In vitro antioxidant and antibacterial activities with polyphenolic profiling of wild cherry, the European larch and sweet chestnut tree bark

This study is a comparative investigation of antioxidant and antibacterial properties of tree bark extracts of three common European species, Prunus avium L., Larix decidua Mill. and Castanea sativa Mill. The bioactive compounds present in the bark were recovered in 80% aqueous ethanol using ultrasound as the green extraction method. The antioxidant potential of the extracts was assessed with multiple biochemical assays: total phenol content (TPC) expressed in gallic acid equivalent (GAE), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) expressed in trolox equivalent (TE), and ferric reducing ability of plasma (FRAP) expressed in ascorbic acid equivalent (AAE). Sweet chestnut bark extract showed the highest antioxidant activity with TPC of 174.25 ± 16.95 mg GAE/g dry weight, DPPH (IC50) of 2.69 ± 0.03 μg/mL, ABTS of 739.65 ± 24.41 mg TE/g dry weight and FRAP of 207.49 ± 3.62 mg AAE/g dry weight. The antibacterial activity of the extracts was evaluated by disk diffusion test, minimal inhibitory concentration (MIC) assay and bacterial growth curves. Sweet chestnut bark extract gave IC50 values of 0.25 mg/mL and 1.00 mg/mL against E. coli and S. aureus, respectively. The polyphenolic profiling of the bark extracts was performed to identify the major compounds responsible for the bioactivities using high-performance liquid chromatography/tandem mass spectrometry (HPLC–MS/MS). The bark extracts were rich in natural antioxidants, thus holding tremendous potential for use as natural additives in food industry.


Introduction
In Europe, forests cover 215 million ha, i.e., one-third of the land area, and the forest-sector constitutes about 0.8% of its GDP giving a livelihood to over 3 million people [1]. Wood is the primary forest resource spanning over 150 million ha, which is used in furniture, construction, paper-making, and as a source of renewable energy. The processing of wood generates tons of bark that is mostly ineffectively dumped as landfill or incinerated. Bark protects the tree against external or functional damage, and prevents water loss. Recent studies have identified bark as a source of bioactive phenolic compounds for its valorization [2,3].
The polyphenols in plants are involved in their internal defense, regulation of growth, hormonal activity, pH and metabolism [4]. Furthermore, they exhibit numerous physiological properties such as antioxidant, antimicrobial, antiinflammatory, anti-carcinogenic, and therefore, have tremendous potential to be used in making dietary supplements, functional food additives, pharmaceutical products and cosmetics [5]. The extraction process is made challenging due to the different chemical structures of the compounds, their ability to degrade or hydrolyze and process selectivity towards the target analyte. The choice of extraction method and solvent depends on the plant matrix properties, physicochemical properties of analyte, analyte-solvent compatibility, process efficiency in terms of yield and purity, environmental impact, as well as the overall cost [3]. Ultrasound has proven to be very effective to enhance the yield and rate of mass transfer of extraction processes [6]. The principle of ultrasonic extraction is based on cavitation, i.e., the formation, growth and violent collapse of bubbles in the solvent medium giving rise to local hotspots. The resulting physical effects cause the fragmentation and erosion of the plant cells, increased solvent penetration (sonocapillary effect) and subsequent washing out of the cell contents (sonoporation) [7].
Limited literature is available on the extraction, characterization and biological effects of the bioactive compounds from tree bark. The bark extracts of Quercus robur, Alnus glutinosa, Larix decidua and Picea abies showed high free radical scavenging activity, elastase and collagenase inhibitory activities for use in the dermo-cosmetic industry [8]. Another study tested the antifungal activity of bark extracts of several European trees including Norway maple (Acer platanoides L.), which significantly inhibited the growth of Fomitopsis pinicola (brown rot fungi) and Heterobasidion parviporum (white rot fungi) [9]. In this study, three commercially relevant tree species viz., wild cherry (Prunus avium L.), the European larch (Larix decidua Mill.) and sweet chestnut (Castanea sativa Mill.), which are common in Hungarian forests were chosen. Earlier studies have shown that these species have exceptionally high antioxidant capacity in their bark as well as antimicrobial effects [10][11][12]. Moreover, in Europe, these trees are abundant and easily accessible (Fig. 1). The wild cherry is a fast growing and short-lived deciduous tree with edible sweet fruits and dense wood that is widely used in veneer production, cabinet-making and paneling [1]. Although the wild cherry fruits and other vegetal parts have been widely investigated [13,14], only a few studies are available on its bark [8]. The European larch is a large deciduous, coniferous tree, typically adapted to continental alpine climates [1]. Its wood is durable due to high tannin content and is used in carpentry, outdoor and naval construction, as well as for pulping. Few recent works have examined the European larch bark for its active constituents and bioactivity [15,16]. The sweet chestnut, a long-living deciduous variety adapted to warmtemperate climate, is a multi-purpose tree cultivated for its timber and nuts [1]. An earlier study has linked sweet chestnut bark with neuroprotective effects against oxidative stress [17].
The aim of the present work was to extract and identify bioactive compounds, especially polyphenols from these species using ultrasound followed by a comparative analysis of their bioactivities. The polyphenols were extracted in aqueous ethanol due to its low toxicity and ecological impact. The antioxidant properties of bark extracts were evaluated using Folin-Ciocâlteu total phenol content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, and the ferric reducing ability of plasma (FRAP); while the antibacterial activities against E. coli and S. aureus were determined using disk diffusion test (DDT), minimal inhibitory concentration (MIC) assay and bacterial growth curves. The bioactive compounds were characterized by high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)/tandem mass spectrometry (MS/MS) technique. The study provides a comprehensive investigation and comparative assessment of the polyphenolic constituents and their correlation with the bioactivities of the tree bark. It is expected to provide useful insights towards the development of natural additives for food.
Standard strains of Staphylococcus aureus (CCM 4223) and Escherichia coli (CCM 3954) were obtained from the Czech collection of microorganisms (Brno, Czech Republic). The strains were cultivated over night at 37 °C on 5% Columbia blood agar (Sigma-Aldrich, Germany).

Sampling of tree bark
Whole bark samples were collected from the tree trunks of Prunus avium L., Larix decidua Mill. and Castanea sativa Mill. originating from the forests of TAEG (Tanulmányi Erdőgazdaság) Forestry Company, Sopron (Hungary) during December 2019. The samples were dried in air at room temperature for 3 days in the dark. The dried samples were subsequently ground, sieved and stored in plastic bags at −20 °C. The meshed fraction in the range of 0.2-0.63 mm was used for extraction. The moisture content of the samples was found to be 6-10% using an infrared moisture analyzer (Sartorius MA35).

Extraction of bioactive compounds assisted by ultrasound
The extraction was done using a horn sonicator (Tesla 150 WS) consisting of a titanium horn (18 mm in diameter) operating at an ultrasonic frequency of 20 kHz with a maximum power output of 150 W. Bark specimens were extracted in 80% aqueous ethanol for 15 min at full amplitude, and a solid to solvent ratio of 1:100 (g/mL) with 34.9% ultrasonic horn energy efficiency, based on our earlier work [18]. The filtered extracts were stored in amber-colored glass bottles under refrigeration at −20 °C for antioxidant and liquid chromatographic analyses. The extract solutions were evaporated to obtain dry powder for antibacterial testing.

Determination of TPC
The TPC assay was run according to the procedure of Singleton and Rossi [19]. In a typical experiment, 500 μL of the extract and 2.5 mL of Folin-Ciocâlteu reagent were thoroughly mixed together, followed by the addition of 2 mL of Na 2 CO 3 solution (0.7 M) after 1 min. The reaction mixture was incubated in a water bath at 50 °C for 5 min.
The absorbance was measured at 760 nm against the blank solution on a UV-VIS spectrophotometer (Hitachi U-1500).
The mean values of three replicates were expressed in mg equivalents of gallic acid/g dry weight of specimen (mg GAE/g dw).

Determination of DPPH scavenging activity
The DPPH radical scavenging assay was performed using the method of Sharma and Bhat with some modifications [20]. The assay involved dilution of 10 μL of extract with 2090 μL of unbuffered methanol and the subsequent addition of 900 μL of DPPH. It was incubated at ambient temperature away from light for 30 min and the drop in absorbance was measured at 515 nm. The calibration curve was plotted by measuring absorbance at different dilutions of a standard methanolic solution of DPPH (2 × 10 -4 M). The mean values of three replicates were expressed as IC 50 (50% inhibition concentration) in μg extractives/mL.

Determination of ABTS scavenging activity
The ABTS radical scavenging assay was done according to the protocol of Stratil et al. [21]. A solution of ABTS radical (7 mM) and potassium persulfate (12.5 mM) was prepared to have an absorbance of 0.70 ± 0.02 at 734 nm. For the assay, 1960 μL of the radical solution was mixed well with 40 μL of the extract followed by incubation at ambient temperature for 10 min. The absorbance was measured at 734 nm in dim light. The calibration curve was plotted using serial dilutions of a standard trolox solution (1 mM). The mean values of three replicates were expressed in mg equivalents of trolox/g dry weight of specimen (mg TE/g dw).

Determination of FRAP
The FRAP was performed in accordance with the procedure of Benzie and Strain [22]. In a typical run, 50 μL of the extract was mixed with 1500 μL of FRAP reagent, allowing the reaction to occur in the dark at room temperature. After 5 min, the absorbance was measured at 593 nm against the blank solution, while standard solutions of ascorbic acid were used for the calibration curve. The mean values of three replicates were expressed in mg equivalents of ascorbic acid/g dry weight of specimen (mg AAE/g dw).

Estimation of extractive content
To estimate the extractive content (yield), aliquots of the extracts were dried in an oven at 40 °C. The residual solids were weighed on a digital scale (Sartorius MSA225P) and the yield was expressed in mg extractives/mL extract units. The results were taken into account for the determination of DPPH IC 50 values.

Sample preparation
The dried extracts of wild cherry, the European larch, and sweet chestnut were dissolved in 1% DMSO to give a final concentration of 1 mg/mL. The prepared extracts were evaluated for their antibacterial activity against Gram-positive bacteria, Staphylococcus aureus (CCM 4223) and Gramnegative bacteria, Escherichia coli (CCM 3954). For reproducibility, the disk diffusion test and the growth curve assay were performed in duplicate, while the minimal inhibitory concentration assay was performed in tetraplicate.

Disk diffusion test (DDT)
The antibacterial activity of the extracts was determined by DDT performed according to the EUCAST 2019 guidelines [23]. Inoculum (10 µL) of each standard strain was prepared from a suspension adjusted to 0.5 McFarland density using deionized water. Amoxicillin and Penicillin were used as positive controls for Gram-negative and Gram-positive bacterial strains, respectively. The inoculated plates were incubated at 37 °C for 12 h.

Minimal inhibitory concentration (MIC) assay
The MIC assay was performed according to EUCAST 2020 guidelines in 96-well microtiter plates [24]. Extracts were diluted in Mueller Hinton Broth (Sigma-Aldrich, Germany) to achieve a two-fold dilution from 1.000 mg/mL to 0.016 mg/mL. Standard strain of 0.5 McFarland density was diluted 100× using deionized water to give cell density of 1-2 × 10 6 CFU/mL. Cultures were incubated at 37 °C on plate shaker at 120 rpm. The absorbance at 620 nm was monitored at time zero, and then after 15 h of incubation. The results were expressed as IC 50 values (mg/mL), which is the concentration of extracts that caused a 50% inhibition of the tested bacteria.

Bacterial growth curves
Extract concentrations from 1.000 mg/mL to 0.031 mg/mL were obtained by the broth dilution method. The procedure for sample preparation and concentration range were same as for the MIC assay. The growth curve of bacteria in the presence of the extract was measured by a Bioscreen C MBR (Dynex, Czech Republic) in 100-well microtiter plates. The absorbance at 620 nm was monitored at time zero, and then at 30 min intervals for 24 h, while the culture was incubated at 37 °C.

The HPLC-PDA-ESI-MS/MS characterization of the extracts
Separation of bark extract constituents was achieved using a Shimadzu LC-20 type high-performance liquid chromatograph coupled with a Shimadzu SPD-M20A photodiode array detector (PDA) (Shimadzu Corporation, Kyoto, Japan) and an AB Sciex 3200 QTrap triple quadrupole/linear ion trap mass spectrometer (MS) (AB Sciex, Framingham, USA). The stationary phase used a Phenomenex Synergy Fusion-RP 80A, 250 mm × 4.6 mm, 4 µm column with a Phenomenex SecurityGuard ULTRA LC type guard column (Phenomenex Inc., Torrance, USA) at 40 °C. The injection volume was 8 µL. Gradient elution was run using A (H 2 O + 0.1% HCOOH) and B (CH 3 CN + 0.1% HCOOH) solvents with 1.2 mL/min flow-rate with the following schedule: 3% B (0-4 min), 6% B (10 min), 20% B (34 min), 57% B (73 min), 100% B (90-98 min), 3% B (99-106 min). The PDA signal (250-380 nm) was recorded to monitor the separation of peaks. A negative electrospray ionization mode was set for the MS detector by allowing 0.6 mL/min flow to enter the MS ion source using a split valve. Polyphenol structures were analyzed and identified with the information dependent analysis (IDA) function of the mass spectrometer using survey (Q1) scans between 150 and 1300 m/z and dependent (Q3) product ion scans between 80 and 1300 m/z. Ion source settings were as follows: spray voltage was −4500 V, source temperature was 500 °C; curtain gas, spray gas and drying gas (N 2 ) pressures were 40 psi, 30 psi, and 30 psi, respectively. Chromatographic data were evaluated using the Analyst 1.6.3 software. Mass spectra evaluation and compound identification was achieved using tandem mass spectral databases and data found in the literature.

Statistical analysis
All the antioxidant assays were done in triplicate and the results were expressed as mean ± standard deviations of the three values. The experimental data on the various bark species were compared by analysis of variance (ANOVA) on Statistica 11 (StatSoft Inc., Tulsa, USA) software applying the Tukey Post Hoc test at a 95% level of confidence.

Antioxidant activity of bark extracts
Plants produce a wide range of phenolic antioxidants including flavonoids, proanthocyanidins, cinnamic acids, benzoic acids, coumarins, stilbenes, lignans and lignins. The antioxidant activity of polyphenols is mainly influenced by their chemical structure, ability to form hydrogen bonds, the capability of metal ions for chelation and reduction, kinetic solvents effect, adduct formation, and reduction potential [4]. The TPC assay is based on the reaction of phenolic compounds in the extract with a colorimetric reagent due to the transfer of electrons between them in an alkaline medium. Table 1 shows the results of the various antioxidant assays for the three bark species. Among the investigated species, the highest TPC was obtained for the chestnut bark (174.25 ± 16.95 mg GAE/g dw), while the lowest TPC was obtained for the cherry bark (112.88 ± 17.27 mg GAE/g dw). Many studies have explored the sweet chestnut tree for its phenolic content, particularly the shells, burs and leaves [25,26]; but only a few can be found on the bark [27]. Interestingly, chestnut leaves and burs showed lower phenolic contents of 115.4 ± 1.8 and 93.3 ± 2.4 mg GAE/g dw, respectively, compared to the bark [28]; while cherry fruits showed TPC of 284.48 ± 3.07 GAE mg/100 g of fresh cherries [14]. One of the earlier studies evaluated the phenolic content of the bark extracts after separating the outer bark from the inner bark [10]; although for practical reasons and from a commercialization point of view, we chose to study the whole bark of the tree trunks. Other studies have evaluated the TPC values of bark of Quercus rubra (276.50 ± 3.23 mg GAE/g dw extract), Betula celtiberica (432.02 ± 3.00 mg GAE/g dw extract) [27], Larix laricina (34 ± 2 g GAE/100 g extract) [29], as well as Fagus sylvatica L. (65.22 ± 5.57 mg GAE/g dw) [30]. Reports on bark extraction using methods such as solid-liquid extraction with aqueous methanol [28], and subcritical water extraction [31] showed a lesser yield of phenolics.
The DPPH assay is commonly used to evaluate the scavenging potential of extract constituents against the DPPH free radicals. Although the assay does not reflect the overall in vivo scavenging potential, it gives rapid and reproducible results [32]. Very limited literature is available on the antioxidant properties of bark extracts, DPPH being the most widely employed method and commonly expressed in µg/ mL as IC 50 values or in mg equivalent of a standard/g dw. The phenolic compounds act as excellent oxygen radical scavengers due to their lower electron reduction potential; thus, a high phenolic content can be correlated to a high radical scavenging capacity [32]. Accordingly, as indicated by the IC 50 values in Table 1, the bark extracts of larch and chestnut showed high DPPH scavenging capacities of 2.54 ± 0.15 and 2.69 ± 0.03 µg/mL, respectively, that were not significantly different from each other. The IC 50 value of larch bark was found to be lower than that of its cones (13.73 ± 1.30 µg/mL) [33]. Similarly, the IC 50 value of chestnut bark was lower than that of its leaves (7.05 ± 0.66 µg/mL) [34]. On the other hand, the cherry bark exhibited the least IC 50 value of 4.31 ± 0.18 µg/mL, which was lower than the IC 50 value of cherry leaves (27.29 ± 0.77 µg/mL) and flowers (61.59 ± 0.71 µg/mL) [13]. These IC 50 values are comparable to DPPH scavenging activity of bark extracts of Juniperus oxycedrus (1.1 µg/mL) [35], and Fagus sylvatica L. (7.45 ± 0.07 µg/mL) [30]. A similar trend on DPPH activity was observed in a study on bark extracts of temperate trees including wild cherry and the European larch using magnetic stirring in ambient conditions and methanol as the solvent [8]. Another study used pressurized hot water as an extraction medium for larch industrial waste and found the bark had a higher IC 50 value (0.3 mg/mL) than the branches and the sapwood, but not the heartwood [36]. Notably, these values were considerably lower than those in this work, which may be primarily attributed to the extraction intensification using ultrasonic horn that enhanced the yield of polyphenols. The ABTS assay gives the ABTS radical scavenging capacity expressed in trolox equivalents, hence it is also known as trolox equivalent antioxidant capacity (TEAC). It has been widely used due to its simplicity for screening of hydrophilic and lipophilic compounds, as the radical is soluble in both water and organic solvents. However, like DPPH, ABTS does not occur naturally, inviting possible criticism that the assay is not directly relevant to real biological environments [30]. As evident from Table 1, the sweet chestnut bark had the highest ABTS scavenging activity of 739.65 ± 24.41 mg TE/g dw. This value was much higher than that of chestnut leaves (323.63 ± 16.00 mg TE/g dw) [34], as well as chestnut shells (156.59 mg AAE/g dw) [37]; thus indicating higher quenching capacity of the chestnut bark. In contrast, bark extracts of the European larch and wild cherry showed lower scavenging activities of 432.25 ± 45.23 and 424.90 ± 18.30 mg TE/g dw, respectively, not differing significantly from each other. It is interesting to note that while the DPPH scavenging activity of the European larch was similar to that of sweet chestnut, its ABTS scavenging activity was only a little over half that of the latter. This may possibly be explained by the diverse selectivity of methods to different compounds and various modes of action of the antioxidants [32]. It should be pointed out that both inner bark and outer bark of the tree trunk influence the antioxidant properties of the bark. A study found that the inner bark showed higher antioxidant capacity compared to the outer bark for most species [10]. The variations in genetic factors (cultivars) or geographic locations may also affect the metabolite profile [38]. The results obtained in this study were comparable to those of bark extracts of Fraxinus angustifolia (4.5 μM TE/mg extract) [39], and Goniothalamus velutinus (78.88 ± 0.56 mg TE/g extract) [40]. Nevertheless, the reported values for radical scavenging assays in the literature may vary due to differences in the experimental conditions, thus making the comparison difficult. Another drawback is that the antioxidant-free radical reaction may not reach completion within the designated time span, resulting in an underestimation of its actual value [41].
The FRAP assay is based on low-pH reduction of a colorless ferric complex to a blue-colored ferrous complex by antioxidants acting as electron-donors. It uses inexpensive reagents and is straightforward to perform, giving highly reproducible results [22]. A downside of the assay is that it not only measures antioxidants but also other compounds having a redox potential lesser than 0.77 V and thus are capable of reducing the ferric ion [30]. The FRAP antioxidant capacity in Table 1 is consistent with the other antioxidant assays, with sweet chestnut bark having the highest antioxidant capacity of 207.49 ± 3.62 mg AAE/g dw. The European larch bark showed FRAP value of 84.08 ± 3.15 mg AAE/g dw, which was over twice that of its green cones (40.39 ± 0.73 mg AAE/g dw) [33]. Wild cherry bark showed the least FRAP value of 72.26 ± 1.08 mg AAE/g dw. These results were significantly higher compared to the FRAP values of bark extracts of Fagus sylvatica L. (49.69 ± 3.44 mg AAE/g dw) [30], and Eucalyptus globulus (7.81 mmol AAE/100 g dry bark) [42]. Other studies reported the FRAP antioxidant capacity in trolox equivalent such as for Quercus robur (640.30 ± 22.03 mg TE/g dried extract) [27], and Goniothalamus velutinus (80.11 ± 1.52 mg TE/g dried extract) [40]. In all of the antioxidant assays, aqueous ethanol proved to be a solvent with a very high extraction efficiency for the phenolic antioxidants, which has also been pointed out in a number of previous studies [27,29]. Furthermore, the use of ultrasound to recover natural antioxidants proved to be an efficient method that enhanced the extraction yield with reduced solvent consumption and time; the mechanism of which is already established [6]. Thus, the bark species investigated here hold an immense potential for valorization due to their rich phenolic content and high antioxidant capacity, considering the growing interest for the possible utilization of natural bioactive compounds, especially in the food sector.

Antibacterial activity of bark extracts
The antibacterial properties of wild cherry, the European larch and sweet chestnut bark extracts were investigated using DDT, MIC assay and bacterial growth curves. Earlier studies have shown antimicrobial properties in extracts of larch bark [43][44][45]. The DDT is a qualitative method used for the detection of antimicrobial property [46]. In this study, the extracts exhibited a very weak inhibition against S. aureus and no inhibition against E. coli, as shown in Table 2.
The MIC assay results for bark extracts in the concentration range of 0.016-1.0 mg/mL showed that all extracts inhibited the growth of both standard strains after 15 h of incubation. Visible inhibition for S. aureus and E. coli was observed in the entire concentration range. As shown in Table 3, IC 50 values were obtained for all extracts (≤ 0.5  [15]. In contrast, cherry leaf extract gave MIC value of 50 mg/mL for the two strains [47], while chestnut leaf and shell extracts showed MIC values of 25 mg/mL and 50 mg/mL, respectively, for S. aureus [25]. Bacterial concentrations in the presence of bark extracts were recorded over 24 h to obtain the growth curves shown in Fig. 2. It was observed that the extracts had no antibacterial activity against E. coli (Fig. 2a, b, c) and supported their growth. In contrast, significant inhibitory effect of the extracts was observed on S. aureus (Fig. 2d, e, f). These results did not completely correlate with the MIC assay results, since IC 50 values were obtained for both S. aureus and E. coli. With wild cherry, significant inhibition was shown at 0.063 mg/mL and higher extract concentrations for S. aureus (Fig. 2d). With the European larch and sweet chestnut, the inhibition effects were observed at extract concentrations of 0.500 mg/mL (Fig. 2e) and 0.250 mg/ mL (Fig. 2f), respectively, or lower values. The fact that the extracts showed antibacterial activity against S. aureus and no activity against E. coli correlates with DDT results.
A noteworthy observation in this study is the higher efficacy of bark extracts against Gram-positive S. aureus than Gram-negative E. coli. This trend has also been observed in previous studies. For example, the larch bark extracts were reported to give MIC values of 1.2 mg/mL and 1.75 mg/mL against S. aureus [48,49]. In contrast, it showed no antibacterial activity against Gram-negative strains [43,48]. Similar results for MIC have also been found for wild cherry bark, as in this study. According to Abedini et al. (2020), the wild cherry bark extract was the most effective against Grampositive and Gram-negative bacterial strains at MIC values of 0.125 mg/mL and 0.250 mg/mL, respectively [48]. Arora and Mahajan (2018) investigated the antimicrobial activity of a wild Himalayan cherry bark extract against several bacterial strains and observed MIC values of 1.0 mg/mL against E. coli and 5.0 mg/mL against S. aureus [50]. Likewise, the promising antibacterial activity of sweet chestnut bark has been demonstrated [51].

Polyphenolic composition of bark extracts
Altogether 123 compounds were tentatively identified and described from bark tissues of wild cherry, the European larch and sweet chestnut. Figure 3 depicts the UV chromatograms while Table 4 lists the major compounds identified in the extracts. Identification was based on literature data and tandem mass spectral databases. Some of the compounds including (+)-catechin (13) (32), scopolin (20) as well as catechin isomers (13,27), which are all flavonoids and flavonoid glycosides. The derivatives of dihydrowogonin and apigenin were first evidenced in wild cherry bark by Geibel and Feucht (1991) [52]. The presence of dihydrowogonin (91, 92), scopoletin (31), taxifolin (37), genistein (86), aromadendrin (51), and naringenin (81) in wild cherry bark has already been evidenced in a previous study [48]. However, the authors of the study found high diversity in flavonoids (mainly flavanones and flavonols) in aglycone form and only lower levels of glycosylated kaempferol, taxifolin and naringenin derivatives. On the other hand, in this study, mostly the glycoside conjugates of polyphenolic compounds were found rather than their respective free aglycones. The ratio of glycoside conjugates and their respective free aglycones in bark extracts depends on many factors including age, type of sample collection (ratio of inner and outer bark), storage and drying, as well as on extraction solvent and method [53]. The significance of polyphenolic compounds in contributing to the antibacterial properties of wild cherry bark extracts was reported by Oyetayo and Bada (2017) [47]. Abedini et al. (2020) also emphasized the role of dihydrowogonin as a potentially strong antimicrobial compound.

Conclusion
In summary, we have carried out a comparative study on the in vitro antioxidant and antibacterial properties of bark extracts of wild cherry, the European larch and sweet chestnut trees. Among them, the sweet chestnut bark showed the highest antioxidant potential, which also correlated with its highest total phenol content. All the extracts demonstrated significant antibacterial effect against Gram-positive S. aureus compared to Gram-negative E. coli. Interestingly, sweet chestnut also seemed to be the most effective against the tested bacterial strains. The presence of various polyphenolic compounds in the extracts contributing to the bioactivities was confirmed by HPLC-MS/MS analysis. Another notable aspect of the work was the use of ultrasound as a green route and aqueous ethanol as the solvent medium for the extraction of bioactive compounds from the bark. Since bark extracts are naturally rich in phenolic antioxidants, they hold promise for potential utilization as natural additives