Abstract
Previous studies have indicated that interleukin-1β (IL-1β) is involved not only in immune modulation, but also in the modulation of pain in both the peripheral and central nervous systems. The current study investigated the expression of IL-1β in the brain of rats with spared nerve injury (SNI), using immunohistochemical technique. The results showed that immunoreactive-like IL-1β protein was significantly elevated in the Red nucleus (RN) 2 weeks after SNI. To further study the function of IL-1β in RN, different doses of IL-1β neutralizing antibody (10, 1.0 and 0.1 ng) were microinjected into the RN contralateral to the nerve injury side of neuropathic rats. The results indicated that the higher doses of anti-IL-1β antibody (10 and 1.0 ng) significantly attenuated the mechanical allodynia of neuropathic rats. However, administration of 0.1 ng anti-IL-1β antibody did not show anti-allodynia effect. These results suggest that IL-1β of RN is involved in the development of neuropathic pain in SNI rats.
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Abbreviations
- IL-1β:
-
Interleukin-1 beta
- SNI:
-
Spared nerve injury
- RN:
-
Red nucleus
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The Authors wish to thank Prof. Jingshi Tang, Prof. Shudong Qiu, Yuanyuan Jing and Dr. Wiaam Badn for their technical assistance and help with preparation of the manuscript.
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Wang, Z., Wang, J., Li, X. et al. Interleukin-1β of Red nucleus involved in the development of allodynia in spared nerve injury rats. Exp Brain Res 188, 379–384 (2008). https://doi.org/10.1007/s00221-008-1365-1
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DOI: https://doi.org/10.1007/s00221-008-1365-1