place of PSMA PET/CT imaging in the primary staging of PCa.


Introduction
Prostate cancer (PCa) is the most frequently diagnosed cancer in men in the Western world [1,2]. Initial therapy includes robot-assisted radical prostatectomy (RARP) and external beam radiation therapy. Accurate assessment of local tumour stage (T-stage), regional lymph-node involvement (N-stage) and screening for distant metastases (M-stage) is essential, since it significantly affects patient follow-up, therapeutic decisions and oncological outcome [3]. Conventional imaging studies such as computed tomography (CT) and magnetic resonance imaging (MRI) have moderate sensitivity for the detection of lymph-node metastases (42% and 39%, respectively) [4][5][6]. Therefore, extended pelvic lymph-node dissection (ePLND) remains the preferred technique for nodal staging. It is an invasive procedure, however, associated with complications such as lymphocele, deep venous thrombosis, and longer hospital stay [7].
Only few studies have evaluated the accuracy of PSMA PET/CT for nodal staging of primary PCa, comparing scan results with the histopathology of surgical ePLND specimens. In patients with intermediate and high-risk disease, 68 Ga-PSMA showed modest sensitivity for lymph-node metastatic disease, at consistently high specificity [16][17][18][19]. For 18 F-DCFPyL PET/CT, only a single small prospective series for staging newly diagnosed PCa has been published yet [20]. This is the first large, prospective study on the staging accuracy for the detection of PCa lymph-node metastases using 18 F-DCFPyL PET/CT imaging (acronym: SALT trial). The primary aim was to assess the accuracy to detect pelvic lymph-node metastases with 18 F-DCFPyL PET/CT, in patients with intermediate and high-risk PCa. As a secondary objective, the ability of 18 F-DCFPyL PET/CT to predict the pathological local tumour stage (pT) was assessed. The imaging results from 18 F-DCFPyL PET/CT were compared with final histopathology from the ePLND and radical prostatectomy.

Study design and patient population
This was a prospective, non-randomized study evaluating the accuracy of 18 F-DCFPyL PET/CT for detecting pelvic lymphnode metastases in patients with primary PCa undergoing radical surgery. Imaging results were compared with histopathology following RARP and ePLND (reference standard). The study has been approved by the ethical review board of the Amsterdam University Medical Centre (review number 2017.543), and was registered in the Netherlands Trial Register (NTR 6754). All subjects were included consecutively, and signed informed consent for collection of their clinical data and analysis of the 18 F-DCFPyL PET/CT scans, prior to RARP. Patients were enrolled between October 2017 and January 2020 in two reference centres of the Prostate Cancer Network Netherlands (Amsterdam University Medical Centre; Noordwest ziekenhuisgroep). Inclusion criteria were (1) histologically proven, intermediate or high-risk PCa [3] and (2) patients undergoing RARP and ePLND. Patients with distant metastases were not considered for evaluation, as no RARP and ePLND is performed in such cases. Of all included patients, age, prostate volume, initial PSA level, pathological biopsy features (histopathological grade, number of cores with cancer) and EAU risk category were collected [3]. The indication to perform ePLND was based on a ≥ 8% risk of lymphnode involvement as predicted by the Memorial Sloan Kettering Cancer Centre (MSKCC) nomogram [21], or on the presence of high-risk features: PSA > 20 ng/mL, Gleason score 8-10 or suspicion of cT2c or higher [3]. Patients with incomplete ePLND due to intraoperative difficulty in performing ePLND were excluded from final analysis. The required sample size was calculated at 120 patients, and was based on a 30% incidence of lymph-node metastases overall, with an estimated sensitivity of 90% (lower boundary of the 95% confidence interval (CI) at 80%) [22,23].

Image acquisition
Patients were staged with 18 F-DCFPyL PET/CT in the Amsterdam UMC or Noordwest ziekenhuisgroep. 18 F-DCFPyL was synthesized under good manufacturing practices conditions at both centres. PET images were made at a median of 118 min after injection (interquartile range [IQR] 112-123 min) of a median dose of 311 MBq 18 F-DCFPyL (IQR 297-324 MBq) within a median of 4.1 weeks (IQR 2.1-6.6) after prostate biopsy and within a median of 5.9 weeks (IQR 3.6-12.0) prior to surgery. Image acquisitions were performed using a Philips Ingenuity TF (Philips Healthcare®, the Netherlands/USA) and a Siemens Biograph-16 TruePoint (Siemens Healthineers®, Germany) PET/CT system. The scan trajectory included mid-thigh to skull base, with 4 min (Philips) and 5 min (Siemens) per bed position. All PET scans were combined with a low-dose CT (33/117 patients) or contrast-enhanced CT scan (84/117 patients) (30-110 mAs, 110-130 kV). Images were corrected for decay, scatter, random coincidences and photon attenuation.

Scan interpretation
All scans were clinically and prospectively interpreted in the participating centres by one of two nuclear medicine physicians (DO, MW) with ample experience in PSMA PET interpretation (> 300 scans). Upon completion of the study, all scans were reviewed by a second independent reader who was blinded to initial scan interpretation, surgery and histopathology results (DO, GZ). A joint re-evaluation was performed in case of incongruent scan interpretation (consensus read), and used for final analysis. Lymph-node metastases were defined as increased PSMA expression, higher than the background, incompatible with physiological uptake, and in a typical site of PCa. A significant CT substrate was not an absolute prerequisite. The following parameters were recorded: detection of the primary tumour, tumour stage and presence of pelvic lymph-node metastases (N1). Pelvic lymphnode metastases were further classified in accordance with the four sections of the ePLND (external iliac artery left/ right; obturator fossa left/right).

Inter-observer variability
To assess inter-observer variability, the proportional agreement was calculated, based on the two individual scan interpretations [25]. Proportional agreement was defined as the degree to which the two independent PET/CT readings were reported as the same. Positive agreement was defined as the degree to which the two independent PET/CT readings were reported both positively, whereas negative agreement was defined as the degree to which the two independent PET/CT readings were reported both negatively.

Semi-quantitative analysis
PET/CT scans with PSMA-avid lesions in the prostate were delineated according to the reports of the nuclear medicine physicians. Semi-automatic delineation on the PET scans from both participating centres was performed with the inh o u s e d e v e l o p e d A C C U R A T E t o o l © [ 2 6 ] . T h e ACCURATE tool semi-automatically generated a volumeof-interest using a 50% isocontour based on standard uptake value peak (SUV peak ), with correction for background uptake [26]. SUV peak is defined as the highest local intensity of uptake with a 6-mm-radius sphere [27]. To compare the median SUV peak of the prostate tumour for patients with and without lymph-node metastases, the Mann-Whitney U test was used (significance set at p < 0.05). To compare the median SUV peak of the prostate tumour with the corresponding Gleason score of the lesion, the Kruskall-Wallis test was used (significance set at p < 0.05). A linear regression was run to predict PSA from SUV peak of the prostate index lesion (significance set at p < 0.05).

Surgical procedure and histopathologic evaluation
The ePLND surgical template includes removal of fatty lymphoid tissue overlying the common and external iliac vessels and within the obturator fossa [3]. The medial border of the surgical template of ePLND concerns the ureter, the caudal border the obturator nerve and the cranial border the genitofemoral nerve continued to the inner aspect of the pubic bone. All four template sections were individually collected and presented for histopathologic analysis.

Histopathology of resected specimens
Prostate specimens and resected lymph-node templates were fixated in formaldehyde (10%) directly after surgery and processed according to routine clinical standards [3]. Individual lymph nodes were manually picked from the surgical specimens and sectioned (< 3 mm as a whole, 3-10 mm in half and > 10 mm in multiple) to make histologic slices. All slices were reviewed by dedicated uro-pathologists (unblinded from PET results), reporting tumour Gleason score, pathological tumour stage (pT) and total number of resected lymph nodes. The maximum diameter (mm) of metastatic nodal deposits was recorded (mm).

Outcome measurement and statistical analysis
The primary outcome of this study was the patient-based sensitivity of 18 F-DCFPyL PET/CT to detect pelvic lymph-node metastases. The diagnostic accuracy of 18 F-DCFPyL PET/CT was calculated with histopathologic evaluation of ePLND as a reference. The sensitivity, specificity and positive and negative predictive values (PPV and NPV, respectively) of 18 F-DCFPyL PET/CT for the detection of pelvic lymph-node metastases (pN1) were calculated both on a patient level and on a surgical template level. The surgical template analysis was based on the abovementioned 4 surgical templates of the ePLND, and was applied to approximate lesion baseddetection using 18 F-DCFPyL PET/CT. To compare the median diameter of PET/CT-detected lymph-node metastases vs. PET/CT-undetected lymph-node metastases, the Mann-Whitney U test was used (significance set at p < 0.05).
For the assessment for the local tumour stage (pT), we measured the accuracy of 18 F-DCFPyL PET/CT to differentiate local advancement (T3 a-b, T4) from prostate-confined disease (T2). This study did not investigate the exact location of local PCa advancement, only the presence of extracapsular or seminal vesicle invasion was noted. Local advancement was defined as PSMA expression outside the borders of the prostate gland, not suspect for overprojection or bladder/urethral physiological activity. Numerical variables were summarized with median values and interquartile ranges (IQR), categorical variables with proportions (%). Statistical analysis was done with IBM® SPSS® Statistics for Windows®, version 26.

Patient characteristics
A total of 120 patients were included in this study, and scheduled for ePLND with RALP after 18 F-DCFPyL PET/CT, as presented in Fig. 1. Three patients were excluded from the final analysis: one patient ultimately proved to be unfit for surgery, and the second was excluded because surgery was not completed due to intraoperative complications (intestinal perforation). A third patient revoked his informed consent during the study period, after initial consent. One patient did not receive a complete prostate removal due to persistent intraoperative bleeding, which made the surgeon decide not to continue surgical resection of the prostate. The preceding ePLND was completed, however, and the patient was included for analysis of lymph-node metastases. Therefore, a total of 117 patients were included for final analysis for the accuracy of N-staging, and 116 patients for the accuracy of T-staging. Included patients had a median age of 67 years (IQR 61-70), and a median initial PSA level of 10

Accuracy of 18 F-DCFPyL PET/CT for detecting pelvic lymph-node metastases
Pathological features after RARP and ePLND are listed in Table 2. A total of 2149 lymph nodes were resected during surgery (median 18 lymph nodes per patient, . In 17/117 patients, lymph-node metastases were diagnosed (14.5% of total). Of the 17 patients with lymphnode metastases on histopathological evaluation, 7 patients had a 18 F-DCFPyL PET/CT suspicious for lymphnode metastases. Hence, the patient-based sensitivity to detect lymph-node metastases using 18 Table 3.
In the 17 patients with lymph-node metastases, 31 lymphnode metastases were histologically identified in 23 surgical ePLND templates. 18 F-DCFPyL PET/CT preoperatively identified 38 PSMA-avid regions suspect for lymph-node metastases in 18 surgical ePLND templates. Therefore, the template-based sensitivity for the detection of lymph-node metastases using 18 Table 3. For a detailed overview of false-positive and negative lymph nodes that were misdiagnosed by 18 F-DCFPyL PET/CT, please see Fig. 2.
The median diameter of resected lymph-node metastases was 2.5 mm (IQR 1.0-6.0). The PET/CT-detected lymphnode metastases (n = 12) had a median tumour size of 5.5 mm (IQR 2.4-6.6), whereas the PET/CT-undetected lymph-node metastases (n = 19) had a significantly smaller median tumour size of 1.5 mm (IQR 1.0-4.5) (p = 0.03). A clinical example of a patient with both missed and detected lymph-node metastases is shown in Fig. 3. A clinical example of a patient with a false-positive lymph-node metastasis is shown in Fig. 4.

Local staging
A total of 116/117 patients (99.1%) showed PSMA expression in the prostate at PET/CT. The sensitivity, specificity, PPV and NPV of 18 Fig. 5.
No significant difference in the median SUV peak of the PSMA-avid prostate lesions was found between patients with and without lymph-node metastases (SUV peak = 6.4, IQR 3.8-10.9, vs. 6.7, IQR 5.2-18.6) (p = 0.46). A Gleason score of 4 + 5 = 9 or higher was associated with a higher median SUV peak when compared with the lower Gleason scores (5.8 vs. 15.0, p = 0.02). A Gleason score of 4 + 4 = 9 or higher was not associated with a higher median SUV peak when compared with the lower scores (5.6 vs. 9.4, p = 0.09). No correlation was found between SUV peak of the PSMA-avid prostate lesions and PSA (R 2 = 0.12).

Discussion
PSMA PET/CT imaging is currently the imaging technique of choice for patients with biochemically recurrent disease after initial curative local treatment (EAU guidelines) [28]. Its value for staging of primary PCa is less established, however. This the first large prospective analysis using 18 F-DCFPyL PET/ CT for primary staging of PCa, assessing the diagnostic accuracy for the detection of pelvic lymph-node metastases. The results of a total of 117 patients with intermediate and highrisk PCa that underwent 18 F-DCFPyL PET/CT and ePLND were analysed.
In this study, 18 F-DCFPyL PET/CT imaging demonstrated a limited sensitivity for pelvic lymph-node metastases of 41.2%, at 94.0% specificity. The limited sensitivity indicates that 18 F-DCFPyL PET/CT does not detect all lymph-node metastases. Therefore, albeit invasive, ePLND remains the gold standard for nodal staging. The 90% NPV might suggest that a negative test is reliable in the majority of times, though we should mind the low prevalence of lymph-node metastases in this cohort (14.5%). This prevalence was congruent with the median MSKCC risk for lymph-node metastases of 14.4% (IQR 10.1-30.2). Although specificity for lymphnode metastases was favourable in this study, the PPV was moderate at 53.8% (due to the low prevalence). In this clinical setting, not all positive 18 F-DFCPyL PET/ CT results for pelvic lymph-node metastases represent actual metastatic disease. Good intra-observer agreement for the detection of pelvic lymph-node metastases using 18 F-DFCPyL PET/CT was observed (95%). Our results appear to be in line with a recent prospective 68 Ga-PSMA study from van Kalmthout et al. [16]. This study used a similar methodology to our study (n = 103 patients), applying the same standardized ePLND techniques, histopathology analyses and PET positivity criteria. It revealed a patient-based sensitivity for lymphnode metastases of 41.5% (95%CI 26.7-57.8) and a specificity of 90.9% (95%CI 79.3-96.6) [16]. Although accuracy was similar, we should note that the prevalence of lymph-node metastases in their study was much higher (42.3% vs. our 14.5%). This is likely due to the higher proportion of patients with high-risk disease in the van Kalmthout et al. study (89.3% vs. our 63.3%). This strengthens the notion that 18 F-DCFPyL PET/CT is at least comparable with 68 Ga-PSMA imaging.

Included paƟents with indicaƟon for extended pelvic lymph node dissecƟon (ePLND) that received a 18 F-DCFPyL PET/ CT and provided wriƩen informed consent(N=120
The high specificity presented in the current study confirms results from previous retrospective studies with 68 Ga PSMA ligands, which reported a specificity of 90% and higher [6,18,19,29,30]. Only one prospective study reported on 18 F-DCFPyL PET/CT as an imaging tool for initial staging of PCa [20]. In 25 patients with high-risk PCa, Gorin et al. reported a patient-based sensitivity for lymph-node metastases of 71.4% (95%CI 29.0-96.3), with a specificity of 88.9% (95%CI 65.3-98.6), at a prevalence of 28% [20]. Potentially, this higher sensitivity for lymph-node metastases is explained by the inclusion of more patients with high-risk disease (100.0% vs. our 63.3%), bearing higher PSMA expressing metastases [9].    ISUP 5 = Gleason score 4 + 5 = 9/Gleason score 5 + 4 = 9/Gleason score 5 + 5 = 10 b In one patient, extended lymph-node dissection was successfully performed, yet surgical removal of the prostate proved unfeasible due to extensive intraoperative bleeding PET/CT-detected lymph-node metastases were larger than lymph-node metastases that were not detected by PET/CT (median 5.5 mm vs. 1.5 mm, Mann-Whitney U test: p = 0.03). This may explain the imperfect imaging sensitivity reported in this study. The 5-mm spatial resolution offered by PET is still an improvement compared with the detection limits of CT and MRI (i.e. > 10 mm [4]). This discrepancy in spatial resolution could explain why PSMA PET/CT is repeatedly found to be more sensitive than conventional imaging, as confirmed by a recent meta-analysis by Wu et al. [6]. This study reported on a difference in sensitivity for the detection of lymph-node metastases of 68 Ga-PSMA PET/CT vs. MRI (65% vs. 41%) [6].
The therapeutic consequence of PSMA-detected pelvic lymph-node metastases remains a matter of debate. Previous research showed that patients with lymph-node metastases detected intra-operatively (with frozen sections) or preoperatively (with CT) still benefit from radical prostatectomy and complete lymph-node dissection [31][32][33][34]. This suggests that detection of (a limited number of) pelvic lymph-node metastases with 18 F-DCFPyL PET/CT should still be followed by curative treatment in the form of a RALP with an ePLND.   However, the threshold to perform ePLND with certain amounts of detected number of lymph-node metastases remains unclear.
A total of 116/117 patients (99.1%) showed PSMA expression in the prostate at PET/CT. A promising PPV for the detection of pT3a-b of 90.3% (95%CI 73.1-97.5) was observed using 18 F-DCFPyL PET/CT, yet the sensitivity was limited at 45.2% (95%CI 32.7-58.2). Moreover, the promising specificity of the detection of pT3a-b using 18 F-DCFPyL PET/CT of 94.4% (95%CI 83.7-98.6) is in line with previous reports on 68 Ga-PSMA (specificity > 90% for T3b) [29,35,36]. The high specificity for the detection of pT3a-b PCa 18 F-DCFPyL PET/CT is comparable with that of mpMRI, for which a meta-analysis revealed a specificity of 88% (95%CI 85-97%) compared with our 94.4% (95%CI 83.7-98.6) [37]. The sensitivity, however, was better for mpMRI at 0.61 (95%CI 0.54-0.67) vs. our sensitivity of 45.2% (95%CI 32.7-58.2) [37]. Altogether, it seems that current PSMA PET/CT does not outperform mpMRI for the detection of extra-prostatic growth of PCa. Clinically, the distinction between organ confined (T2) and extra-prostatic growth (T3) is of therapeutic importance (i.e. for planning nerve sparing surgery) [3]. We recommend nuclear medicine physicians to report on the absence or presence of tracer uptake suspect for extra-prostatic growth specifically. The lower positive agreement score (56%), however, may indicate that dual reading is advisable for routine clinical care as well.
Our study has inherent limitations. Firstly, this study did not assess the accuracy of 18 F-DCFPyL PET/CT for detecting distant metastases. Only patients undergoing RARP and ePLND were considered for analysis, which naturally excludes patients with distant metastases in which radical surgery is forgone. As such, our results should be interpreted as the accuracy of 18 F-DCFPyL PET/CT for N-staging in patients (expected to be) free from distant metastases (i.e. eligible for radical treatment). Therefore, we decided to focus our study on determining the accuracy of 18 F-DCFPyL PET/CT for N-staging, as hereto a solid reference standard is available (ePLND). Determining the accuracy of M-staging is certainly of interest, yet any such analysis is limited to providing a PPV, as the true prevalence of distant metastases cannot be known.
Since the PET/CT resolution is confined at 5 mm, limited diagnostic accuracy for micro metastases is to be expected. Secondly, this study might not have been adequately powered, since the expected prevalence was higher than the actual prevalence (30% vs. 14.5%). Moreover, the sensitivity used for the power analysis was higher than actually realized (90% vs. 41.2%) due to the high expectations for the sensitivity of PSMA PET/CT. Lastly, we should consider that the golden standard (ePLND) is not always flawless: in two patients with Fig. 3 A 68-year-old man with cT2c, Gleason score 3 + 4 = 7 prostate cancer and initial PSA 10.4 ng/mL considered candidate for radical prostatectomy with extended pelvic lymph-node dissection. MSKCC nomogram showed 10.8% risk of lymph-node involvement. Transversal 18 F-DCFPyL PET (a) and fused PET/CT (b) show intense uptake in the pelvic region right, corresponding with an enlarged 10-mm lymph node adjacent to the right external iliac artery on CT (c), suspect for lymphnode metastasis (a-c, left arrow). A contralateral focus with faint uptake is observed on PET and fused PET/CT in the pelvic region, without an evident morphologic substrate on CT. Due to the minimal tracer uptake (above the blood pool and lower than the liver); this left-sided focus was not suspect for lymph-node metastasis after dual reading. After surgical resection of 26 lymph nodes, post-operative histopathology revealed a right-sided right iliac lymph-node metastasis measuring 10 mm, as well as a left iliac lymph-node metastasis of 5 mm, haematoxylin and eosin stain, original magnification × 10 (d) PET-positive lymph nodes, the ePLND was reported to be technically challenging. Histopathological analysis did not reveal any lymph-node metastases, yet these patients soon developed a biochemical recurrence. Repeated 18 F-DCFPyL PET images were obtained, again detecting positive lymph nodes in the surgical template. Metastasis-directed radiotherapy to these lesions was followed by a PSA-response. The ePLND may possibly have missed these lesions initially that were rightfully detected on the first PET/CT scan. The follow-up data of this cohort is necessary to investigate whether a specific risk profile in combination with a negative 18 F-DCFPyL PET/CT scan could be used to withhold PCa patients from an ePLND. Future studies are therefore needed to assess whether the diagnostic accuracy of 18 F-DCFPyL PET/CT, its high specificity in particular, could assist in proper treatment planning of patients with intermediate and highrisk stages of disease.

Conclusion
In this prospective cohort study, we evaluated the accuracy of 18 F-DCFPyL PET/CT imaging for the detection of lymphnode metastatic disease in men with intermediate and highrisk prostate cancer, undergoing radical surgery. We found a limited sensitivity of 41.2% (95%CI 19.4-66.5) at excellent specificity (94.0%). Based on current results, 18 F-DCFPyL PET/CT imaging should not replace ePLND.
Funding Information Open access funding provided by Amsterdam UMC (Vrije Universiteit Amsterdam).
Data availability Data are available on request to the corresponding author.

Compliance with ethical standards
Competing interests Prof. Dr. Boellaard reports the receiving of a grant from Philips Healthcare, outside the submitted work. The other authors declare that they have no competing interests. Histopathological analysis showed a bilateral pT3b, Gleason score 4 + 5 = 9 PCa in the radical prostatectomy specimen, haematoxylin and eosin stain, original magnification × 5 (d). No lymph-node metastases were found after histopathological analysis of 15 resected lymph nodes Ethical approval This study was approved by the institutional research board of the Amsterdam UMC (location VUmc), and each participant provided informed consent to participate.

Consent for publication Each participant provided consent for data publication.
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