Abstract
The plant-associated microbiome has an effect on plant growth. Pulsatilla chinensis (Bge.) Regel is an important Chinese medicinal plant. Currently, there is little understanding of the P. chinensis-associated microbiome and its diversity and composition. Here, the core microbiome associated with the root, leaf, and rhizospheric soil compartments of P. chinensis from five geographical locations was analyzed by the metagenomics approach. The alpha and beta diversity analysis showed that the microbiome associated with P. chinensis was shaped by the compartment, especially in the bacterial community. The geographical location had little influence on microbial community diversity associated with root and leaf. Hierarchical clustering distinguished the microbial communities of rhizospheric soil based on their geographical location and among the soil properties, pH was showed the more stronger effect on the diversity of rhizospheric soil microbial communities. Proteobacteria was the most dominant bacterial phylum in the root, leaf, and rhizospheric soil. Ascomycota and Basidiomycota were the most dominant fungal phyla in different compartments. Rhizobacter, Anoxybacillus, and IMCC26256 were the most important marker bacterial species for root, leaf, and rhizospheric soil screened by random forest, respectively. The fungal marker species for root, leaf, and rhizospheric soil were not only different across the compartments but also the geographical locations. Functional analysis showed that P. chinensis-associated microbiome had the similar function which had no obvious relationship with geographical location and compartment. The associated microbiome indicated in this study can be used for identifying microorganisms related to the quality and growth of P. chinensis.
Key points
• Microbiome associated with P. chinensis was shaped by the compartment
• Microbiome composition and abundance associated with rhizospheric soil were affected by the geographical location
• Compared with fungi, bacterial associated with P. chinensis composition and diversity were more stable in different geographical locations and compartments
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Data availability
The datasets generated during and/or analyzed during the current study are available in the NCBI repository (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA907799; https://www.ncbi.nlm.nih.gov/bioproject/PRJNA907757).
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Funding
This work was supported by the 2019 Liaoning Provincial Department of Education Scientific Research Project, China (No. L201942); Liaoning Bai Qian Wan Talents Program; Liaoning Revitalization Talents Program (No. XLYC2002004) and Natural Science Foundation of Liaoning Province (No. 2020-MS-224).
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LX and QC. Conceptualization, methodology. CB. Writing—review and editing. YS and HX. Resources. WM. Visualization. YX. Data curation, writing—original draft preparation. WH and YY. Software, validation. Authors consent to participate. Authors consent for publication.
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Xing, Y., Bian, C., Xue, H. et al. The effect of plant compartment and geographical location on shaping microbiome of Pulsatilla chinensis. Appl Microbiol Biotechnol 107, 5555–5567 (2023). https://doi.org/10.1007/s00253-023-12641-x
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DOI: https://doi.org/10.1007/s00253-023-12641-x