Evaluation of the radioactive contamination in fungi genus Boletus in the region of Europe and Yunnan Province in China

Numerous species of wild-grown mushrooms are among the most vulnerable organisms for contamination with radiocesium released from a radioactive fallout. A comparison was made on radiocesium as well as the natural gamma ray-emitting radionuclide (40K) activity concentrations in the fruiting bodies of several valued edible Boletus mushrooms collected from the region of Europe and Yunnan Province in China. Data available for the first time for Boletus edulis collected in Yunnan, China, showed a very weak contamination with 137Cs. Radiocesium concentration activity of B. edulis samples that were collected between 2011 and 2014 in Yunnan ranged from 5.2 ± 1.7 to 10 ± 1 Bq kg−1 dry matter for caps and from 4.7 ± 1.3 to 5.5 ± 1.0 Bq kg−1 dry matter for stipes. The mushrooms Boletus badius, B. edulis, Boletus impolitus, Boletus luridus, Boletus pinophilus, and Boletus reticulatus collected from the European locations between 1995 and 2010 showed two to four orders of magnitude greater radioactivity from 137Cs compared to B. edulis from Yunnan. The nuclide 40K in B. badius was equally distributed between the caps and stipes, while for B. edulis, B. impolitus, B. luridus, B. pinophilus, and B. reticulatus, the caps were richer, and for each mushroom, activity concentration seemed to be more or less species-specific.


Introduction
The nuclear accident in Chernobyl, which took place on 26th of April 1986 caused large-scale diffusion of radioactivity mostly in the Central and Northern Europe, but it was detected also in other southern areas in Turkey (IAEA 2005;Simsek et al. 2014). Because of that accident, the long-term residual radioactivity in the affected areas comes largely from radiocesium ( 137 Cs) (Bulko et al. 2014). Contamination of soils, pastures, and forests with the post-Chernobyl 137 Cs varied between the regions of Europe, and this fact highly impacted on regional appearance of 137 Cs in food, feed, mushrooms, grazing cattle, and wildlife and health risk of 137 Cs and other nuclides to human consumers (Barret et al. 1999;Battiston et al. 1989;De Cort et al. 1998;Smith et al. 1993;Strandberg and Knudsen 1994;Zarubina 2014). Some but minor (about 10 %) residual radioactivity from 137 Cs in the soils and wild-grown mushrooms still comes from the radioactive fallout which had taken place in the 1950s and 1960s because of the nuclear weapon tests in the atmosphere (Haselwandter et al. 1988;Steinhauser et al. 2013;Taira et al. 2011).
The nuclear power station in Fukushima Dai-ichi collapsed between 11th and 14th of March 2011 after a mega tsunami episode in the northeastern part of the Honshu Island-Tohoku region in Fukushima prefecture, Japan (Yasunari et al. 2011). In result of the Fukushima accident, a largescale diffusion of radioactivity took place. The radioactivity plume was largely dispersed in the ocean and in small portion on land there (Teramage et al. 2014). At the local scale, mushrooms in the prefecture of Fukushima have been identified as the most relevant source of radiocesium intake among vegetables, especially after the first year of the accident (Merz et al. 2015). Foraging of mushrooms bypass, wittingly or unwittingly, the governmental food measuring campaigns which leads to higher intake of radioactive cesium than when consumers bought their products in commercial shops (Hayano et al. 2013;Normile 2013).
The airborne 137 Cs that is deposited on land is efficiently taken up and sequestered in fruiting bodies by many wildgrown mushrooms (Macromycetes), which differ in their species-specific capacity to sequester stable Cs as well as many other metallic, non-metallic, and metalloid elements in the flesh (Bakken and Olsen 1990;Barret et al. 1999;Battiston et al. 1989;Brzostowski et al. 2011;Byrne et al. 1979;Eckl et al. 1986;Drewnowska and Falandysz 2015;Falandysz et al. 1994Falandysz et al. , 2003aFalandysz et al. , b, 2007aGucia et al. 2012;Kirchner and Daillant 1998;Kojta et al. 2012;Vinichuk et al. 2011;Zhang et al. 2010). These chemical elements, depending on their physical and chemical forms, can further be available from soil solution and soil bedrock to fungal mycelia, and sometimes, they can be sequestered in fungal flesh (fruiting bodies) more or less in a dose-effect-related manner. Hence, an elevated content of many metallic elements, metalloids, and Se (nonmetal) can be found in fruiting bodies of exposed populations, while the effectiveness of uptake and sequestration is a function of many variables including biological features related to species of mushroom, mycorrhiza, and geochemical/environmental factors (Falandysz and Borovička 2013).
Edible wild-grown mushrooms are popular organic food and are even items of international trade, and Boletus spp. are especially popular in Europe (King Bolete, Bay Bolete, Pine Wood) and especially in Yunnan and several other provinces in China (Falandysz et al. 2011;Wang et al. 2014). This paper reports and compares data on the residual activity from 137 Cs as well as natural radionuclide from 40 K accumulated in certain Boletus mushrooms collected in Poland, Sweden, and Belarus and in Yunnan of China. The contamination of King Bolete (Boletus edulis) from Yunnan (a land of mushrooms) is reported internationally for the first time. A major source of the residual 137 Cs for Poland without doubt is the Chernobyl accident (Mietelski et al. 2010), while for Yunnan, the likely sources include radioactive fallout from nuclear weapon tests in the 1950s and 1960s and possibly also because of the Chernobyl and Fukushima accidents.

Materials and methods
The Mushrooms from Belarus were collected from two spatially distant locations: Staroje Janczyna-location in central part, administrative circuit of Minsk, Borysowski Region, and Wasilewiczy in Chojniki area of the Gomel Region. Samples of B. edulis were also collected from the town of Umeå and its outskirts in northern region of Sweden and from Davingiie and Yimen in the Prefecture of Yuxi in the Yunnan Province of China. Fresh fruiting bodies' samples (separately caps and stipes) were sliced using a plastic knife, dried at 65°C to constant weight, and further pulverized using ceramic mortar and kept in brand new sealed polyethylene bags that were packed into larger bags and kept under dry and clean condition in a laboratory room until analysis. Before determination of activity concentration of radionuclides, the individual mushroom samples were pooled (separately caps and stipes, from 6 to 34 individuals per pool) to obtain one large (many specimens) integrated sample representing each place and year (Table 1).
Activity concentrations of 137 Cs and 40 K were determined using gamma spectrometer with coaxial HPGe detector with a relative efficiency of 18 % and a resolution of 1.9 keV at 1.332 meV (with associated electronics). The detector was coupled with an 8192-channel computer analyzer and GENIE 2000 software (Zalewska and Staniewski 2011). The equipment was calibrated using a multi-isotope standard, and the method was fully validated. The laboratory involved was subjected for routine checks to ensure the high standards of analytical quality and analytical control as well as took part in the intercomparison exercises organized by IAEA-MEL Monaco (IAEA-414, Irish and North Sea Fish) (Zalewska and Staniewski 2011) to verify the reliability and accuracy of the method. All numerical data gained were recalculated for dehydrated fungal material (at 105°C) and exact date of the sample collection.

Results
Data on radioactivity (expressed in Bq kg −1 dry matter) of 137 Cs and 40 K in caps and stipes of the Boletus mushrooms are summarized according to species, place of origin, size of sample, and year of collection ( Table 1) (Table 1).
The nuclide, 40 K, is a normal constituent of total K which is an important nutrient and the most abundant element in the fruiting bodies of mushrooms with a symbiotic or saprophyte life cycle. In B. edulis from Yimen in Yunnan, the activity of 40 K was similar to that noted for the samples from Poland and Sweden (Table 1). Significantly lower values, less than 120 Bq kg −1 dm in caps and less than 140 Bq kg −1 dm in stipes, were found in mushrooms collected in 2014 in the Dayingjie region of Yunnan (the same was observed for 40 K). This may be an indication of the deficiency of this important mineral nutrient in soils in Dayingjie, and this is worthy of further investigation.
Distribution of 40 K between the two morphological parts of the fruiting bodies for B. badius (caps and stipes) was nearly equal for most of the sites. The exception to this pattern was for samples from Wasilewiczy (Table 1). For B. edulis,   (Table 1). In contrast, the activity concentrations of 137 Cs in B. edulis from the Yuxi region of Yunnan were very low, i.e., from 5.2±1.7 to 10±1 Bq kg −1 dm for caps and 4.7±1.3 to 5.5±1.0 Bq kg −1 dm for stipes (Table 1).
High activity of 137 Cs were found also in other Boletus species: B. luridus collected from the forest with sandy soil bedrock at the Baltic Sea coastal place of the Sobieszewo Island near the city of Gdańsk, B. pinophilus from Puszcza Piska, and in B. reticulatus from Trójmiejski Landscape Park near the city of Gdynia (Table 1). The highest values were found in B. luridus; in 2000, the concentrations reached 3500±36 Bq kg −1 dm in caps and 1000±17 Bq kg −1 dm in stipes, while in B. pinophilus and in B. reticulatus, they were comparable and at the concentration of 1000 Bq kg −1 dm in caps and 400 Bq kg −1 dm in stipes. High levels of contamination with 137 Cs were found in B. reticulatus from the outsktits of Wasilewiczy in the Chojniki Distict in the Gomel region of Belarus (Fig. 1). The activity found in this area was 6600 ± 109 Bq kg −1 dm in caps and 3500 ± 30 Bq kg −1 dm in stipes, lower than the values observed in B. badius collected at the same place and time, indicating possible differences in the 137 Cs sequester capacity between these two species (Table 1).

Discussion
B. badius is well known to be susceptible to contamination with radiocesium (Malinowska et al. 2006). The samples from the post-Chernobyl polluted region of Gomel in Wasilewiczy, Belarus, collected in 2010 contained high amounts of 137 Cs, i.e., around 21,000 Bq kg −1 dry matter (dm) in caps and around 15,000 Bq kg −1 dm in stipes (Table 1). The activity of 137 Cs in B. badius from Poland varied depending on the sampling locations, and this could be roughly related to the regional differences in deposition of 137 Cs on and in soils because of the Chernobyl nuclear accident (Grodzinskaya and Haselwandter 2003;Haselwandter et al. 1988;Mietelski et al. 2010). The highest values of 5100±45 Bq kg −1 dm in caps and 4600±48 Bq kg −1 dm in stipes were found in samples from the Bory Tucholskie site collected in the year 2000. A slightly lower activity of 137 Cs, 4800±61 Bq kg −1 dm in caps and 2180±190 Bq kg −1 dm in stipes, was found in B. badius collected in 1995-1996 from the complex forests of the Wdzydze Landscape Park which is very close to Bory Tucholskie (Malinowska et al. 2006;Falandysz et al. 2003a, b). The mushroom B. badius from two other large forest complexes of the Puszcza Notecka within the outskirts of Porażyn (Fig. 1) that was sampled in 2008 showed much lower levels of contamination when compared to the corresponding values  Table 1) for mushrooms sampled elsewhere in Poland in the 1990s by other researchers with 970±8 Bq kg −1 dm in the caps and 45± 2 Bq kg −1 dm in the stipes (Table 1) (Malinowska et al. 2006).
The global radioactive fallout from nuclear weapon tests in the 1950s and 1960s and fallout from the Chernobyl accident has to be taken into account as a source of 137 Cs accumulated in B. edulis growing in Europe (García et al. 2015;Malinowska et al. 2006;Mietelski et al. 2010). The 137 Cs activity concentrations in B. badius and. B. reticulatus from Belarus, B. badius from Poland, and B. edulis from the Sudety Mountains in Poland are consistent with reported 137 Cs general picture and Bhot spot^deposition for regions of Belarus and Poland due to the Chernobyl accident (De Cort et al. 1998;Mietelski et al. 2010).
There is no information available to indicate that the most recent radioactivity release from the Fukushima accident affected Yunnan. Lack of gamma-ray radiation from 134 Cs in mushrooms sampled in Yunnan directly after the Fukushima accident in 2011 up to 2014 in this study (Table 1) and two other reports indicated that the contribution of Fukushima to the total radiocesium deposited there should be considered as negligible (Falandysz et al. 2015;Wang et al. 2015). On the other hand, earlier (pre-Fukishima accident period) data on the occurrence of 137 Cs in wild-grown mushrooms from Japan and Taiwan (in Asia) showed negligible contamination and thus can indirectly reflects depositions of small amounts of airborne 137 Cs after the Chernobyl accident and previous nuclear weapon tests in the atmosphere ( 134 Cs because of short life time, t ½ =2 years, decayed until Fukushima accident) (Muramatsu et al. 1991;Tsukada et al. 1998;Wang et al. 1998). Previous data on 134 Cs and 137 Cs in Boletus spp. from Yunnan and other regions of China are lacking. The activity concentrations from 137 Cs for samples from Yimen and Dayingjie (Yuxi Prefecture) were of the same order of magnitude, and this may indicate similarities in radioactive fallout there.
Low activity concentrations of 137 Cs determined in fruiting bodies of B. edulis from Yunnan presented in this study as well as in fruiting bodies of pan-tropical mushroom Macrocybe gigantea (median value for dehydrated caps was 4.5 Bq kg −1 and 5.4 Bq kg −1 for stipes) and sclerotia of fungus Wolfiporia extensa (range from <1.4 to 7.2±1.1 Bq kg −1 dm) (Falandysz et al. 2015;Wang et al. 2015) definitely imply that radioactive contamination, which could have resulted from both the recent (Fukushima) and earlier (Chernobyl and/or nuclear weapon tests) sources, is negligible in this region.
The relative abundance of 137 Cs in mushroom as determined in this study for Boletus mushrooms from Europe can be attributed to three factors: species-specific uptake, requirement of this (stable Cs) element by the mushroom, and lastly by forest soil contamination with 137 Cs at the sampling sites. Of secondary importance is the soil depth where the mushroom developed its mycelia, which is species-specific (Byrne 1998;Falandysz et al. 2014a;Stijve and Poretti 1990). The bulk of radioactive fallout as well as other airborne elemental contaminants are deposited and adsorbed on the top organic layer of forest soils. Some mushrooms with shallow mycelia can accumulate them readily and in considerable concentrations (Falandysz et al. 2014b;Mietelski et al. 2010;Stijve and Poretti 1990), and they subsequently infiltrate deeper into the soil layers (and the mycelia therein). This is dependent on the element's concentration, while topography, humidity of climate, and soil structure can favor quicker vertical passage of the element under consideration into the deeper layers of the soil alongside with infiltrating rain, taking a portion of nuclides that were not readily adsorbed by litter and organic horizon of soil into deeper layers (Teramage et al. 2014).
The low concentrations of 137 Cs found in B. edulis from Yunnan are important for the inhabitants of the region. One reason is because the Boletus mushrooms are very popular organic foods, and numerous species are collected in Yunnan, which is well known for the mushrooms that can be found there Wiejak et al. 2014;Zhang et al. 2010). Another reason is that in Yunnan, Boletes mushrooms are traditionally fried with hot vegetable oil using a wok (Chinese pan) but without pre-boiling (blanching). Blanching is a common procedure when cooking or pickling Boletus mushrooms in many countries including Poland. It results in the leaching out of some of the mushrooms' water and water-soluble constituents (including 137 Cs) into the water phase, thereby reducing their content in the final mushroom dish (Barret et al. 1999).
The radioactive isotopes 134 Cs and 137 Cs can account for the Btotal^Cs (stable Cs) content when measured using an instrumental method that is unable to differentiate between 134/137 Cs and 133 Cs. This is particularly important because the content of 134/137 Cs in mushrooms is associated with As stated earlier, there is a dearth of data on 137 Cs in mushrooms from China's mainland (Marzano et al. 2001). No available information is found concerning the content of 134 Cs and 137 Cs, stable 133 Cs, and 40 K in B. edulis from Yunnan or other closely related species like B. pinophilus or B. reticulatus-all three are naturally rich in selenium and certain other chalcophile elements (Falandysz 2008(Falandysz , 2013Falandysz et al. 2001aFalandysz et al. , 2007dFalandysz et al. , 2011Frankowska et al. 2010;Costa-Silva et al. 2011). They and some other related species are naturally more abundant in stable 133 Cs than many other mushrooms (Falandysz et al. 2001b(Falandysz et al. , 2007dHoryna and Řanda 1988).
The content of potassium (K) is high in fruiting bodies of the mycorrhizal type mushrooms, e.g., at 29,000 ± 3000 mg kg −1 dm in caps of B. edulis, from 38,000±4000 to 55,000±2000 mg kg −1 dm in Cantharellus cibarius, and from 28,000±3000 to 50,000±14,000 mg kg −1 dm in caps and from 21,000 ± 4000 to 35,000 ± 4000 in stipes of Suillus grevillei (Chudzyński and Falandysz 2008;Falandysz and Drewnowska 2015;Frankowska et al. 2010). Nuclide 40 K is a long-living isotope and is a natural part of total K, which is an essential element and undergoes a homeostatic regulation in fruiting bodies by mushrooms (Falandysz and Borovička 2013;Stijve 1996). 40 K is a dominant portion of the natural gammaradioactivity contained in the flesh of the fruiting bodies of mushrooms (Karadeniz and Yaprak 2010). The activity concentration of 40 K had a little fluctuation and was a substantial portion of the total gamma-radioactivity contained in the flesh of the fruiting bodies of all the Boletus mushrooms foraged in Europe in this study, and in practice, almost a solely source in samples from Yunnan, where >100-fold exceeded activity concentration of 137 Cs.
In conclusion, the amount of 40 K found in the fruiting bodies of a particular species of Boletus mushrooms collected from spatially distant places was more or less species-specific and stable with respect to time. On the other hand, a spatial pattern of activity of 137 Cs in these mushrooms was mosaic-like, and this could be attributed to differences in the density of fallout and local soil conditions. For some areas of land located well away from the Chernobyl nuclear unit, the mosaic-like pattern of 137 Cs accumulated in mushrooms (Boletus mushrooms) can reflect possible local differences in the density of fallout and the type of nuclides available to fungi when compared to what can be deduced from the expected pattern associated with pollution by 137 Cs in European soils. To get a better knowledge on exposure rates and risk to consumers from 137 Cs and other radionuclides in wild-grown mushrooms and especially the exposure to individuals, villagers and other high-level consumers of mushrooms need to be highlighted at any locality (e.g., forest) where mushrooms are highly contaminated. Falandysz J (2013)