Abstract
Santalenes and santalols from Santalum album are the main components of the valuable spice sandalwood essential oil, which also has excellent pharmacological activities such as antibacterial, anti-inflammatory, and antitumor. Firstly, we constructed biosynthesis pathways of santalenes by synthetic biology strategy. The assembled biosynthetic cassettes were integrated into the multiple copy loci of δ gene in S. cerevisiae BY4742 with assistance of pDi-CRISPR, and 94.6 mg/L santalenes was obtained by shake flask fermentation of engineered yeast. Secondly, a selected optimized P450-CPR redox system was integrated into the chromosome of the santalenes-producing strain with a single copy, and 24.6 mg/L santalols were obtained. Finally, the yields of santalenes and santalols were increased to 164.7 and 68.8 mg/L, respectively, by downregulating ERG9 gene. This is the first report on the de novo synthesis of santalols by P450-CPR chimera in S. cerevisiae. Meanwhile, the optimized chimeric CYP736A167opt-46tATR1opt exhibits higher activity to oxidize santalenes into santalols. It would provide a feasible solution for the optimal biosynthesis of santalols.
Key points
• First-time de novo synthesis of santalols by P450-CPR chimera in S. cerevisiae.
• Truncated 46tATR1 has higher activity than that of CPR2.
• Yields of santalenes and santalols were increased by downregulating ERG9 gene.
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Data availability
The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.
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This work was supported by the National Natural Science Fund 31760189, Yunnan Bao Shan 7th Batch Technological Innovation Talents Project 201805.
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W.M.L, H.X.L., and W.Y.C. conceived and designed the study. G.X.W did the gas chromatography mass spectrometry measurements. W.Y.C., L.F., and Z.S.S. conducted experiments in lab. L.M.G. and Z.J.Y. analyzed the data. W.Y.C. wrote the initial manuscript. H.X.L. and W.M.L. edited the manuscript. All authors read and approved the manuscript.
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Wang, Y., Gong, X., Li, F. et al. Optimized biosynthesis of santalenes and santalols in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 105, 8795–8804 (2021). https://doi.org/10.1007/s00253-021-11661-9
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DOI: https://doi.org/10.1007/s00253-021-11661-9