Abstract
A facultative anaerobic nitrogen-fixing bacterium, designated SG131T, was isolated from paddy soil. Strain SG131T showed high 16S rRNA gene sequence similarities with type strains Propionivibrio limicola DSM 6832T (96.9%), Propionivibrio pelophilus asp 66T (96.0%) and Propionivibrio dicarboxylicus DSM 5885T (95.7%). The phylogenetic trees (based on 16S rRNA gene sequences and 120 conserved genes from genomes, respectively) indicated that strain SG131T clustered with members of the genus Propionivibrio. Growth of strain SG131T was observed at 25–40 °C, pH 5.5–10.5 and 0–0.5% (w/v) NaCl. The quinone was Q-7, and the main fatty acids were C16:1 ω6c and/or C16:1 ω7c (25.9%), C16:0 (23.3%), C17:0-cyclo (11.7%), C12:0 (6.0%) and C17:0 (5.9%). The genomic DNA G + C content of strain SG131T was 60.3%. The average nucleotide identity (ANI) values between strain SG131T and its most closely related species P. limicola DSM 6832T, P. pelophilus DSM 12018T and P. dicarboxylicus DSM 5885T were 74.4%, 74.9% and 75.6%, respectively. The digital DNA-DNA hybridization (dDDH) values between strain SG131T and its most closely related species P. limicola DSM 6832T, P. pelophilus DSM 12018T and P. dicarboxylicus DSM 5885T were 19.9%, 20.6% and 20.5%, respectively. All these values were lower than the recommended species delineation thresholds of ANI (95–96%) and dDDH (70%). Strain SG131T possessed core genes (nifHDK) of nitrogen fixation and was confirmed its nitrogen-fixing ability by the ARA method. According to the above-described analysis, strain SG131T represents a novel species of the genus Propionivibrio, for which the name Propionivibrio soli sp. nov. is proposed. The type strain is SG131T (= GDMCC 1.3313T = JCM 35595T).
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Data availability
The GenBank accession numbers for 16S rRNA gene and genome sequence of strain SG131T are OP345262 and JANZKY000000000, respectively.
Abbreviations
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA-DNA hybridization
- ARA:
-
Acetylene reduction activity
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Acknowledgements
We thank Prof. A. Oren for his kindly help and advice on nomenclature.
Funding
This work was supported by the National Natural Science Foundation of China (grant no: 41925028 and 42277257) and the Natural Science Foundation of Fujian Province (grant no: 2022G02014).
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SGZ and GHL designed the research and project outline. CJX and SY performed deposition and polyphasic taxonomy. RT performed isolation. SH performed genome analysis. CJX drafted the manuscript. SGZ, CR and GHL revised the manuscript. All authors read and approved the final manuscript.
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Xie, CJ., Tang, R., Yang, S. et al. A novel nitrogen-fixing bacterium, Propionivibrio soli sp. nov. isolated from paddy soil. Arch Microbiol 205, 68 (2023). https://doi.org/10.1007/s00203-023-03413-2
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DOI: https://doi.org/10.1007/s00203-023-03413-2