Abstract
Astrocyte-specific glutamate transporter subtype 1 (GLT-1) plays an important role in influencing glutamate excitatory toxicity and preventing the death of excitatory toxic neurons. Although the mammalian target of rapamycin (mTOR)/protein kinase B(Akt)/nuclear factor kappa B signaling cascade is involved in the upregulation of astrocytic GLT-1 in oxygen–glucose deprivation (OGD), it is unclear whether the mTOR/Akt pathway is involved in astrocytic GLT-1 upregulation in OGD and reoxygenation (OGD/R). In this study, we found that the treatment of cultured astrocytes with rapamycin and triciribine led to the decreased astrocytes’ protrusions, smaller nuclei, and an increased apoptotic rate. The inhibitors of mTOR complex 1 significantly increased the expression levels of phosphorylated Akt-Ser473 (p-Akt), phosphorylated Akt-Thr308(p-Akt), and GLT-1, while Akt-specific inhibitors blocked GLT-1 expression, suggesting that the mTOR/Akt pathway is involved in GLT-1 upregulation. We further demonstrated that astrocytes under OGD/R adapted to environmental changes through the mTOR/Akt pathway, mainly by altering cell morphology and apoptosis and upregulating the expression levels of p-Akt and GLT-1. Our results suggested that astrocytes may adapt to short-term ischemic–reperfusion injury by regulating cell morphology, apoptosis and GLT-1 upregulation.
Similar content being viewed by others
References
Abousaab A, Uzcategui NL, Elsir B, Lang F (2016) Up-regulation of the excitatory amino acid transporters EAAT1 and EAAT2 by mammalian target of rapamycin. Cell Physiol Biochem 39:2492–2500
Aizawa H, Sun W, Sugiyama K, Itou Y, Aida T, Cui WP, Toyoda S, Terai H, Yanagisawa M, Tanaka K (2020) Glial glutamate transporter GLT-1 determines susceptibility to spreading depression in the mouse cerebral cortex. Glia 68:2631–2642
Amin AG, Jeong SW, Gillick JL, Sursal T, Murali R, Gandhi CD (2021) Targeting the mTOR pathway using novel ATP-competitive inhibitors, Torin1, Torin2 and XL388, in the treatment of glioblastoma. Int J Oncol 59:83
Chen YF, Zhou XP (2020) Research progress of mTOR inhibitors. Eur J Med Chem 208:112820
Cipriani R, Chara JC, Rodríguez-Antigüedad A, Matute C (2015) FTY720 attenuates excitotoxicity and neuroinflammation. J Neuroinflammation 12:86
Gabryel B, Liber S (2018) Metformin limits apoptosis in primary rat cortical astrocytes subjected to oxygen and glucose deprivation. Folia Neuropathol 56:328–336
Gao Z, Zhang Z, Bian Q, Li Y, Ma D, Liu Z, Zhang S (2021) Mild hypothermia protects rat cortical neurons against oxygen-glucose deprivation/reoxygenation injury via the PI3K/Akt pathway. NeuroReport 32:312–320
Han JH, Park HS, Lee DH, Jo JH, Heo KS, Myung CS (2021) Regulation of autophagy by controlling Erk1/2 and mTOR for platelet-derived growth factor-BB-mediated vascular smooth muscle cell phenotype shift. Life Sci 267:118978
Hubbard JA, Szu JI, Yonan JM, Binder DK (2016) Regulation ofastrocyte glutamate transporter-1 (GLT1) and aquaporin-4 (AQP4) expression in a model of epilepsy. Exp Neurol 283:85–96
Jeong KH, Cho KO, Lee MY, Kim SY, Kim WJ (2021) Vascular endothelial growth factor receptor-3 regulates astroglial glutamate transporter-1 expression via mTOR activation in reactive astrocytes following pilocarpine-induced status epilepticus. Glia 69:296–309
Ji YF, Xu SM, Zhu J, Wang XX, Shen Y (2011) Insulin increases glutamate transporter GLT1 in cultured astrocytes. BiochemBiophys Res Commun 405:691–696
Ji YF, Zhou L, Xie YJ, Xu SM, Zhu J, Teng P, Shao CY, Wang Y, Luo JH, Shen Y (2013) Upregulation of glutamate transporter GLT-1 by mTOR-Akt-NF-кB cascade in astrocytic oxygen-glucose deprivation. Glia 61:1959–1975
Jia M, Njapo SA, Rastogi V, Hedna VS (2015) Taming glutamate excitotoxicity: strategic pathway modulation for neuroprotection. CNS Drugs 29:153–162
Karki P, Lee E, Aschner M (2013) Manganese neurotoxicity: a focus on glutamate transporters. Ann Occup Environ Med 25:4
Karki P, Smith K, Johnson J Jr, Aschner M, Lee E (2015) Role of transcription factor yin yang 1 in manganese-induced reduction of astrocytic glutamate transporters: putative mechanism for manganese-induced neurotoxicity. Neurochem Int 88:53–59
Karki P, Webb A, Smith K, Lee K, Son DS, Aschner M, Lee E (2014) Mechanism of raloxifene-induced upregulation of glutamate transporters in rat primary astrocytes. Glia 62:1270–1283
Krzyzanowska W, Pomierny B, Budziszewska B, Filip M, Pera J (2016) N-Acetylcysteine and ceftriaxone as preconditioning strategies in focal brain ischemia: influence on glutamate transporters expression. Neurotox Res 29(4):539–550
Kumar A, Rai S, Rathi E, Agarwal P, Kini SG (2021) Pharmacophore-guided fragment-based design of novel mammalian target of rapamycin inhibitors: extra precision docking, fingerprint-based 2D and atom-based 3D-QSAR modelling. Jbiomol Struct Dyn 39:1155–1173
Li LB, Toan SV, Zelenaia O, Watson DJ, Wolfe JH, Rothstein JD, Robinson MB (2006) Regulation of astrocytic glutamate transporter expression by Akt: evidence for a selective transcriptional effect on the GLT-1/EAAT2 subtype. J Neurochem 97:759–771
Liu X, Tian FF, Wang SQ, Wang F, Xiong LZ (2018) Astrocyte autophagy flux protects neurons against oxygen-glucose deprivation and ischemic/reperfusion injury. Rejuvenation Res 21:405–415
Mahmoud S, Gharagozloo M, Simard C, Gris D (2019) Astrocytes maintain glutamate homeostasis in the CNS by controlling the balance between glutamate uptake and release. Cells 8:184
Manning BD, Toker A (2017) AKT/PKB signaling: navigating the network. Cell 169:381–405
Martinez-Lozada Z, Robinson MB (2020) Reciprocal communication between astrocytes and endothelial cells is required for astrocytic glutamate transporter 1 (GLT-1) expression. Neurochem Int 139:104787
Men YP, Yelick JL, Jin SJ, Tian Y, Chiang MSR, Higashimori H, Brown E, Jarvis R, Yang YJ (2019) Exosome reporter mice reveal the involvement of exosomes in mediating neuron to astroglia communication in the CNS. Nat Commun 10:4136
Morel L, Regan M, Higashimori H, Ng SK, Esau C, Vidensky S, Rothstein J, Yang YJ (2013) Neuronal exosomal miRNA-dependent translational regulation of astroglial glutamate transporter GLT1. J Biol Chem 288:7105–7116
Munir M, Correale DM, Robinson MB (2000) Substrate-induced up-regulation of Na(+)-dependent glutamate transport activity. Neurochem Int 37:147–162
Pajarillo E, Rizor A, Lee J, Aschner M, Lee E (2019) The role of astrocytic glutamate transporters GLT-1 and GLAST in neurological disorders: potential targets for neurotherapeutics. Neuropharmacology 161:107559
Peterson AR, Binder DK (2019) Regulation of synaptosomal GLT-1 and GLAST during epileptogenesis. Neuroscience 411:185–201
Rao VL, Dogan A, Todd KG, Bowen KK, Kim BB, Rothstein JD, Dempsey RJ (2001) Antisense knockdown of the glial glutamate transporter GLT-1, but not the neuronal glutamate transporter EAAC1, exacerbates transient focal cerebral ischemia-induced neuronal damage in rat brain. J Neurosci 21(6):1876–1883
Romeo R, Glotzbach K, Scheller A, Faissner A (2021) Deletion of LRP1 from astrocytes modifies neuronal network activity in an in vitro model of the tripartite synapse. Front Cell Neurosci 14:567253
Schneider M, Dillinger AE, Ohlmann A, Iozzo RV, Fuchshofer R (2021) Decorin-an antagonist of TGF-β in astrocytes of the optic nerve. Int J Mol Sci 22:7660
Sun L, Li M, Ma X, Feng HY, Song JL, Lv C, He YJ (2017) Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent manner. J Neuroinflammation 14:231
Takano T, Wallace JT, Baldwin KT, Purkey AM, Uezu A, Courtland JL, Soderblom EJ, Shimogori T, Maness PF, Eroglu C, Soderling SH (2020) Chemico-genetic discovery of astrocytic control of inhibition in vivo. Nature 588:296–302
Tan XY, Luo MZ, Liu AP (2018) Clathrin-mediated endocytosis regulates fMLP-mediated neutrophil polarization. Heliyon 4:e00819
Wu CW, Storey KB (2021) mTOR signaling in metabolic stress adaptation. Biomolecules 11:681
Zha H, Fan YD, Yang L, Yin M, Miao W, He J, Wang Y (2021) Autophagy protects against cerebral ischemic reperfusion injury by inhibiting neuroinflammation. Am J Transl Res 13:4726–4737
Zhang WD, Li N, Du ZR, Zhang M, Chen S, Chen WF (2021) IGF-1 receptor is involved in the regulatory effects of icariin and icaritin in astrocytes under basal conditions and after an inflammatory challenge. Eur J Pharmacol 906:174269
Zhao CC, Jiang MY, Zhang LY, Hu YY, Hu ZJ, Zhang MY, Qi J, Su AC, Lou N, Xian XH, Zhang JG, Li WB, Zhang M (2019) Peroxisome proliferator-activated receptor gamma participates in the acquisition of brain ischemic tolerance induced by ischemic preconditioning via glial glutamate transporter 1 in vivo and in vitro. J Neurochem 151:608–625
Zhao YJ, Guo WY, Gu XL, Chang C, Wu J (2020) Repression of deoxynivalenol-triggered cytotoxicity and apoptosis by mannan/β-glucans from yeast cell wall: Involvement of autophagy and PI3K-AKT-mTOR signaling pathway. Int J Biol Macromol 164:1413–1421
Zhou JH, Tao K, Guo K, Wu L, Zhang ZG, Feng DY, Gao GD, Qin HZ (2020) Suppression of NDRG2 alleviates brain injury after intracerebral hemorrhage through mitigating astrocyte-drived glutamate neurotoxicity via NF-κB/GLT1 signaling. Brain Res 1729:146600
Acknowledgements
We are grateful for the helpful comments from members of the Organization Stem Cell Research Institute. This work was supported by grants from the National Natural Science Foundation of China (No. 81870966 to J.YF.). All of the authors declare that they do not have any conflicts of interest.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by Sreedharan Sajikumar.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Li, M., Yu, J., Deng, H. et al. Upregulation of glutamate transporter 1 by mTOR/Akt pathway in astrocyte culture during oxygen–glucose deprivation and reoxygenation. Exp Brain Res 241, 201–209 (2023). https://doi.org/10.1007/s00221-022-06514-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00221-022-06514-4