How does extraction of biologically active substances with supercritical carbon dioxide affect lignocellulosic biomass properties?

In the bio-based economy concept, any biomass should not be used directly for energy purposes without considering the possibility of using it for bioproducts with higher added value. Extractable phytochemicals found in lignocellulosic biomass of perennial industrial crops (PIC) are used in the pharmaceutical, cosmetics, chemical, food and feed industries. Therefore, these compounds should be obtained first, and only the so-called PIC extraction biomass should be used, for example, for the production of bioenergy. An efficient low-energy and environmentally friendly method of extracting phytochemicals from the plant biomass is supercritical carbon dioxide (scCO2) extraction. The aim of the study was to assess the thermophysical properties and chemical composition of 19 types of PIC biomass previously subjected to two types of scCO2 extraction compared to the biomass before extraction. The biomass after pure scCO2 and scCO2/H2O (1 wt%) extraction contained less ash (by 4.9 and 11.3%), carbon (by 3.1 and 7.8%), hydrogen (by 5.8 and 8.9%), nitrogen (by 4.5 and 6.8%), sulfur (by 3.8 and 3.8%), lignin (by 5.4 and 1.1%), hemicellulose (by 14.4%) and more chlorine (by 4.7 and 15.3%) compared to the biomass before extraction. In addition, the biomass after extraction with pure scCO2 contained more cellulose and was characterized by lower moisture content and higher heating value and lower heating value. However, the biomass after scCO2/H2O extraction contained more substances soluble in cold and hot water. Generally, the bark of four short-rotation coppice (SRC) species was characterized by a high content of nitrogen, sulfur, substances soluble in cold and hot water, other soluble substances and at the same time low cellulose content (26–28% d.m.). In turn, the wood of all SRC species was characterized by a high cellulose content (51–56% d.m.). However, all herbaceous crops and grasses contained more chlorine, hemicellulose and generally less lignin compared to the SRC biomass. To assess the practical suitability of the PIC biomass extraction for the production of bioenergy (solid, liquid or gaseous biofuels), further research is needed.


Introduction
Perennial industrial crops (PICs), which include short-rotation coppice (SRC), herbaceous crops and grasses are one of the lignocellulosic biomass sources (Christian et al. 2008;Stolarski et al. 2013aStolarski et al. , 2014Stolarski et al. , 2017Sabatti et al. 2014;Ceotto et al. 2015Ceotto et al. , 2016Monti et al. 2015;Matyka and Kuś 2016). It should be emphasized that currently the use of the PIC biomass is associated mainly with its use for energy purposes, in order to generate thermal and electrical energy or transport biofuels using various conversion technologies (Godin et al. 2013;Stolarski et al. 2013bStolarski et al. , 2015aJankowski et al. 2016;Scordia et al. 2016). However, the future direction of using the PIC lignocellulosic biomass is the production of various renewable bioproducts, as part of a bio-based economy. The bio-based economy consists of all possible production paths for food and non-food products as well as energy services from biomass and requires a balanced supply of various biomass types for the production of bioproducts with multidirectional use. In the bio-based economy concept, each biomass, including the PIC lignocellulosic biomass, should be used in a sustainable manner. This means that it should not be used directly for energy purposes without considering the possibility of its use for high-value bioproducts. It should be emphasized that extractable phytochemicals found in PIC lignocellulosic biomass are used in industry, for example, pharmaceutical, cosmetics, chemical, food and feed (Attard et al. 2018;Bonaterra et al. 2010;Conde et al. 2014;Devappa et al. 2015;McElroy et al. 2017;Noleto-Dias et al. 2018;Parajuli et al. 2015;Sergent et al. 2014;Vázquez et al. 2012). Therefore, PIC lignocellulosic biomass should be obtained first and only residues, the so-called post-extraction biomass, should be used, for instance, for bioenergy production. The efficient low-energy and environmentally friendly method of extracting phytochemicals from the plant biomass is a supercritical carbon dioxide (scCO 2 ) extraction (Rój 2014). In addition, the use of scCO 2 extraction removes significant amounts of lipids and resin acids that easily undergo autoxidation, which consequently improves the safety of biofuel, for example, less off-gassing of wood pellets (Attard et al. 2016(Attard et al. , 2018. However, in order to consider the energy use of post-extraction biomass, it is necessary to know about its parameters, which are important for the production of solid, liquid or gaseous biofuels. The literature lacks information on the thermophysical and chemical properties of the PIC post-extraction biomass, from which biologically active substances have been extracted. Therefore, the aim of this research was to assess the thermophysical properties and chemical composition of 19 types of PIC lignocellulosic biomass previously subjected to two types of scCO 2 extraction in comparison with the biomass not subjected to the extraction.

Acquisition and preparation of biomass
The PICs were grown in field experiments located in northeastern Poland, on land owned by the University of Warmia and Mazury in Olsztyn. The studies were carried out on 11 PIC genotypes, including four species of short-rotation coppice (SRC): Salix viminalis L., Ekotur variety; S. purpurea L.; Populus nigra × P. maximowiczii Henry cv.  and Robinia pseudoacacia L.; three species of herbaceous crops: Helianthus salicifolius A. Dietr; Silphium perfoliatum L.; and H. tuberosus L.; but also four species of grasses: Miscanthus × giganteus J.M. Greef & M. Deuter; M. sacchariflorus ((Maxim.) Hack.); M. sinensis ((Thunb.) Andersson); and Spartina pectinata Bosc ex Link (Table 1).
The PIC lignocellulosic biomass was obtained in the winter period in the third decade of February 2018, from 1-year shoots formed on 7-year-old stumps (which were the plant stems from the entire growing period of 2017). The biomass of all genotypes was obtained using the brushcutter. In the case of four SRC species, three types of biomass were prepared, including bark, wood and a mixture of bark and wood. For this purpose, whole SRC shoots were cut out, followed by the bark mechanical separation from the wood. In this way, bark (b) and wood (w) were obtained. However, whole stems obtained directly from the plantations were used to obtain a mixture of bark and wood (b + w). Thus, 12 types of the biomass derived from SRC (4 species × 3 biomass types 12) were obtained in total. In the case of three genotypes of herbaceous crops (semi-wood) and four genotypes of grass (straw), the biomass was the whole shoots. Therefore, 19 samples of PIC lignocellulosic biomass were used for the studies (Table 1).

Supercritical fluid extraction
Each biomass was dried in a dryer at 40°C for 7 consecutive days to achieve a moisture content below 10%, followed by grinding in a hammer mill using a 1-mm sieve. After grinding the biomass, representative samples (approx. 0.5 kg) were collected for laboratory analyses of the raw material (prior to the extraction). Each of the 19 biomass samples (approx. 11 kg) was divided into two parts and subjected to the extraction of bioactive substances with carbon dioxide in a supercritical state. The extraction was performed with pure carbon dioxide (scCO 2 ) as well as with the addition of water as a co-solvent [scCO 2 /H 2 O (1 wt%)]. The supercritical fluid extraction was performed on a pilot plant with two extractors of 40 dm 3 each, working under the pressure of up to 1000 bar and temperature up to 100°C. Each raw material (5 kg) was extracted with scCO 2 and scCO 2 /H 2 O under the same parameters, which were set as follows: temperature at 40°C and pressure at 330 bar. After the extraction, representative samples (approx. 0.5 kg) of post-extraction residues were collected for laboratory analyses. It should be noted here that the choice of species and obtained biomass, the date of sampling and methods for extraction of bioactive substances are a part of the research project (see Acknowledgements).

Laboratory analyses
The samples (approx. 100 g each) of initial PIC biomass (19 samples) and the postextraction biomass (38 samples) were isolated in the laboratory in accordance with standard PN-EN ISO 14780:2017-07. The biomass moisture content was then determined at 105°C by a drying-weight method (PN-EN ISO 18134-1:2015) using a laboratory dryer (FD BINDER series, Tuttlingen, Germany). After moisture determination, the dried biomass samples were ground in an analytical mill using a 1-mm sieve (Retsch SM 200,Haan,Germany). Subsequently, the biomass samples were stored in closed laboratory containers. The higher heating value (HHV) of biomass was determined in the IKA C2000 calorimeter (Taufen, Germany) based on the dynamic method. The lower heating value (LHV) (PN-EN ISO 18125:2017-07) was calculated on the basis of HHV, moisture and hydrogen content. The ash content in the biomass was determined in the ELTRA TGA-THERMOSTEP automatic analyzer (Neuss, Germany) using the PN-EN ISO 18122:2016-01 standard.
As a part of the study, the elemental composition of biomass, the content of carbon (C), hydrogen (H) and sulfur (S) were determined using the ELTRA CHS-500 automatic analyzer (Neuss, Germany) (PN-EN ISO 16948:2015-07 andPN-EN ISO 16994:2016-10). In addition, the total nitrogen (N) was determined in the biomass by the Kjeldahl method using the K-435 mineralization apparatus and the BUCHI B-324 distillery apparatus (Flawil, Switzerland). The chlorine content was determined according to the PN-ISO 587:2000 standard. After samples burning in a NABERTHERM muffle furnace (Lilienthal, Germany) at a temperature of 650°C in the presence of an Eschka's mixture, the sample of an aqueous extract was titrated with 0.025 mol/L AgNO 3 .
The content of soluble substances in cold water was determined by weight based on the differences in the mass of the samples before and after the extraction. Samples placed in F57 filtration bags (ANKOM Technology) were extracted with distilled water (20-25°C) for 48 h. Then, they were rinsed twice in the ANKOM A200 apparatus (NY, USA) and dried before weighing (105°C). Weighed samples were used for further analyses. Similarly, the content of soluble substances in hot water was determined. In this case, the samples were extracted for 3 h at 100°C and dried prior to weighing at 105°C. An analytical balance with accuracy to 0.1 mg was used to weigh the samples.
After determination of substances soluble in hot water, laboratory analyses were performed to determine the NDF (neutral detergent fiber) (PN-EN ISO 16472:2007), ADF (acid detergent fiber) and ADL (acid detergent lignin) fractions (PN-EN ISO 13906:2009) in the biomass using the Ankom A200 extraction system. On the basis of differences in the obtained results, the content of lignin, cellulose and hemicellulose in the tested samples was calculated. The calculation methods are shown below.
The content of compounds soluble in cold water (CW) (1): The content of compounds soluble in hot water (HW) (2): The content of neutral detergent fiber (NDF) (3): The content of acid detergent fiber (ADF) (4): The content of acid detergent lignin (ADL) (5): where W 1 is the bag tare weight (g), W 2 -sample weight (g), W CW -dried weight of bag and samples after extraction in cold water (g), W HW -dried weight of bag and samples after extraction in hot water (g), W NDF -dried weight of bag with fiber after NDF extraction (g), W ADF -dried weight of bag with fiber after ADF extraction (g), C-blank bag correction (running average of final oven-dried weight divided by original blank bag weight), W ADL -dried weight of bag with fiber after ADL extraction (g), W A -weight of residue after incineration of the filter bag and samples at 520°C (g).

Statistical analysis
Two-factor analysis of variance in a fixed model was used for statistical data analyses. The significance of differences between means was assessed by Tukey's HSD test with P < 0.05. Additionally, in order to generalize a large number of results, two separate one-way analyses of variance were also performed. The first of these analyses compared in general two types of extraction (after pure scCO 2 and after scCO 2 /H 2 O) and biomass before extraction, regardless of the plant species. In the second one-way analysis, the main PIC groups were compared regardless of the type of extraction. The obtained results were collected in the form of means and homogenous groups of the Tukey's HSD test with P < 0.05. A multi-dimensional principal component analysis (PCA) with varimax rotation was also applied. All analyses were performed using the STATISTICA 13.3 package (TIBCO Software Inc. 2017).

Fiber and water-soluble fractions
The content of soluble substances in cold and hot water, other soluble substances, hemicellulose, cellulose and lignin was significantly differentiated by both extraction type and biomass type and the interactions between these main factors ( Table 2). The content of substances soluble in cold water in biomass after scCO 2 /H 2 O extraction was significantly higher (10.7% d.m. on average) compared to the biomass before extraction and after pure scCO 2 extraction, by 19 and 32% on average (Table 3). It was found that among the tested types of biomass, the content of substances soluble in cold water was by far the highest in the SRC bark and ranged from 13.8 to 26.2% d.m., respectively, for R. pseudoacacia (b) after extraction with pure scCO 2 and  *Detailed numerical data for all types of before extraction biomass and post-extraction biomass are presented in supplementary material in Tables S1 to S15 a, b, c -the values in the lines marked with the same letter do not differ statistically (Tukey's HSD test with P < 0.05) P. nigra × P. maximowiczii (b) in the biomass before extraction (Fig. 1). Lower values of this characteristic were found in the whole SRC biomass (average range from 5.6 to 9.9% d.m.), and even lower in wood (average range from 4.4 to 6.9% d.m.). In turn, in herbaceous crops biomass, the content of substances soluble in cold water ranged from 5.4 to 7.6% d.m. on average, for S. perfoliatum and H. salicifolius, respectively. However, the lowest value of this trait (on average 2.3% d.m) was determined in the biomass of S. pectinata grass. The content of substances soluble in hot water was generally higher compared to the content of substances soluble in cold water. On the other hand, the content of other soluble substances was higher in the post-extraction biomass compared with the  (Table 3). In other studies, the most soluble substances in cold (12.70% d.m.) and hot (19.64% d.m.) water and in the ethanol-benzene mixture (10.01% d.m.) were determined in the bark of 1-year-old willow shoots (Stolarski et al. 2005). In the cited study, the 1-year-old debarked willow shoots contained less of these compounds compared to bark and non-debarked shoots from 1-year and 2-year-old willow. On the other hand, the least soluble substances in cold (1.42% d.m.) and hot (3.90% d.m.) water were found in the wood of 4-year non-debarked shoots. The hemicellulose content (21.5% d.m. on average) in the biomass before extraction was significantly higher by approx. 14.4% compared to the biomass after pure scCO 2 and scCO 2 /H 2 O extraction (Table 3). Among the tested types of the biomass, S. pectinata grass was characterized by the highest content of hemicellulose (36-39% d.m.) (Fig. 2). Other grass species also had a high hemicellulose content, although it was lower in the range of 6 to 10 pp compared to S. pectinata. In the herbaceous crops, the hemicellulose content ranged from 15.5 to 22.3% d.m. in H. tuberosus after pure scCO 2 extraction and H. salicifolius before extraction, respectively. However, among the SRC, the high content of this feature (on average over 20% d.m.) was determined in wood (w) and in the whole biomass (b + w) of R. pseudoacacia. The lowest hemicellulose content was found in the SRC bark, ranging on average from 9 to 13% d.m., for S. viminalis and R. pseudoacacia, respectively. In other studies, the content of this compound in the 3-year-old willow biomass was higher (26.9% d.m. on average) compared to the values obtained for the SRC biomass in the present study ). An equally high content of hemicellulose (26.2% d.m.) was also determined in older poplar wood (Przybysz et al. 2018). However, its content in M. × giganteus biomass (25.9% d.m.) was close to the average value obtained in the present research. The cellulose content (47.0% d.m. on average) of the biomass after pure scCO 2 extraction was significantly higher compared to the biomass before and after scCO 2 /H 2 O extraction by approx. 6 and 7%, respectively (Table 3). Among the tested types of biomass, the wood (w) of S. viminalis and S. purpurea had the highest cellulose content (on average approx. 56% d.m.). A particularly high content of this compound was found after extraction with pure scCO 2 , 57.8 and 58.4% d.m., respectively, for S. viminalis (w) and S. purpurea (w) (Fig. 3), whereas the cellulose content in the wood of other SRC species was lower from 2 to 7 pp depending on the type of extraction. In turn, the cellulose content of the whole SRC biomass (b + w) ranged from 42.7 to 52.5% d.m., respectively, for P. nigra × P. maximowiczii after scCO 2 /H 2 O extraction and R. pseudoacacia after pure scCO 2 extraction. Among the herbaceous crops and grasses, the highest cellulose content was also determined in the biomass of H. tuberosus (53.6% d.m.) and M. × giganteus (52.8% d.m.) after extraction with pure scCO 2 . Among all tested types of biomass, the SRC bark was characterized by the lowest cellulose content (below 30% d.m.). Another research reports that the cellulose content in willow bark was lower than in wood and in the whole biomass (Stolarski et al. 2005). In the cited study, the effect of the age of the willow shoots on cellulose content was demonstrated and the lowest content of this compound was also found in all biomass of 1-year shoots (41.5% d.m.), and in 2-and 4-year shoots, this value was higher by about 44.8% d.m. It was also shown that the cellulose content can be significantly differentiated by willow species, variety and clone  ) in the biomass before extraction was significantly higher by 1.1 and 5.4% compared to the biomass after scCO 2 /H 2 O and pure scCO 2 extraction, respectively (Table 3). Among the tested types of biomass, R. pseudoacacia bark (b) was characterized by the highest content of lignin (26.6-23.8% d.m.) (Fig. 4). The bark of the remaining SRC species contained less lignin in the range of 2 to even 13 pp, and the lowest value of this trait (11.0% d.m.) was found in S. purpurea (b) after scCO 2 /H 2 O extraction. In contrast, the lignin content in the whole SRC biomass ranged from 17.3 to 20.9% d.m. for R. pseudoacacia biomass after pure scCO 2 extraction and S. purpurea biomass before extraction, respectively. In the group of herbaceous crops, the lignin content was lower and ranged from 11.5 to 17.3% d.m. for S. perfoliatum biomass before extraction and H. tuberosus biomass after both types of extraction, respectively. The biomass of the grasses had an even lower content of lignin, and the lowest value (8.15% d.m.) was determined in M. sinensis biomass after extraction with pure scCO 2 . According to the literature data, the lignin content in the willow bark of S. viminalis (37% d.m.) was almost two times higher than in wood (approx. 20% d.m.) and the whole biomass (approx. 22% d.m.) (Stolarski et al. 2005). An even higher lignin content was found in 3-year-old willow shoots, on average about 25% d.m. . In turn, the lignin content in the wood of poplar 'Hybrid 275' cultivar was 18.0% d.m., whereas in M. × giganteus it was lower only by 0.2 pp compared with poplar (Przybysz et al. 2018). It should also be added that in the present studies, hemicellulose loss in biomass after both supercritical extractions was greater than that of cellulose and lignin. The hemicellulose loss was due to its much greater susceptibility to degradation under the influence of scCO 2 and water, compared to other fiber fractions, which was also found in other studies (Haghighi Mood et al. 2013;Morais et al. 2015). Cellulose is a more durable polymer than hemicellulose; therefore, a slight loss in biomass was observed in most of the tested species, but only in the scCO 2 /water extraction variant. Under conditions of high pressure, carbon dioxide with water forms carbonic acid, which catalyzes the hydrolysis reaction of polysaccharides. However, hydrolysis results in a high efficiency at temperatures above 100°C (Morais et al. 2015). Lignin is an aromatic polymer and has hydrophobic properties. Lignocellulosic biomass is delignified during extraction using organic solvents and scCO 2 at temperatures above 150°C (Neata et al. 2015;Pasquini et al. 2005;Schrems et al. 2012). Therefore, in the present studies, a higher degree of lignin removal was observed in the case of pure scCO 2 extraction than scCO 2 /water. The degree of delignification and hydrolysis of cellulose and hemicellulose was much lower than in the works cited above, because the extraction studied here was carried out at a lower temperature and pressure. In the present research, biomass delignification was not the purpose of supercritical extraction. It should also be emphasized that the observed C and H loss was associated with the extraction of organic compounds with a higher C and H content than cellulose and hemicellulose. These compounds include such classes as fats, fatty acids, resin acids, waxes, hydrocarbons and polyphenols. Their isolation from lignocellulosic biomass was the main goal of the supercritical extraction process; therefore, a slight decrease in C and H content in post-extraction biomass was observed. On the other hand, it should be emphasized that in order to learn the exact mechanisms of cellulose, hemicellulose, lignin and element removal by supercritical extraction, additional, more detailed studies are needed.

Elemental composition
The elemental composition of biomass, including the content of carbon, hydrogen, sulfur and chlorine was significantly differentiated by both the type of extraction and the type of biomass and the interaction between these main factors ( Table 2). The content of carbon in biomass before extraction (approx. 55.7% d.m.) was significantly higher in comparison with the biomass after extraction with pure scCO 2 and scCO 2 /H 2 O by on average 3.1 and 7.8%, respectively (Table 3). Among the studied biomass, the wood of P. nigra ×P. maximowiczii was characterized by the highest content of carbon (average 56.5% d.m.) (Fig. 5). In general, the wood (w) of all SRC species was characterized by a higher carbon content as compared to its total biomass content (b + w) and bark (b). In turn, the content of carbon in herbaceous crops and grasses biomass was at a level similar to the SRC bark. According to other studies, among 26 PIC genotypes tested, the carbon content was also higher in the SRC biomass as compared to herbaceous crops and grasses (Stolarski et al. 2018). However, the marked contents of this element were lower and ranged from 47.8 to 52.8% d.m., respectively for S. perfoliatum and P. balsamifera UWM 2. Moreover, in the studies on 165 willow clones, the carbon content was on average at the level of 50-51% d.m. (Krzyżaniak et al. 2015;Stolarski et al. 2015b).
Similar to the content of carbon, the content of hydrogen in the biomass before extraction (on average 6.1% d.m.) was significantly higher compared with the biomass after extraction with pure scCO 2 and scCO 2 /H 2 O, by on average 5.8 and 8.9%, respectively (Table 3). Among the types of biomass studied, the highest hydrogen content (6.2% d.m.) was analyzed for R. pseudoacacia, whereas the lowest (5.4% d.m.) for S. perfoliatum (Fig. 6). Generally, the SRC biomass was characterized by a higher content of hydrogen as compared to herbaceous crops and grasses. Similar relationships were found in other studies in which the highest hydrogen content was found in the biomass of P. balsamifera UWM 2 (on average 6.2% d.m.), and the lowest in the biomass of S. perfoliatum (5.6% d.m.) (Stolarski et al. 2018). Based on the literature data, a high hydrogen content was determined in willow biomass (7.1% d.m.) (Cuiping et al. 2004).
The content of nitrogen in the biomass before extraction (average 0.88% d.m.) was also significantly higher by 4.5 and 6.8% in comparison with the biomass after extraction with pure scCO 2 and scCO 2 /H 2 O, respectively (Table 3). Among the tested types of biomass, the highest nitrogen content was found in the bark of R. pseudoacacia  (Fig. 7). Significantly, lower nitrogen content in the range from 0.4 to 0.5% d.m. was found in the SRC wood. As for herbaceous crops and grasses biomass, the content of nitrogen ranged from 0.43 to 0.69% d.m. for S. pecinata and M. sinensis, respectively. Stolarski et al. (2018) reported that among the 26 PIC species tested, the highest nitrogen content (approx. 1.5% d.m.) was determined in the biomass of R. pseudoacacia. However, in the majority of tested biomass, the average content of this element was below 1% d.m., whereas the lowest was on average 0.35% d.m. (S. hermaphrodita). The content of sulfur was higher by on average 3.8% in the biomass before extraction than in the two types of post-extraction biomasses ( Table 3). The higher sulfur content was clearly visible in the bark of all SRC species (range from 0.074 to 0.102% d.m.) compared to other types of biomass (Fig. 8). The grasses were characterized by lower sulfur content in the range of 17 to even 115% than in the SRC bark. Values similar to that in grasses were analyzed in the whole SRC biomass (b + w). In the case of the herbaceous crops biomass, the content of this element was on average below 0.04% d.m. However, by far the lowest value was in a pure SRC wood, on average from 0.023 to 0.038% d.m. in P. nigra x P. maximowiczii (w) and S. viminalis (w), respectively. Other studies also present significantly lower sulfur content in the SRC wood compared to bark (Stolarski et al. 2005). In contrast, in comparative studies of 26 PIC genotypes, by far the highest content of this element was found in the biomass of A. donax, on average 0.135% d.m., and the lowest in S. hermaphrodita, on average 0.031% d.m. (Stolarski et al., 2018). However, the analysis conducted on several hundreds of different willow and poplar clone biomasses revealed that the sulfur content was similar to that obtained in the present research and was approx. 0.05% d.m. (Krzyżaniak et al. 2015;Stolarski et al. 2015b;Monedero et al. 2016). Moreover, the sulfur content in the biomass in the present research and in the cited literature was very low compared to solid fossil fuels, as, for instance, the sulfur content in hard coal is in the range from 0.45 to even over 1% d.m. (Stolarski et al. 2013c;Wang et al. 2016). The chlorine content was the lowest in the biomass before extraction, on average 0.085% d.m. (Table 3). However, in the biomass after pure scCO 2 and scCO 2 /H 2 O extraction, it was higher by 4.7 and 15.3%, respectively. By far, the highest chlorine content (from 0.241 to 0.294% d.m.) was found in the biomass of H. salicifolius and for all three species of Miscanthus (Fig. 9). In another grass species, S. pectinata, the chlorine content was also high, on average approx. 0.110% d.m. However, significantly lower content of this element was found in S. perfoliatum from the group of herbaceous crops but also in all types of the SRC biomass.

Thermophysical properties of biomass
All studied thermophysical properties of biomass (moisture content, HHV, LHV and ash content) were influenced by both the type of extraction and the type of biomass and the interaction between these main factors ( Table 2). The biomass of all 19 analyzed samples after pure scCO 2 extraction was characterized by significantly lower moisture content (average 3.7%) compared to the biomass before extraction (average 6.1%) and that after scCO 2 /H 2 O extraction (average 7.6%) (Fig. 10, Table 3). In the case of extraction with pure carbon dioxide, the lowest moisture content was analyzed for the On the other hand, the moisture content of the biomass before and after scCO 2 /H 2 O extraction was in the range of 4.5-8.8% and 4.3-9.6%, respectively. Certainly, it should be added here that the very low moisture content of the analyzed biomass before extraction was due to the earlier thermal drying of the biomass as was required for the extraction. However, after the extraction with pure scCO 2 , further reduction in moisture content in the range of 2-3 percentage points (pp) was observed. On the other hand, in the case of the second type of extraction (scCO 2 /H 2 O), with water addition to carbon dioxide, the final moisture after extraction was also at the low level. This means that in the supercritical fluid extraction significant amounts of water from each type of biomass were discharged. Therefore, it can be concluded that after both types of extraction (scCO 2 and scCO 2 /H 2 O), the biomass moisture will be below 10%, which means that it can be successfully used for further processes or can be stored without losing its properties or values. This is very important because the moisture of PIC biomass harvested directly from the plantation is definitely higher and depends on the plant species, the type of biomass, weather conditions during harvesting but also the date of harvesting (Stolarski et al. , 2018. It was shown that among the SRC, particularly high moisture during harvesting may be observed for the biomass of poplar (53-62%), slightly lower for the biomass of willow (47-59%) and the lowest for the biomass of black locust (40-48%) (Krzyżaniak et al. 2015;Stolarski et al. 2013aStolarski et al. , 2018Nielsen et al. 2015;Gasol et al. 2010;Sabatii et al. 2014). On the other hand, the moisture content of the biomass from the herbaceous crops and grasses decreased as the harvest time was delayed, and at the end of March, it was at the lowest level, even below 20% (Stolarski et al. , 2018. The extraction with carbon dioxide in a supercritical state was used for dewatering of green wood before drying in an oven for less wood distortion (Dawson and Pearson 2017). The ash content in the biomass before extraction was the highest, on average 2.65% of dry matter (d.m.). The biomass extraction with pure scCO 2 and scCO 2 /H 2 O reduced the average ash content by 4.9 and 11.3%, respectively (Table 3). However, not all biomass types were characterized by the reduction in ash content as a result of the extraction. Among the analyzed types of biomass, the highest content of ash was observed for the bark of R. pseudoacacia, on average 5.0% d.m. (Fig. 11). It should be noted that in the case of the SRC biomass, the content of ash in the bark (b) was significantly higher compared to its content in wood (w) and in the mixture of bark and wood (b + w), even up to 5-8 times and 2-4 times higher, respectively than in wood (w) and bark + wood (b + w). The ash content in herbaceous crops biomass ranged from 2.8 to 4.3% d.m. in the biomass of H. tuberosus and S. perfoliatum, respectively. On the other hand, the value of this feature in the biomass of grasses was in the average range from 2.5 to 4.1% d.m. in M. × giganteus and M. sinensis, respectively. It should be emphasized, however, that the lowest ash content (in the range from 0.6 to 0.8% d.m.) among all biomass species was found in SRC wood, respectively for S. purpurea and P. nigra × P. maximowiczii. In addition, the ash content in the whole SRC biomass (b + w) was significantly lower (1.1-1.7% d.m.) compared to the ash content in the biomass of herbaceous crops and grasses. Similar relations between the ash content in the SRC biomass of herbaceous crops and grasses were found in other studies (Stolarski et al. , 2018. The biomass after extraction with pure scCO 2 was characterized by higher HHV and LHV values in comparison with the biomass before extraction and CO 2 /H 2 O extraction (Table 3). However, it should be noted that in the case of HHV, the differences were very small but were statistically significant, whereas LHV of the biomass after extraction with scCO 2 was significantly higher compared to the biomass before extraction and extraction with the addition of water to carbon dioxide (CO 2 /H 2 O) by 3.6 and 5.1%, respectively. The greater variation within LHV in comparison with HHV resulted mainly indirectly from the variation in the moisture content of raw materials, due to the fact that LHV is a function of moisture content, HHV and the content of hydrogen. When comparing the types of biomass in terms of HHV, higher values of this characteristic were found for the SRC, especially when compared to herbaceous crops as well as grasses (Fig. 12). However, in the case of LHV, the highest values of this feature were found for all 19 types of biomass after extraction with pure scCO 2 (Fig. 13). The LHV value for this type of biomass ranged from 16.34 to 17.84 MJ kg −1 for S. perfoliatum and P. nigra x P. maximowiczii (w), respectively. In addition, it should be emphasized that LHV of all 19 types of biomass, both after extraction with pure scCO 2 as well as scCO 2 /H 2 O (range from 15.53 to 17.19 MJ kg −1 ) and the biomass before extraction (range from 15.68 to 17.19 MJ kg −1 ), was higher compared to the biomass harvested directly from the plantation. This was mainly due to the low moisture content as shown in the present results. In other studies, it was shown that the LHV of the PIC biomass obtained directly from the plantations was significantly differentiated both by the species and by the harvest date (Stolarski et al. 2018). With the high moisture content of the biomass harvested from the plantation in winter, the LHV was Bold values indicate significance even 2-3 times lower compared to that found in post-extraction biomass. In the cited studies, the effect of the harvest date on LHV of herbaceous crops and grasses biomass was definitely higher (no such relationship was found for SRC). In the biomass harvested at the end of March, due to its natural drying, the LHV for H. tuberosus and M. sacchariflorus was high and amounted to 15.2 and 15.9 MJ kg −1 , respectively. High values of LHV biomass of herbaceous crops and grasses obtained in spring were also studied by other authors (Kalembasa and Malinowska 2008;Komorowicz et al. 2009;.

PCA analysis and impact assessment of PIC group on the biomass properties
In order to clearly illustrate the test results obtained within each extraction group, fifteen selected biomass features of the studied PIC species were subjected to multidimensional PCA analysis. In each case, this analysis separated three principal  (17), the lower the value of HHV, LHV, C and H (Fig. 14). However, all points lying in the center of the biplot are assigned to types of biomass with average values of the PC1 and PC2 component features. The last third component (PC3) is the content of chlorine, hemicellulose and the inversely correlated lignin content. In general terms, the PC3 component described hemicellulose and lignin content. This component explained less than 18% of variance (Table 4). Similar relationships were observed in both extraction groups: pure scCO 2 and scCO 2 /H 2 O. The exception was only moisture content, found in PC3 after scCO 2 /H 2 O extraction (Table 4, Figs. 15, 16). The present research shows that the chemical composition and thermophysical properties of the PIC biomass obtained in 1-year harvest cycles were very diverse. The quality of the biomass was determined in particular by the PIC species and the type of biomass: bark, wood, wood with bark, semi-wood and straw (Table 5). It was generally shown that bark biomass of all four SRC species had high content of nitrogen, sulfur, substances soluble in cold and hot water, other soluble substances and at the same time a low cellulose content. In turn, the wood of all SRC species was distinguished by a high cellulose content, whereas semi-woody biomass from the herbaceous crops group was characterized by lower HHV, LHV and carbon values. In addition, all herbaceous crops and grasses contained more chlorine, hemicellulose and generally less lignin compared to the SRC biomass.

Conclusion
The PIC biomass quality was determined by species and biomass type (bark, wood, wood with bark, semi-wood, straw) and by the type of extraction (pure scCO 2 and scCO 2 /H 2 O). It was shown that after both types of extraction (scCO 2 and scCO 2 /H 2 O), the biomass moisture content was below 10%, which means that it can be successfully used for further processes or can be stored without losing its properties or value. It was also found that the biomass after extraction with pure scCO 2 and scCO 2 /H 2 O contained less ash, carbon, hydrogen, nitrogen, sulfur, lignin, hemicellulose and more chlorine compared to the biomass before extraction. Moreover, the biomass after extraction with pure scCO 2 contained more cellulose and had lower moisture content and higher HHV and LHV values. However, the biomass after scCO 2 /H 2 O extraction contained more substances soluble in cold and hot water. The present results provide valuable and important information on changes in thermophysical properties and chemical composition of the PIC lignocellulosic biomass. Moreover, the post-extraction (pure scCO 2 and scCO 2 /H 2 O) residues, which are a byproduct, may be further used in a biorefinery or simply energy. Therefore, the present results can be used by other researchers and people involved in the production and use of PIC lignocellulosic biomass in the bioeconomy. Depending on the market demand for a given type of biomass with a specific composition, it is potentially possible to plan the production of the selected PIC species, followed by the entire logistics chain of biomass processing. However, in order to fully assess the usefulness of the PIC extraction biomass for the production of solid, liquid or gaseous biofuels, further research is needed. It should also be added and strongly emphasized that the biomass is not extracted in order to modify its properties but in order to obtain bioactive substances and other phytochemicals for bioproduct manufacturing. Acquisition of the largest possible amount of bioactive substances from a specified amount of biomass and in relation to, for instance, the surface unit from which the plant was obtained is also a key issue. Therefore, the issues of assessing the efficiency of supercritical extraction with pure scCO 2 and scCO 2 /H 2 O and the potential yield of the extract that can be obtained from the PIC cultivation area of 1 ha as well as the use of post-extraction biomass will be the subject of future work.