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The contribution of Pir protein family to yeast cell surface display

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Abstract

Proteins with internal repeats (Pir) in the Baker’s yeast are located on the cell wall and include four highly homologous members. Recently, Pir proteins have become increasingly used as anchor proteins in yeast cell surface display systems. These display systems are classified into three types: N-terminal fusion, C-terminal fusion, and inserted fusion. In addition to the GPI (glycosylphosphatidyl inositol) and the FL/FS anchor proteins, these three Pir-based systems significantly increase the choices for target proteins to be displayed. Furthermore, Pir proteins can also be used as a fusion partner for target proteins to be effectively secreted into culture medium. Here, we summarize the development and application of Pir proteins as anchor proteins.

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Acknowledgments

This work was supported by the National Key Basic Research Development Plan (973 Project) (no. 2013CB127506), by The National Natural Science Foundation (no.31360130), by Hi-tech Research Development Plan of Yunnan Province (approval nos. 2009YG-3B), by Yunnan University (grant no. 2005Z004B), and by Tobacco’s Hi-tech Plan of Yunnan Province tobacco company (grant no. 2012YN06).

Conflict of interest

The authors declare that they have no conflict of interest. Na Yang and Zefen Yu contributed equally to this paper.

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Authors and Affiliations

  1. Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming, Yunnan, 650091, People’s Republic of China

    Na Yang, Zefen Yu, Dongchen Jia, Zhidan Xie, Keqin Zhang & Min Qiao

  2. Tobacco Research Institute of Yunnan Province, Kunming, Yunnan, 650091, People’s Republic of China

    Zhenyuan Xia & Liping Lei

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  1. Na Yang
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  2. Zefen Yu
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  3. Dongchen Jia
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  4. Zhidan Xie
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  8. Min Qiao
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Correspondence to Min Qiao.

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Yang, N., Yu, Z., Jia, D. et al. The contribution of Pir protein family to yeast cell surface display. Appl Microbiol Biotechnol 98, 2897–2905 (2014). https://doi.org/10.1007/s00253-014-5538-5

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  • DOI: https://doi.org/10.1007/s00253-014-5538-5

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