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Anti-hepatitis C virus and synergistic potential of Syzgium cumine a bioassay guided screening in liver-infected hepatocytes

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Abstract

Hepatitis C virus is still a global challenge affecting millions of carriers worldwide with the more devastating situation in developing countries. Present-day clinical manifestations are insufficient to tackle the increasing disease burden unaffordable cost, viral resistance and adverse effects of treatment. In this research, indigenous medicinal plants from Pakistan tested in bioassay guided manner on Huh-7 cell lines for their antiviral effect, synergism of purified fraction with interferon FDA approved drug regime, as the receptor for developing transfection model. The methanol extract of Syzgium cumine was observed against HCV through serum titter reduction in Quantitative Real Time PCR assay and the gene expression system, NS3protease inhibition was 76% and 51% against genotype 1a and 3a, respectively. More precisely the most active fraction SC14 was assessed in dose response assay and synergistic potential resulted in 50% reduction (EC50 Value) in HCV titer of genotype 1a and 3a at a concentration of 71.96 ± 8.67 µg and 31.75 ± 3.28 µg, respectively, at a concentration of 100 µg. As per our research work, the S. cumine extract has shown a promising effect on HCV genotypes 1a and 3a. Moreover the purified fraction S. cumine SC14 has a potential synergistic effect and ability to suppress the gene effect of NS3 during transfection in Huh-7 cells and GC/MS analysis reports the presence of Di-n-octyl phthalate (C24H38O4) which can be future direct-acting antiviral therapy against Hepatitis C virus.

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Acknowledgements

The current study of research work of PhD has been submitted in Higher Education Commission (HEC) of Pakistan by the author. This research was supported by Higher Education Commission, Pakistan.

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Authors and Affiliations

  1. Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan

    Sana Riaz

  2. Departments of Microbiology and Molecular Genetics, The Women University, Multan, Pakistan

    Sana Riaz

  3. Department of Biomedicine, Section Animal Physiology and Neurobiology, Department of Biology, KU, Leuven, Belgium

    Sana Riaz

  4. Basic Health Unit, Bhu Dhrah, Sargodha, Pakistan

    Hira Tiwana

  5. Department of Urology, Sheikh Zayed Hospital University, Avenue Muslim Town, Lahore, Pakistan

    Muhammad Adil

  6. Tehsil Headquarters Hospital, Muridqay, Sheikhupura, Pakistan

    Khaver Ali

  7. Department of Pharmaceutical Chemistry, Lahore Pharmacy College (LMDC) UHS, Lahore, Pakistan

    Tariq Javed

  8. Ruth Pfau College of Life Sciences (LMDC) GCU, Lahore, Pakistan

    Tariq Javed

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  1. Sana Riaz
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  2. Hira Tiwana
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  3. Muhammad Adil
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  4. Khaver Ali
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  5. Tariq Javed
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Contributions

TJ and HK designed the research, carried out most of the experiments MA and KA performed RNA extraction and Real Time PCR and collected HCV samples, SA wrote the manuscript and discussed with all the co-authors.

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Correspondence to Sana Riaz.

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All authors declare that they have no conflict of interest.

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Communicated by Ran Wang.

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Riaz, S., Tiwana, H., Adil, M. et al. Anti-hepatitis C virus and synergistic potential of Syzgium cumine a bioassay guided screening in liver-infected hepatocytes. Arch Microbiol 204, 69 (2022). https://doi.org/10.1007/s00203-021-02646-3

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  • DOI: https://doi.org/10.1007/s00203-021-02646-3

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