Abstract
Senecio graciliflorus DC root extract was studied for secondary metabolite composition following the bioactivity-guided isolation technique. The ethyl acetate extract of Senecio graciliflorus root yielded nine chemical constituents: 3,4-di-tert-butyl toluene, stigmasterol, β-sitosterol, 2β-(angeloyloxy)furanoeremophilane, gallic acid, 2β-{[(Z)-2-hydroxymethylbut-2-enoyl]oxy}furanoeremophilane, 1-hydroxypentan-2-yl-4-methylbenzoate, sarcinic acid, and sitosterol 3-O-β-d-glucopyranoside. The structures of the chemical constituents were elucidated on the basis of spectral data analysis in the light of literature. All the compounds are being reported for the first time from this plant. The isolated constituents were screened for neuroprotective effects against corticosterone-induced impairment in neuroblastoma cell lines (SH-SY5S cells). The viability of SH-SY5S cells was determined using MTT assay. Among various isolated compounds, three natural products (sarcinic acid, gallic acid, and β-sitosterol) displayed robust neurotropic activity. The compounds increased neuronal cell survival in differentiated neuroblastoma cells (SH-SY5Y) from high-dose corticosterone (400 µM)–induced cell death. All the three constituents showed maximum AKT/ERK pathway activation at 20 µM concentration. The studies are aimed to explore small molecules for treating neurodegeneration underlying various neurological disorders to restore neuronal cell plasticity.
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01 June 2022
A Correction to this paper has been published: https://doi.org/10.1007/s00210-022-02257-9
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The research work was partly funded by CSIR and DST by providing fellowship to Showkat Ahmad Bhat and Loveleena Kaur.
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Khursheed Ahmad Bhat and Fayaz A. Malik conceived and designed the research work, supervised the experimental work, interpreted the experimental data, and participated in writing the manuscript. Salman Jameel and Loveleena Kaur did the experimental work and participated in writing the manuscript. Showkat Ahmad Bhat contributed to the isolation of pure natural products. All authors approved the final version of the article. The authors declare that all data were generated in-house and that no paper mill was used.
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Jameel, S., Kaur, L., Bhat, S.A. et al. Neuroprotective activity of natural products isolated from Senecio graciliflorus DC against corticosterone-induced impairment in SH-SY5Y cells. Naunyn-Schmiedeberg's Arch Pharmacol 394, 2389–2399 (2021). https://doi.org/10.1007/s00210-021-02136-9
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DOI: https://doi.org/10.1007/s00210-021-02136-9