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Metal-doped carbon dots as peroxidase mimic for hydrogen peroxide and glucose detection

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Abstract

Carbon dots (CDs) have several superior characteristics including sufficient carbon sources, easy preparation, no toxicity, and high catalytic efficiency as a new kind of nanozyme. Herein, Ce-doped carbon dots (Ce-CDs), Cr-doped carbon dots (Cr-CDs), Cu-doped carbon dots (Cu-CDs), Fe-doped carbon dots (Fe-CDs), Mn-doped carbon dots (Mn-CDs), and non-metal-doped carbon dots (0-CDs) were synthesized to explore the detection of hydrogen peroxide (H2O2) and glucose as peroxidase mimic. The prepared CDs could efficiently oxidize the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxTMB in the presence of H2O2. After adding glucose oxidase (GOD) to the CDs/TMB system, a colorimetric method for glucose detection was developed. The results show that Fe-CDs possess the highest catalytic activity. When using Fe-CDs as peroxidase mimetics, the detection limit of this assay for glucose was 0.029 mmol L−1. This successfully provides a sensitive and selective colorimetric method for hydrogen peroxide and glucose determination.

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Funding

We received financial support from the National Natural Science Foundation of China (21804075), Qingdao Postdoctoral Applied Research Project, Taishan Scholar Program of Shandong Province (ts201511027), and the Natural Science Foundation of Shandong (ZR2020BM063).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Qingdao, 266071, People’s Republic of China

    Xing Yuxin, Sun Laipeng, Liu Kang, Shi Haipeng, Wang Zonghua & Wang Wenjing

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  1. Xing Yuxin
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  2. Sun Laipeng
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  3. Liu Kang
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  4. Shi Haipeng
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  5. Wang Zonghua
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  6. Wang Wenjing
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Corresponding authors

Correspondence to Wang Zonghua or Wang Wenjing.

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All applicable international, national, and/or institutional guidelines for the collection and use of human blood and urine samples were followed. Blood and urine samples were collected from anonymized volunteers with their own requests from The Affiliated Hospital of Qingdao University (Qingdao, China) with the permission of local ethical committees.

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Supplementary file1 Supplementary data (Fig. S1-Fig. S9) to this article can be found in the File (Supporting Information). (DOC 1267 KB)

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Yuxin, X., Laipeng, S., Kang, L. et al. Metal-doped carbon dots as peroxidase mimic for hydrogen peroxide and glucose detection. Anal Bioanal Chem 414, 5857–5867 (2022). https://doi.org/10.1007/s00216-022-04149-6

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  • DOI: https://doi.org/10.1007/s00216-022-04149-6

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