Relaxation-Enhanced Angiography Without Contrast and Triggering (REACT) for Fast Imaging of Extracranial Arteries in Acute Ischemic Stroke at 3 T

Purpose To evaluate a novel flow-independent 3D isotropic REACT sequence compared with CE-MRA for the imaging of extracranial arteries in acute ischemic stroke (AIS). Methods This was a retrospective study of 35 patients who underwent a stroke protocol at 3 T including REACT (fixed scan time: 2:46 min) and CE-MRA of the extracranial arteries. Three radiologists evaluated scans regarding vessel delineation, signal, and contrast and assessed overall image noise and artifacts using 5-point scales (5: excellent delineation/no artifacts). Apparent signal- and contrast-to-noise ratios (aSNR/aCNR) were measured for the common carotid artery (CCA), internal carotid artery (ICA, C1 segment), and vertebral artery (V2 segment). Two radiologists graded the degree of proximal ICA stenosis. Results Compared to REACT, CE-MRA showed better delineation for the CCA and ICA (C1 and C2 segments) (median 5, range 2–5 vs. 4, range 3–5; P < 0.05). For the ICA (C1 and C2 segments), REACT provided a higher signal (5, range 3–5; P < 0.05/4.5, range 3–5; P > 0.05 vs. 4, range 2–5) and contrast (5, range 3–5 vs. 4, range 2–5; P > 0.05) than CE-MRA. The remaining segments of the blood-supplying vessels showed equal medians. There was no significant difference regarding artifacts, whereas REACT provided significantly lower image noise (4, range 3–5 vs. 4 range 2–5; P < 0.05) with a higher aSNR (P < 0.05) and aCNR (P < 0.05) for all vessels combined. For clinically relevant (≥50%) ICA stenosis, REACT achieved a detection sensitivity of 93.75% and a specificity of 100%. Conclusion Given its fast acquisition, comparable image quality to CE-MRA and high sensitivity and specificity for the detection of ICA stenosis, REACT was proven to be a clinically applicable method to assess extracranial arteries in AIS. Electronic supplementary material The online version of this article (10.1007/s00062-020-00963-6) contains supplementary material, which is available to authorized users.


Introduction
In acute ischemic stroke (AIS), imaging of the extracranial arteries is required to detect atherosclerosis and associated comorbidities, such as internal carotid artery (ICA) stenosis as well as large vessel occlusion and dissection [1].
First-pass contrast-enhanced magnetic resonance angiography (CE-MRA) using gadolinium-based contrast agents represents the standard of care for cervical arteries in stroke magnetic resonance imaging (MRI), providing high spatial resolution [2,3]. However, CE-MRA shows limitations regarding the potential side effects of contrast agents, such as allergic reactions, nephrogenic systemic fibrosis in end-stage renal disease, and uncertain longterm effects of gadolinium deposition in the brain [4][5][6][7][8].
Occasionally, mistiming of image acquisition regarding first-pass contrast bolus resulting in insufficient contrast or venous contamination leads to impaired image quality in CE-MRA [9,10]. In AIS, contrast agents might be reserved for perfusion MRI using first-pass dynamic imaging techniques [11].
Hence, several non-CE-MRA techniques have been developed in the past, with 2D/3D time-of-flight (TOF)-MRA being a possible approach for extracranial arteries; however, compared to CE-MRA, 2D/3D TOF-MRA has the disadvantages of a long acquisition time, sensitivity to respiratory and flow artifacts, inferior image quality, decreased anatomic coverage and overestimation of ICA stenosis [12][13][14][15][16]. Beyond TOF-MRA, different non-CE-MRA techniques have been recently proposed, with quiescent interval slice-selective (QISS)-MRA and its flowcompensated fast low-angle shot readout being one of the latest innovations to show promising results for different vascular territories, including extracranial and intracranial arteries [10,[17][18][19][20]. However, 2D acquisition and dependency on the inflow of spins from outside the saturation volume have to be considered [10,[18][19][20].
Recently, a novel 3D Relaxation-Enhanced Angiography without Contrast and Triggering (REACT) sequence, a combination of non-volume-selective short tau inversion recovery (STIR) and T2 preparation pulses with dual gradient echo Dixon (mDIXON XD) readout, was introduced. It combines the benefits of steady-state free precession (SSFP), such as bright-blood signal with robust fat and background suppression for flow-independent isotropic 3D non-CE-MRA [21]. While it is not suitable for intracranial MRA, REACT provides a simultaneous depiction of arterial and venous vessels and has shown encouraging results in displaying the pulmonary vasculature in congenital heart disease at 1.5 T [21,22].
The purpose of this study was to compare the image quality of extracranial arteries and the assessment of ICA stenosis between REACT and CE-MRA at 3 T in patients with AIS.

Material and Methods
The institutional review board approved this retrospective, single-center study (reference number:  and waived the need for written informed consent from the patient cohort.

Patient Population
We retrospectively reviewed our internal database for stroke MRI studies from May to July 2019. Scans were included if patients underwent a standard protocol for AIS at 3 T in clinical routine with both REACT and CE-MRA sequences depicting the extracranial arteries. Severe motion artifacts or pronounced pleural effusions led to patient exclusion. For non-CE-MRA, imaging was based on a flow-independent 3D isotropic REACT sequence combining a 50 ms T2 preparation sequence and a STIR pulse with a 3D mDIXON XD (Philips Healthcare) readout [21]. The combination of T2 preparation and STIR enables the suppression of tissue with short T1 and T2, while enhancing the blood signal with long T1 and T2. For fat suppression, the mDIXON XD technique is applied. Since REACT is based on relaxation times, it can be used without any form of triggering, with data being acquired in the coronal plane. Compressed SENSE (Philips Healthcare) was used for the acceleration of image acquisition, a method providing a combination of compressed sensing and parallel imaging using SENSitivity Encoding (SENSE) [23][24][25]. An acceleration factor of 4 was employed, resulting in a fixed scan time of 2:46 min. Immediate image reconstruction was used. Given the known fat-water swapping artifacts of the mDIXON XD readout, water-only as well as in-phase and out-of-phase reconstructions were created [26][27][28].

K
For CE-MRA, a 3D spoiled gradient-echo T1 sequence was used. Serving as a mask, a native MRA image was acquired. Gadoteric acid (Clariscan, GE Healthcare, Chicago, IL, USA; 0.2 ml/kg body weight) was automatically injected into an antecubital vein at a flow rate of 2 ml/s, followed by a 30 ml saline flush. Without any triggering, acquisition in the coronal plane was started by the arrival of contrast agent in the aortic arch, as determined by a bolus tracking sequence. No table movement was conducted between bolus tracking and acquisition of the T1 sequence. No subtraction of the CE-MRA images from the native scan was performed. A Compressed SENSE factor of 6 was used, resulting in a nominal scan time of 1:08 min. Real time reconstruction was employed. Table 1 summarizes the imaging parameters of the RE-ACT and CE-MRA sequences. For REACT and CE-MRA, the time was noted from the beginning of acquisition until image reconstruction was completed.

Subjective Evaluation of Image Quality
Three readers with different levels of expertise in MRA (two radiologists each with 4 years of experience and one board-certified neuroradiologist with 14 years of experience) independently evaluated the MRA datasets in random order during separate sessions. Source images and maximum intensity projections (MIPs) in the coronal plane for both techniques (water-only for REACT; slice thickness of 6 mm, gap of 0 mm) were analyzed using the same IMPAX EE (Agfa HealthCare N.V., Mortsel, Belgium) workstation. Readers were aware of potential fat-water swapping artifacts in REACT and were free to choose among respective reconstructions of its source images.
The evaluation of vessel image quality was based on three distinct criteria: vessel delineation, the vessel signal, and vessel contrast to the surrounding tissue. For each criterion, a scoring scale of 1-5 was used: 1. non-diagnostic, image quality inadequate for diagnosis 2. poor, suboptimal image quality for diagnosis 3. fair, moderate image quality acceptable for diagnosis 4. good, image quality suitable for confident diagnosis 5. excellent, image quality providing highly confident diagnosis Vessel quality was scored for the following 9 segments: 1. aortic arch/adjacent branches 2. bilateral common carotid artery (CCA) 3. bilateral ICA in the cervical (C1) segment 4. bilateral ICA in the petrous (C2) segment 5. bilateral proximal external carotid artery (ECA) 6. bilateral distal ECA (parotid space) 7. bilateral vertebral artery (V1 segment) 8. bilateral vertebral artery (V2 segment) 9. bilateral vertebral artery (V3 segment) Additionally, investigators rated the overall presence of artifacts (blurring artifacts, banding artifacts, pulsation artifacts, and parallel imaging reconstruction artifacts) and the overall image noise with the following 5-point scoring system: 1 non-diagnostic, 2 high impact, 3 moderate impact, 4 low impact, and 5 none.

Objective Evaluation of Image Quality
One radiologist with 4 years of experience in MRA conducted apparent SNR (aSNR) and apparent contrast-tonoise ratio (aCNR) measurements by drawing the region of interest (ROI) in the same position on source images from REACT (water-only) and CE-MRA in the following vessels: 1. Right and left CCAs (3 cm proximal to the carotid bifurcation) 2. Right and left C1 segments of the ICA (3 cm distal to the carotid bifurcation) 3. Right and left V2 segments of the vertebral artery (4 cm distal to the transverse foramen of the sixth vertebra) As a reference standard for background noise, an ROI was placed on the adjacent sternocleidomastoid muscle ipsilateral to the respective vessel. This intracorporeal, homogeneous tissue located close to the signal measurement was chosen as the reference standard, given the masking effect of the extracorporeal background during image reconstruction.
The aSNR and aCNR were calculated as follows: aSNR = SI vessel SD of SI muscle aCNR = .SI vessel − SI muscle /

SD of SI muscle
where SI is the signal intensity, and SD is the standard deviation. For each segment, the mean value of the aSNR and aCNR for both sides was analyzed.

Presence of Fat-water Swapping Artifacts
One radiologist with 4 years of experience in MRA assessed the acquired water maps from REACT for the presence of fat-water swapping artifacts and the corresponding signal of the in-phase image at the respective signal loss of the water map.

Grading of Proximal ICA Stenosis
Two radiologists with different levels of expertise in MRA, 1 radiologist with 3 years and 1 board-certified neuroradiologist with 13 years of experience, independently assessed the aforementioned source images and MIPs of the extracranial MRA techniques for proximal ICA stenosis in random order during separate reading sessions. Using the same IM-PAX EE (Agfa HealthCare N.V.) workstation, the following grading scale was applied: Grade 1: normal patency Grade 2: <50% stenosis Grade 3: 50-69% stenosis Grade 4: ≥70-99% stenosis Grade 5: occlusion

Statistical Analysis
Statistical analysis was performed using JMP (release 14.1.0, SAS Institute, Cary, NC, USA), with the statistical significance set to P < 0.05. Quantitative measurements are indicated as the mean ± standard deviation, unless noted otherwise. The subjective image quality evaluation data are presented as medians with minimum and maximum values. To compare quantitative values and subjective scores, the Wilcoxon rank-sum test was used. The sensitivity and specificity of REACT for ICA stenosis were calculated considering CE-MRA as a reference standard since the latter provides high diagnostic accuracy [29,30]. In cases of disagreement regarding the reference standard, a consensus diagnosis was established. A stenosis grade ≥50% was considered clinically relevant. Interobserver agreement for the subjective evaluation of image quality was assessed using Kendall's coefficient of concordance (Kendall's W). Regarding the assessment of ICA stenosis, Cohen's Kappa was used to evaluate the interobserver agreement of REACT and the intersequence agreement between REACT and CE-MRA considering the disease grade. The interpretation of agreement was as follows: K 0.01-0.2 slight, 0.21-0.4 fair, 0.41-0.6 moderate, 0.61-0.8 substantial, and 0.81-0.99 almost perfect agreement [31].

Study Population and Baseline Characteristics
A total of 40 patients were identified, of whom 2 had to be excluded due to severe motion artifacts in both sequences and 1 each due to artifacts in either REACT or CE-MRA, respectively. Another patient was excluded due to severe pleural effusion leading to hampered image quality in REACT. Consequently, 35 patients were included in this study (mean age 60.3 ± 21.0 years, 17 females, range 13-86 years).

Imaging
REACT had a fixed total acquisition time of 2:46 min. With respect to Compressed SENSE reconstruction and acquired mDIXON XD images, this amounted to an average

Subjective Evaluation of Image Quality
Three readers each evaluated 70 datasets (35 datasets each for REACT and CE-MRA), resulting in a total number of 630 analyzed arterial segments. Table 1 of the supplementary material provides the image quality scores for each imaging technique and the arterial segments regarding vessel delineation, signal, and contrast to the surrounding tissue.
Regarding vessel signal and contrast, REACT provided superior results to CE-MRA with a higher median for signal (C1 segment: 5, range 3-5 vs.   Effect of noise on image quality in an 82-year-old male patient with embolic ischemia of the right precentral gyrus as shown in coronal maximum intensity projections (slice thickness: 20 mm). In Relaxation-Enhanced Angiography without Contrast and Triggering (RE-ACT, water-only), the V1 segments (thin arrows) can be sufficiently delineated, whereas image noise leads to a blurred appearance in contrast-enhanced magnetic resonance angiography (CE-MRA). The image quality of the remaining segments of the vertebral arteries is comparable between both techniques. Note the insufficiency of REACT to differentiate the V4 segments from cerebrospinal fluid lower minimum scores than REACT for vessel contrast (Fig. 2).
Regarding the overall distribution of scores for all readers combined, Table 2 provides the detailed results. RE-ACT achieved higher values for delineation, signal, and contrast for blood-supplying vessels (BSVs; aortic arch/ adjacent branches, CCA, C1 and C2 segments of the ICA, and V1-V3 segments) than for all vessels combined. CE-MRA showed that the distribution of scores was widely independent of vascular regions. For BSVs, REACT achieved good to excellent scores of 81% for delineation, 87% for signal, and 89% for contrast. CE-MRA reached good to excellent scores of 91%, 89%, and 88% of cases for these criteria, respectively. REACT provided a lower number of poor scores for these vessels than CE-MRA.
There was no significant difference in the overall presence of image artifacts (REACT: 4, 3-5; CE-MRA: 4, 3-5; P = 0.214) (Fig. 2). REACT showed a significantly lower overall image noise than CE-MRA (4, 3-5 vs. 4, 2-5; P < 0.001) (Figs. 2 and 3). This difference is due to the fact that readers scored a fraction of scans from CE-MRA with high impact image noise (score of 2), whereas in the worst cases of REACT, image noise was only rated to have moderate impact (score of 3).

Interobserver Agreement for Subjective Image Quality in REACT and CE-MRA
The results of interobserver agreement are outlined in Table 2 of the supplementary material. There was moderate agreement (Kendall's W > 0.41) for all vessel quality criteria for both methods of imaging and for noise in CE-MRA, whereas the assessment of noise in REACT and artifacts in both MRA techniques demonstrated fair agreement (Kendall's W > 0.21). Table 3 provides the detailed results regarding the aSNR and aCNR for each imaging modality and segment. REACT achieved higher aSNR and aCNR values at all analyzed segments, reaching statistical significance for the CCA and vertebral artery, whereas at the C1 segment of the ICA, no significant difference was noted (aSNR: P = 0.151, aCNR: P = 0.34). When combining all measurements, REACT pro-

Presence of Fat-water Swapping Artifacts
In 10 of 35 patients, fat-water swapping artifacts in REACT affecting the left subclavian artery (10 cases) and the left CCA (one case) were noted. Corresponding to the signal loss of water-only images, in-phase images provided a high vessel signal in every case (Fig. 2 of the supplementary material).

Grading of Proximal ICA Stenosis
Considering CE-MRA as a reference standard, REACT achieved a sensitivity of 90% and a specificity of 98.34% for any stenosis by both readers. Considering clinically relevant stenosis (≥50%), REACT provided a sensitivity of 93.75% with a corresponding specificity of 100%. There was almost perfect agreement for REACT between both readers (Cohen's Kappa of 0.89). Furthermore, REACT achieved almost perfect agreement with CE-MRA regarding the disease grade (Cohen's Kappa of 0.86) (Figs. 5 and 6). In both sequences, the two readers graded the stenosis as grade 4

Discussion
In this study, we assessed a novel REACT sequence for imaging of extracranial arteries at 3 T in patients with AIS in clinical routine by comparing the image quality and grading of ICA stenosis in REACT with those in CE-MRA.
The major findings of the study are as follows: 1. In less than 3 minutes, REACT provided comparable image quality of the extracranial arteries in AIS to CE-MRA, without the use of triggering or gadolinium-based contrast agents. 2. Albeit showing inferior vessel delineation compared to CE-MRA, REACT achieved equal if not higher vessel signal and contrast for the BSVs as well as lower overall image noise outlined by subjective and objective results. 3. RE-ACT provided high sensitivity and specificity for the detection of ICA stenosis.
In line with previous studies comparing non-CE-MRA techniques such as TOF-MRA and QISS-MRA with CE-MRA, REACT did not fully reach the exact level of image quality of high-resolution CE-MRA with inferior vessel delineation of the carotid arteries [10,18,19]. A possible showing an internal carotid artery stenosis (wide arrows) in Relaxation-Enhanced Angiography without Contrast and Triggering (REACT, water-only) and contrast-enhanced magnetic resonance angiography (CE-MRA). In both sequences, the two readers graded the stenosis as grade 3. Note the effect of image noise and pulsation artifacts on the proximal common carotid artery and V1 segment of the vertebral artery in CE-MRA, leading to impaired vessel quality (thin arrows) explanation for this finding might be the slightly inferior resolution of REACT and the longer acquisition time than CE-MRA, which might impact image sharpness, e.g., due to blurring caused by physiological motion. However, the quality of the BSVs, especially regarding signal and contrast, was graded as good or excellent in the majority of cases and was pronounced at the carotid arteries. Furthermore, the subjectively described higher signal and contrast for the majority of these vessels, especially for the carotid arteries, as well as the lower overall image noise of RE-ACT than of CE-MRA, were confirmed by objective results showing significantly higher aSNR and aCNR values for the non-CE-MRA technique. In addition to the technical differences between both MRA techniques, the higher aSNR and aCNR values with REACT may be explained by the longer acquisition time, subsequently leading to reduced noise. REACT revealed limitations in the depiction of the ECA with minor clinical relevance in the setting of AIS. Contrary to the results in carotid arteries, REACT provided equal vessel delineation of the aortic arch/adjacent branches to CE-MRA, which was occasionally hampered by pulsation artifacts. For the BSVs, REACT provided more balanced results with a lower number of segments being graded as poor quality than CE-MRA.
Considering CE-MRA as a reference standard, REACT achieved a high sensitivity (90%) and specificity (98%) for the detection of proximal ICA stenosis, especially for clinically relevant stenosis (94% and 100%, respectively). In the literature, these findings are comparable to the reported sensitivity (up to 86%) and specificity (up to 90%) of QISS-MRA for proximal ICA stenosis [10,19]. While TOF-MRA tends to overestimate proximal ICA stenosis [14][15][16], almost perfect agreement was observed between REACT and CE-MRA (as reported for QISS-MRA [18,19]) considering the disease grade, indicating the clinical potential of RE-ACT to sufficiently detect and grade ICA stenosis without the use of gadolinium-based contrast agents.
Given the aforementioned limitations of CE-MRA, non-CE-MRA techniques are of particular interest in research and clinical practice [32,33]. Despite its easy implementation and high spatial resolution, TOF-MRA is generally considered insufficient for imaging extracranial arteries due to its flow dependency and the long acquisition time needed to cover a large field of view as well as because its image quality is limited to the presence of horizontally directed vessel segments [12,13,32,34]. Hence, there is a need for non-CE-MRA techniques yielding high image quality with a short scan time.
QISS-MRA provides a promising approach with its fast low-angle shot read-out leading to reduced sensitivity to offresonance effects and the use of in-plane inversion allowing for adequate background suppression and high arterial-tobackground contrast [10,18,19]. However, there are potential drawbacks due to the 2D acquisition of QISS-MRA with dependency on the inflow of spins from outside the saturation volume, which is associated with technical limitations such as anisotropic image volumes, long acquisition times, blood flow dependency, and vessel orientation relative to the imaging slices [10,18,19]. In contrast, the mDIXON XD read-out of REACT combines the known benefits of SSFP with the robust suppression of fat and background as well as the separation of water and fat, consequently leading to an insensitivity to inhomogeneities in the magnetic field and providing high-resolution 3D scans in a large field of view [21,26,35].
With the introduction of new acceleration techniques, such as compressed sensing, shorter acquisition times beyond those of the current parallel imaging techniques are feasible, especially when combining both techniques [23][24][25]. In this work, Compressed SENSE, which has already shown encouraging results in musculoskeletal and cardiovascular imaging, was fully integrated into the clinical system, providing image acquisition acceleration currently not achievable by compressed sensing or parallel imaging alone [22-25, 36, 37]. Given the fast acquisition time of the scan itself (less than 3 minutes) and the short reconstruction times (less than 4 minutes from the beginning of the scan to complete image reconstruction), REACT was proven to be clinically applicable in routine emergency imaging.
REACT is faster to acquire than not only QISS-MRA (scan time of up to 7 minutes) and TOF-MRA but also the CE-MRA sequence used in this study [10,19]. Albeit having an acquisition time of approximately 1:45 minutes (when including the native scan (~5 s) and the bolustracking sequence (~30 s, depending on the patient's circulation)) and a combined acquisition and reconstruction time of 3 minutes, the total time to perform CE-MRA is in fact longer than that to perform REACT when including the time needed for preparation of the patient for contrast injection. With the image quality of CE-MRA occasionally impaired due to mistiming between bolus application and data acquisition, REACT is proven to be widely user-independent and may be repeated as often as required without repetitive application of contrast agent. This is of particular relevance for patients requiring follow-up imaging [9,10]. From an economic point of view, abandonment of contrast agents might reduce the cost of stroke MRI and facilitate clinical workflows.
Compared to 2D acquisition of QISS-MRA, REACT, similar to TOF-MRA, is able to provide an isotropic 3D readout enabling image reconstruction in all three directions of space [10,18,19]. The image quality of TOF-MRA and QISS-MRA may be hampered due to the dependency on blood flow and the associated sensitivity to flow artifacts [10,12,13,18,19,32,34]. In contrast, REACT exploits the specific relaxation properties of blood and is therefore independent of blood flow and can be acquired without any triggering, hence facilitating its use in clinical routine since head and neck imaging is generally performed without cardiac or pulse triggering [21]. Given the sufficient background suppression of the REACT sequence, an image-based navigator used to reduce swallowing motion artifacts as reported in a recent QISS-MRA study at 1.5 T is not needed [10]. Similar to TOF-MRA and QISS-MRA, REACT is acquired during free-breathing and is therefore suitable for patients unable to hold their breath [21].
The fat-water swapping artifacts of the mDIXON XD read-out with intermittent vessel signal loss in the wateronly images in 10 of 35 patients may suggest a drawback of REACT [26][27][28]. Thus, future investigations are warranted; however, the corresponding in-phase images provided high signal for the respective vessel in each patient, clarifying the drop-out as an artifact. Whereas QISS-MRA and TOF-MRA provide scans with low or no venous contamination, the (inferior) signals of the venous vasculature and of perivascular structures may be regarded as a limita-tion of REACT potentially causing a crowded image [10,18,19,21]. The vessel signal of REACT is T2-weighted and depends on the O2 saturation, leading to a higher signal and contrast of the arterial vasculature, thus enabling sufficient differentiation of arteries, veins, and adjacent soft tissues [21]. In this context, we chose a low flip angle (15°) to provide a high arterial signal. Given the selectivity of REACT for tissues with long T1 and T2, the image quality of REACT is decreased in patients with severe pleural effusions, which are not known to influence the image quality of QISS-MRA and TOF-MRA. In comparison to QISS-MRA and TOF-MRA, REACT is unable to display intracranial arteries given the high signal of cerebrospinal fluid, which also has long T1 and T2 [20,21,38]. This needs to be recognized as an important limitation of the sequence. Nevertheless, 3D TOF-MRA has been established as a highly reliable and standard non-CE-MRA technique for cerebral vessels with the combination of both techniques, allowing for the sufficient display of extracranial and intracranial arteries in the setting of AIS without gadolinium-based contrast agents [32,38].

Limitations
In addition to being a retrospective single center investigation, our study has some limitations. We did not compare REACT to digital subtraction angiography (DSA), which is considered the gold standard for imaging of extracranial and intracranial arteries [39]. The measurement of the SNR and CNR in REACT (Compressed SENSE factor 4) and CE-MRA (Compressed SENSE factor 6) may be regarded as a drawback of this study design since techniques such as parallel imaging may influence the true values of the SNR and CNR [40,41]. However, to verify the subjective ratings of vessel signal and contrast as well as noise (and given the small difference among Compressed SENSE factors), we chose to measure the apparent values of the SNR and CNR to provide an objective evaluation of vessel signal and contrast as well as noise, with the objective results being in line with the subjective scores. Regarding the grading of ICA stenosis, a larger patient cohort may be needed to confirm the results of this study as well as to further investigate whether REACT can detect atherosclerotic plaques sufficiently and evaluate their morphology. No direct comparison to other non-CE-MRA sequences, such as QISS-MRA or TOF-MRA, was conducted in this study, which could be addressed in future investigations.

Conclusion
This initial study indicates that REACT is suitable for routine emergency imaging, allowing fast depiction of extracra-nial arteries in AIS with image quality comparable to highresolution CE-MRA as well as high detection sensitivity for ICA stenosis.