Abstract.
2-Aminobutylamino-4-ethylamino-6-isopropylamino-1,3,5-triazine (ABA–atrazine) has been synthesized and used as a coating hapten in an immunoassay with a monoclonal antibody against terbutryn. Coating was achieved by covalently linking ABA–atrazine to a glutaraldehyde polymer network directly bound to the polystyrene surface of a standard 96-well microtiter plate. The assay was carefully optimized. In particular, the coating hapten concentration had a strong effect on the ELISA sensitivity. By including a pre-incubation step a low test midpoint (IC50-value) of 0.130 µg L–1 was achieved. As far as we are aware this is the most sensitive ELISA for terbutryn yet reported. The coating-hapten-format presented is proposed as generally applicable, because the glutaraldehyde-modified microtiter plate surface enables stable immobilization of a broad variety of coating haptens.
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Holthues, H., Pfeifer-Fukumura, U., Hartmann, I. et al. An immunoassay for terbutryn using direct hapten linkage to a glutaraldehyde network on the polystyrene surface of standard microtiter plates. Fresenius J Anal Chem 371, 897–902 (2001). https://doi.org/10.1007/s002160101050
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DOI: https://doi.org/10.1007/s002160101050