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Visual upconversion nanoparticle-based immunochromatographic assay for the semi-quantitative detection of sibutramine

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Abstract

Immunochromatographic assay (ICA) has been used widely for the onsite monitoring of illegal additives due to its simplicity, speed, and low cost. However, a scanner is commonly required for ICA to achieve quantitative results. In this work, we developed a visual semi-quantitative ICA for sibutramine, a banned additive in diet foods, without the need for a scanner for measurement. Monoclonal antibodies specific for sibutramine were raised and conjugated with upconversion nanoparticles (UCNPs) as the luminescent tracer. ICA was developed by employing multiple test lines to achieve the semi-quantitative detection of sibutramine. Based on the optimal conditions, the cutoff levels (limit of quantitation, LOQ) of T1 line, T2 line, T3 line, and T4 line were 0.02 μg/mL, 0.15 μg/mL, 1.0 μg/mL, and 7.5 μg/mL, respectively, in buffer system. The ICA demonstrated a LOQ at 0.2 mg/kg for sibutramine in diet food samples. The assay (including pretreatment) can be finished within 30 min without the aid of other instruments, except a laser pen. No false positive or false negative results were observed. The results indicated that the proposed method was reliable, simple, and rapid for the screening of sibutramine abuse in diet food samples.

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Funding

This work was financially supported by the Key-Area Research and Development Program of Guangdong Province (2019B020211002), the Guangdong Special Support Program (2019TX05N052), and the Generic Technique Innovation Team Construction of Modern Agriculture of Guangdong Province (2019KJ130).

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, Guangdong, China

    Shi-Wei Zhang, Yuan-Ming Sun, Hong Wang & Zhen-Lin Xu

  2. National Nutrition Food Testing Center, Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, 518055, Guangdong, China

    Shi-Wei Zhang

  3. Department of Anaesthesiology, Shenzhen University General Hospital, Shenzhen, 518055, Guangdong, China

    Yan-Yan Sun

  4. Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California Davis, Davis, CA, 95616, USA

    Zhen-Feng Li

Authors
  1. Shi-Wei Zhang
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  2. Yan-Yan Sun
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  3. Yuan-Ming Sun
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  4. Hong Wang
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  5. Zhen-Feng Li
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  6. Zhen-Lin Xu
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Correspondence to Yan-Yan Sun or Zhen-Lin Xu.

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This article does not contain any studies with human subjects. All animal experiments that described in the present study were performed in adherence to South China Agricultural University animal experiment center guidelines and approved by Animal Ethics Committee.

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Zhang, SW., Sun, YY., Sun, YM. et al. Visual upconversion nanoparticle-based immunochromatographic assay for the semi-quantitative detection of sibutramine. Anal Bioanal Chem 412, 8135–8144 (2020). https://doi.org/10.1007/s00216-020-02944-7

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  • DOI: https://doi.org/10.1007/s00216-020-02944-7

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