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Rpv10: a new locus from the Asian Vitis gene pool for pyramiding downy mildew resistance loci in grapevine

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Abstract

A population derived from a cross between grapevine breeding strain Gf.Ga-52-42 and cultivar ‘Solaris’ consisting of 265 F1-individuals was genetically mapped using SSR markers and screened for downy mildew resistance. Quantitative trait locus (QTL) analysis revealed two strong QTLs on linkage groups (LGs) 18 and 09. The locus on LG 18 was found to be identical with the previously described locus Rpv3 and is transmitted by Gf.Ga-52-42. ‘Solaris’ transmitted the resistance-related locus on LG 09 explaining up to 50% of the phenotypic variation in the population. This downy mildew resistance locus is named Rpv10 for resistance to Plasmopara viticola. Rpv10 was initially introgressed from Vitis amurensis, a wild species of the Asian Vitis gene pool. The one-LOD supported confidence interval of the QTL spans a section of 2.1 centi Morgan (cM) corresponding to 314 kb in the reference genome PN40024 (12x). Eight resistance gene analogues (RGAs) of the NBS–LRR type and additional resistance-linked genes are located in this region of PN40024. The F1 sub-population which contains the Rpv3 as well as the Rpv10 locus showed a significantly higher degree of resistance, indicating additive effects by pyramiding of resistance loci. Possibilities for using the resistance locus Rpv10 in a grapevine breeding programme are discussed. Furthermore, the marker data revealed ‘Severnyi’ × ‘Muscat Ottonel’ as the true parentage for the male parent of ‘Solaris’.

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Acknowledgments

This project was funded by the “Forschungsring des Deutschen Weinbaus (FDW)”. We thank Erika Maul for the ampelographical support in the pedigree analysis and Friederike Rex for fruitful discussions.

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  1. JKI Institute for Grapevine Breeding Geilweilerhof, 76833, Siebeldingen, Germany

    Florian Schwander, Rudolf Eibach, Iris Fechter, Ludger Hausmann, Eva Zyprian & Reinhard Töpfer

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  1. Florian Schwander
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Correspondence to Florian Schwander.

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122_2011_1695_MOESM2_ESM.emf

Suppl. Fig 1 a) Pedigree of `Solaris´ confirmed by the marker analysis. b) Currently assumed pedigree version (Staatliches Weinbauinstitut Freiburg 2010) and c) originally given pedigree version for `Solaris´ (Becker 1981). Continuous lines indicate SSR marker-confirmed linkages between tested cultivars (continuous boxes). Cultivars in dashed boxes and dashed lines are related predecessors according to the “Vitis International Variety Catalogue” (http://www.vivc.de). Dark grey represents presence of Rpv10, for `Saperavi Severnyi´ the presence or absence of Rpv10 is unknown. (EMF 365 kb)

122_2011_1695_MOESM3_ESM.emf

Suppl. Fig 2 Examples for SSR-Markers used for excluding `Zarya Severa´ as an ancestor of `Solaris´ (a, b) and confirming `Severnyi´ (a, b) and `Muscat Ottonel´ (c) as true ancestors. Observed fragment lengths (bp) are given for both alleles below the variety names. a) `Solaris´ inherited the allele with the 120 bp fragment from `Merzling´ which was confirmed as the maternal parent of `Solaris´ with all used markers. DNA of the pollen donor Geisenheim 6493 is not available because the name stands for a pollen mixture of a cross population. Thus, the 102 bp fragment of `Solaris´ must be found in one of the grandparents. In this case it is present in `Severnyi´. The absence in `Zarya Severa´ excludes this variety as an ancestor. b) Identical situation as demonstated in a) of a marker in the Rpv10 region. c) Example for a marker confirming `Muscat Ottonel´ as a grandparent of `Solaris´. `Saperavi severnyi´ can be excluded without testing its DNA by the observation that all alleles could be traced back to either `Severnyi´ or `Muscat Ottonel´. If `Saperavi severnyi´ was involved, up to ¼ (in case of only heterozygous markers) of the alleles (the ones going back to `Saperavi´) in `Solaris´ would not match. (EMF 181 kb)

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Schwander, F., Eibach, R., Fechter, I. et al. Rpv10: a new locus from the Asian Vitis gene pool for pyramiding downy mildew resistance loci in grapevine. Theor Appl Genet 124, 163–176 (2012). https://doi.org/10.1007/s00122-011-1695-4

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