Abstract
Key message
We developed an efficient promoter editing method to create different weak Ehd1 alleles in elite japonica rice variety ZJ8 with slightly delayed heading and improved yield for use in breeding.
Abstract
Heading date is an important agronomic trait of rice (Oryza sativa) that determines the planting areas and cultivation seasons of different varieties, thus affecting final yield. Early heading date 1 (Ehd1) is a major rice integrator gene in the regulatory network of heading date whose expression level is negatively correlated with heading date and grain yield. Some elite japonica varieties such as Zhongjia 8 (ZJ8) show very early heading with poor agronomic traits when planted in South China. This problem can be addressed by downregulating the expression of Ehd1. In this study, we analyzed the cis-regulatory elements in the Ehd1 promoter region. We then used CRISPR/Cas9-mediated editing to modify the Ehd1 promoter at multiple target sites in ZJ8. We rapidly identified homozygous allelic mutations in the T2 generation via long-read sequencing. We obtained several Ehd1 promoter mutants with different degrees of lower Ehd1 expression, delayed heading date, and improved yield-related traits. We developed an efficient promoter editing method to create different weak Ehd1 alleles for breeding selection. Using this method, a series of heading date materials from elite varieties can be created to expand the planting area of rice and improve grain yields.
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Data supporting the findings of this work are available within the paper and its Supplementary Information files.
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Funding
This research was supported by grants from the National Nature Science Foundation of China (31871596, 31921004), the Major Program of Guangdong Basic and Applied Research (2019B030302006), and the Laboratory of Lingnan Modern Agriculture Project (NT2021002).
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JG, XX conceived the work, analyzed the data, and revised the manuscript. SL, YL, and GW performed the experiments and prepared the manuscript. WZ, DX, HZ, YS, and YH helped with the experiments. WZ helped analyze some of the data. KS, CL, XG, BX, WL and ZW helped investigate the phenotypes. JG, XX, and YL revised the manuscript.
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Li, S., Luo, Y., Wei, G. et al. Improving yield-related traits by editing the promoter of the heading date gene Ehd1 in rice. Theor Appl Genet 136, 239 (2023). https://doi.org/10.1007/s00122-023-04489-6
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DOI: https://doi.org/10.1007/s00122-023-04489-6