Abstract
Osteoarthritis is a common joint disease characterized by damage to the joint cartilage that occurs throughout the entire joint tissue. This damage primarily manifests as pain in the affected area. In clinical practice, medication is commonly used to relieve pain, but the treatment’s effectiveness is poor and recurrent attacks are likely. Schisandrin B is the most abundant biphenylcyclohexene lignan found in the traditional Chinese medicine Schisandra chinensis, and it possesses various pharmacological effects. This study aims to investigate the protective effect of Schisandrin B on mitochondrial damage in osteoarthritis (C28I2 cells) under an inflammatory environment induced by LPS. Cell proliferation and activity, scratch tests, and LDH release tests are utilized to assess cell growth and migration ability. The immunofluorescence assay was used to detect the expression levels of proliferation and apoptosis proteins. The Western Blot assay was used to detect the expression levels of mitochondrial fusion and division proteins. The JC-1 assay was used to detect changes in mitochondrial membrane potential. The mitochondrial fluorescence probe assay was used to detect mitochondrial activity. Through research, it was found that Schisandrin B promotes the proliferation, growth, and migration of C28I2 cells, reduces apoptosis of C28I2 cells, balances mitochondrial fusion and division, stabilizes mitochondrial membrane potential, and promotes mitochondrial activity in an LPS induced inflammatory environment.
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Each author expresses his gratitude to their institutions for providing the opportunity to develop this manuscript. Sincere thanks go to the co-authors for their contribution. The authors sincerely thank the anonymous reviewers for the improvement in the quality of the manuscript.
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This study was supported by Natural Science Foundation of Ningbo (2022J049).
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FH and WJH conducted experiments; FH writed the manuscript; HMX designed the study and revised the manuscript. All authors have read and approved the final version of the manuscript.
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Hu, F., Hu, W. & Xu, H. Schisandrin B Alleviates LPS Induced Mitochondrial Damage in C28I2 Cells. J Membrane Biol 257, 107–114 (2024). https://doi.org/10.1007/s00232-023-00299-5
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DOI: https://doi.org/10.1007/s00232-023-00299-5