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Influence of sequential inoculation of Wickerhamomyces anomalus and Saccharomyces cerevisiae in the quality of red wines

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Abstract

The use of mixed inoculation of non-Saccharomyces yeasts and S. cerevisiae in wine fermentations is of increasing interest for quality enhancement and improved complexity of wines. The effect of sequential inoculation of Wickerhamomyces anomalus (formerly Hansenula anomala) with interesting enological properties in terms of secondary metabolite production and a commercial S. cerevisiae strain in fermentation of non-sterilized red musts from Mazuela variety has been examined. The wines elaborated by sequential inoculation presented higher levels of acetates and ethyl esters, compounds that supply a fruity note, higher levels of lineal alcohols, which are responsible for herbaceous notes and lower concentrations of organic acids, that contribute to increase the aromatic quality, than wines produced by a S. cerevisiae monoculture. Both types of wines were comparable in levels of volatile acidity, glycerol, lactic acid and succinic acid produced. Sensory analysis showed that red wines obtained by mixed fermentations were preferred by 71.5 % of the tasters and were particularly appreciated for its floral and/or fruity notes.

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References

  1. Dubourdieu D, Tominaga T, Mansneuf I, Peyrot des Gachons C, Murat ML (2006) The role of yeast in grape flavour development during fermentation: the example of Sauvignon Blanc. Am J Enol Vitic 57:81–88

    CAS  Google Scholar 

  2. Heard GM, Fleet GH (1985) Growth of natural yeast flora during the fermentation of inoculated wines. Appl Environ Microbiol 50:727–728

    CAS  Google Scholar 

  3. Salvadó Z, Arroyo-López FN, Guillamón JM, Salazar G, Querol A, Barrio E (2011) Temperature Adaptation Markedly Determines Evolution within the Genus Saccharomyces. Appl Environ Microbiol 77:2292–2302

    Article  Google Scholar 

  4. Gil JV, Mateo JJ, Jiménez M, Pastor A, Huerta T (1996) Aroma compounds in wine as influenced by apiculate yeasts. J Food Sci 61:1247–1249

    Article  CAS  Google Scholar 

  5. Bely M, Stoeckle P, Masneuf-Pomared I, Dubourdieu D (2008) Impact of mixed Torulaspora delbrueckii-Saccharomyces cerevisiae culture on high-sugar fermentation. Int J Food Microbiol 122:312–320

    Article  CAS  Google Scholar 

  6. Jolly NP, Augustyn OPH, Pretorius IS (2006) The role and use of non-Saccharomyces yeasts in wine production. S Afr J Enol Vitic 27:15–38

    CAS  Google Scholar 

  7. Ciani M, Comitini F, Mannazzu I, Domizio P (2010) Controlled mixed culture fermentation: a new perspective on the use of non-Saccharomyces yeasts in winemaking. FEMS Yeast Res 10:123–133

    Article  CAS  Google Scholar 

  8. Izquierdo Cañas PM, Palacios García AT, García Romero E (2011) Enhancement of flavour properties in wines using sequential inoculations of non-Saccharomyces (Hansenula and Torulaspora) and Saccharomyces yeast starter. Vitis 50:177–182

    Google Scholar 

  9. Egli CM, Edinger WD, Mitrakul CM, Henick-Kling T (1998) Dynamics of indigenous and inoculated yeast populations and their effect on the sensory character of Riesling and Chardonnay wines. J Appl Microbiol 85:779–789

    Article  CAS  Google Scholar 

  10. Henick-Kling T, Edinger W, Daniel P, Monk P (1998) Selective effects of sulphur dioxide and yeast starter addition on indigenous yeast populations and sensory characteristics of wine. J Appl Microbiol 84:865–876

    Article  CAS  Google Scholar 

  11. Fleet GH, Heard GM (1993) Yeasts growth during fermentation. In: Fleet GH (ed) Wine microbiology and biotechnology. Harwood Academic Publishers, Chur, pp 27–54

    Google Scholar 

  12. Fleet GH (2008) Wine yeasts for the future. FEMS Yeast Res 8:979–995

    Article  CAS  Google Scholar 

  13. Ferraro L, Fatichenti F, Ciani M (2000) Pilot scale vinification process using immobilized Candida stellata cells and Saccharomyces cerevisiae. Process Biochem 35:1125–1129

    Article  CAS  Google Scholar 

  14. Toro ME, Vázquez F (2002) Fermentation behaviour of controlled mixed and sequential cultures of Candida cantarelli and Saccharomyces cerevisiae wine yeast. World J Microbiol Biotechnol 18:347–354

    Article  CAS  Google Scholar 

  15. Rojas V, Gil JV, Pinaga F, Manzanares P (2003) Acetate ester formation in wine by mixed cultures in laboratory fermentations. Int J Food Microbiol 86:181–188

    Article  CAS  Google Scholar 

  16. Viana F, Gil JV, Genovés S, Vallés S, Manzanares P (2008) Rational selection of non-Saccharomyces wine yeasts for mixed starters based on ester formation and enological traits. Food Microbiol 25:778–785

    Article  CAS  Google Scholar 

  17. Mckay AM (1990) Degradation of polygalacturonic acid by Saccharomyces cerevisiae. Lett Microbiol 11:41–44

    Article  CAS  Google Scholar 

  18. Strauss MLA, Jolly NP, Lambrechts MG, Van Rensburg P (2001) Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeasts. J Appl Microbiol 91:182–190

    Article  CAS  Google Scholar 

  19. Charoenchai C, Fleet GH, Hensechke PA, Todd BEN (1997) Screening of non-Saccharomyces wine yeasts for the presence of extracellular hydrolytic enzymes. Aust J Grape Wine Res 3:2–8

    Article  CAS  Google Scholar 

  20. Fernández M, Ubeda JF, Briones AI (2000) Typing of non-Saccharomyces yeasts with enzymes activities. Int J Food Microbiol 59:29–36

    Article  Google Scholar 

  21. Kurtzman C, Fell JW, Boekhout T (2011) The yeasts a taxonomic study, 5th edn. Elsevier, Amsterdam

    Google Scholar 

  22. Izquierdo PM, Ubeda J, Briones A (1997) Study of the karyotype of wine yeasts isolated in the region of Valdepeñas in two consecutives vintages. Food Microbiol 14:221–225

    Article  Google Scholar 

  23. Legras JL, Karst F (2003) Optimisation of interdelta for Saccharomyces cerevisiae strain characterization. FEMS Microbiol Lett 221:249–255

    Article  CAS  Google Scholar 

  24. OIV Organización Internacional de la Viña y el Vino (2011) Compendium of international methods of wine and must analysis

  25. Ribereau-Gayon P, Stronestreet E (1965) Le dosage des anthoccyanes dans les vins rouges. Bull Soc Chim 9:2649–2652

    CAS  Google Scholar 

  26. Somers TC, Evans ME (1976) Spectral evaluation of young red wines: anthocyanin equilibria, total phenolics, free and molecular SO2, “Chemical age”. J Sci Food Agric 28:279–287

    Article  Google Scholar 

  27. Nagel CW, Glories Y (1991) Use a modified dimethylaminocinnamaldehyde reagent for analysis of flavanols. Am J Enol Vitic 42:364–366

    CAS  Google Scholar 

  28. Vivas N, Glories V, Lagune L, Sancier C, Augustin M (1994) Estimation de degreé de polymérisation des procyanidines du raisin et du vin par la méthode au p-dimethylaminocinnamaldéhyde. J Int Sci Vigne Vin 28:319–336

    CAS  Google Scholar 

  29. Smith PA (2005) Development of an assay to quantify tannins in grape and wine 419 samples. Aust Wine Res Inst Technol Rev 154:37–38

    Google Scholar 

  30. Ibarz M, Ferreira V, Hernandez-Orte P, Loscos N, Cacho J (2006) Optimization and evaluation of a procedure for the gas chromatographic-mass spectrometric analysis of the aromas generated by fast acid hydrolysis of flavors precursors extracted from grapes. J Chromatogr A 1116:217–229

    Article  Google Scholar 

  31. ISO Standard 4120, (1983) Sensory Analysis. Methodology for triangular test

  32. ISO Standard 11035 (1994) Sensory analysis. Identification and selection of descriptors for establishing a sensory profile

  33. Renouf V, Claisse O, Lonvaud-Funel A (2007) Inventory and monitoring of wine microbial consortia. Appl Microbiol Biotechnol 75:149–164

    Article  CAS  Google Scholar 

  34. Plata C, Millán C, Mauricio JC, Ortega JM (2003) Formation of ethyl acetate and isoamyl acetate by various species of wine yeasts. Food Microbiol 20:217–224

    Article  CAS  Google Scholar 

  35. Romano P, Suzzi G, Domizio P, Fatichenti F (1997) Secondary products formation as a tool for discriminating non-Saccharomyces wine strains. Antonie Van Leeuwenhoek 71:239–242

    Article  CAS  Google Scholar 

  36. Azzolini M, Tosi E, Vagnoli P, Krieger S, Zapparoli G (2010) Evaluation of technological effects of yeast–bacterial co-inoculation in red table wine production. Ital J Food Sci 3:257–263

    Google Scholar 

  37. Domizio P, Romani C, Lencioni L, Comitini F, Gobbi M, Mannazzu I, Ciani M (2011) Outlining a future for non-Saccharomyces yeasts: selection of putative spoilage wine strains to be used in association with Saccharomyces cerevisiae for grape juice fermentation. Int J Food Microbiol 147:170–180

    Article  CAS  Google Scholar 

  38. Lambrechts MG, Pretorius IS (2000) Yeast and its importance to wine aroma. A Review. S Afr J Enol Vitic 21:97–129

    CAS  Google Scholar 

  39. Ribereau-Gayon P (1978) Wine aroma. In: Charalambous G, Inglett GE (eds) Flavour of foods and beverages. Academic Press, NewYork, pp 362–371

    Google Scholar 

  40. Izquierdo Cañas PM, García E, Gómez S, Palop ML (2008) Changes in the aromatic composition of Tempranillo wines during spontaneous malolactic fermentation. J Food Compost Anal 21:724–730

    Article  Google Scholar 

  41. Etiévant X (1991) Wine. In: Maarse H (ed) Volatile compounds in food and beverages. Dekker, New York, pp 483–546

    Google Scholar 

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Acknowledgments

P. M. Izquierdo and M. Fernández-González are grateful to the INCRECYT program and the European Social Fund for financial support.

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This article does not contain any studies with human or animal subjects.

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Correspondence to Mónica Fernández-González.

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Izquierdo Cañas, P.M., García-Romero, E., Heras Manso, J.M. et al. Influence of sequential inoculation of Wickerhamomyces anomalus and Saccharomyces cerevisiae in the quality of red wines. Eur Food Res Technol 239, 279–286 (2014). https://doi.org/10.1007/s00217-014-2220-1

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  • DOI: https://doi.org/10.1007/s00217-014-2220-1

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