Individual dental and skeletal age assessment according to Demirjian and Baccetti: Updated norm values for Central-European patients

Purpose Chronological age often differs from dental and skeletal age. With orthopantomograms and lateral cephalograms, dental and skeletal development can be determined according to the methods published by Demirjian et al. and Baccetti et al. However, gender and skeletal class as possible confounders were frequently not considered and available norm values are not up-to-date. This retrospective cross-sectional study thus aimed to evaluate effects of skeletal class and gender on dental and skeletal age of growing patients and to generate updated norm values for contemporary Central-European patients. Methods A total of 551 patients were included in the dental and 733 in the skeletal age assessment, respectively. Dental analysis was based on tooth mineralisation stages in orthopantomograms (Demirjian) and skeletal age was defined by cervical vertebrae maturation stages (CVMS) in lateral cephalograms (Baccetti). Skeletal class was determined by the individualised ANB angle of Panagiotidis/Witt. With nonlinear regression analysis a formula for determining dental age was established. Effects of gender and skeletal class were evaluated and updated norm values generated. Results Inter- and intrarater reliability tests revealed at least substantial measurement concordance for tooth mineralisation and CVMS. Demirjian stages and CVMS significantly depended on gender with girls developing earlier. Skeletal class significantly affected skeletal age only, but without clinical relevance. Updated norm values for dental age differed significantly from the original values of Demirjian and the values for skeletal age differed from those published by Baccetti. Conclusion Optimised norms, separated by gender, increase precision in determining individual dental and skeletal age during orthodontic treatment planning. Further studies analysing the effect of skeletal class on dental and skeletal development are needed.


Introduction
Orthodontic decision-making requires knowledge about dental and skeletal development to optimise timing of orthodontic treatment in dependence of biological age [51].Skeletal maturity provides information about expected growth potential, which is necessary for orthopaedic effects, and dental age acts as a first (clinical) evaluation of an individual's developmental stage during initial diagnostics.
Several methods exist to determine an individual's biological age [29] as opposed to the patient's actual chronological age, among which radiographic imaging is a major contributing factor for dental and skeletal age assessment.Whereas specific radiologic imaging such as hand-wrist radiographs result in additional radiation and require strict indications, orthopantomograms and lateral cephalograms are readily available as part of routine diagnostics throughout orthodontic treatment.
In 1972, Lamparski [31] determined skeletal maturity in lateral cephalograms by evaluating changes in shape, size and ratios of cervical vertebrae and distinguished between six grades, which generally occurred earlier in girls than in boys.This idea was further developed by Hassel and Farman [20], who established six cervical vertebral maturation (CVM) indicators, based on growth-related changes in C2, C3 and C4 which were associated with growth potential, as a comparison with hand-wrist radiographs showed.Baccetti et al. [4] optimised this technique by analysing the same cervical vertebrae in six consecutive lateral cephalograms of 30 patients and published five CVM stages (CVMS).Although in literature contradictory information exists, several studies concluded that lateral cephalograms are often equally suitable as hand-wrist radiographs to determine skeletal age [18,37,46].
Teeth were found to erupt within certain chronological time intervals, enabling the clinician to assess dental age by comparing the individual tooth status.However, as there is a range of 2 years in forecasting tooth eruption [43], the clinical prediction of dental age is imprecise und not appropriate to establish a treatment plan.In the 1940s, Schour and Massler [53,54] discovered the continuity of dental development.Their atlas method, a comparison between schematic graphs and individual dental developmental stages in orthopantomograms [54], is still used today, although other methods also exist to assess dental age [40,52].Demirjian et al. [12] analysed mineralisation stages of teeth in the third quadrant using orthopantomograms to define dental age, separated by gender.
Sagittal dysgnathias are subdivided into three groups: normal relationship between the upper and lower jaw (skeletal class I), distobasal jaw relation (skeletal class II) and mesiobasal jaw relation (skeletal class III).A patient's skeletal class can be determined by several parameters in cephalometric analysis, but floating norms, considering vertical and sagittal variables and thus the influence of vertical jaw development on sagittal changes, proved to be reliable and valid [55].One method used to individualise the ANB angle is the regression equation of Panagiotidis and Witt [45].
In the literature contradictory information about a possible association between the skeletal class and dental/ skeletal age has been published.Although some authors [9] found a correlation between dental age and sagittal dysgnathia, others [24] described a relationship between dental age and vertical dysgnathia, which was not confirmed by Jamroz et al. [23].Morphology of the cervical vertebrae, used as an indicator of skeletal development, seems to be influenced by skeletal class in adult patients [58].A re- cently published study reported skeletal class-and genderrelated differences in skeletal age, although the clinical relevance was rather small [48].The methods of Demirjian et al. and Baccetti et al. to determine dental and skeletal age, respectively, are generally accepted, but there are some limitations.Furthermore previous studies have questioned the reliability of the techniques of Demirjian et al. [19] and Baccetti et al. [17].Both are based on a population different from contemporary Central-European patients and do not consider skeletal class as a possible confounding variable.Furthermore, the CVMS method of Baccetti et al. did not investigate gender-related differences in skeletal maturation and relied on a small sample (n = 30).
Therefore, the aim of this study was primarily to assess the association between dental and skeletal age, using the methods of Demirjian [12] and Baccetti [4], respectively, on the one hand, and gender and skeletal class on the other hand in growing orthodontic patients.Secondly, updated norm values for dental and skeletal age for a contemporary Central-European population should be generated.

Materials and methods
In this retrospective cross-sectional study orthopantomograms and lateral cephalograms of 400 consecutively selected patients per skeletal class (I, II and III, n = 1200 in total) were screened for eligibility.Due to the multicentric study design, patients were recruited at two different specialist orthodontic practices in Germany (Bavaria and Baden-Wuerttemberg).Only those radiographs taken for diagnostic purposes between 1993 and 2013 were considered.For each patient and time (before, during or after treatment) gender and chronological age were recorded.Ethnicity, a potential confounder, was not documented, since comparable ethnic groups were assumed due to the exclusively German study locations.Orthopantomograms were used to assess dental age, whereas lateral cephalograms were evaluated for the determination of skeletal age and skeletal class.The study was performed in concordance with the Declaration of Helsinki (2013), the ethical guidelines of the University of Regensburg (approval of the ethical committee: 19-1596-101) and the Strengthening the Reporting of Observational studies in Epidemiology (STROBE) guideline.
To avoid distortion of the data, patients were not eligible for this study if systemic, local, exogenous, iatrogenic or genetical factors could have affected dental or skeletal development or if this maturation was not clearly assessable.Moreover, no patients younger than 6 or older than 20 years were considered because radiographs at these chronological ages were scarce and would not allow meaningful statistics.Also those patients who did not have a lateral cephalogram and orthopantomogram taken within 3 months at initial, interim or final examination were excluded to enable statistical testing of both dental and skeletal parameters.Finally, poor quality radiographs, hindering analysis of the predefined outcomes (mineralisation stage, CVMS, individualised ANB, skeletal class) led to exclusion.Patients with 100 Demirjian points (meaning completion of root development) and CVMS V were excluded from further analyses, since these stages are not assigned to a specific dental/skeletal age and could introduce bias into the statistics.Finally, 551 patients (265 male, 286 female) were recruited for dental age assessment and 733 patients (374 male, 359 female) for determination of skeletal maturation (Fig. 1).All participants were anonymised and randomised directly at the source.For further analyses, patients were subdivided in four chronological age groups (≤ 10 years, 10-12 years, 12-14 years, > 14 years).
Dental and skeletal age were analysed by one rater (AD) under blinding of the chronological age using the orthopantomograms and lateral cephalograms at a randomised time, i.e. initial, interim or final examination.Cephalometric analysis covered only measurement of the individualised ANB angle of Panagiotidis and Witt [45] to determine the skeletal class.If analysis could not be performed at the randomly chosen timepoint, another date was randomly selected.
Within the time interval from 1993-2013 the radiographic devices were switched from analogue to digital in both orthodontic offices.Analogue images were taken with the device OrthOralix FD ceph (Philips Gendex Dental-Systeme, Hamburg, Germany) and digital radiographs with the device Veraviewepocs 2D (J.Morita Europe GmbH, Dietzenbach, Germany).The following settings were applied for the orthopantomograms: anode voltage of 63-69 and 62-69 kV, anode current strength of 6.0 and 5.3-7.3mA and an exposure time of 17 and 14.8 s in analogue and digital images, respectively.Lateral cephalograms were conducted with an anode voltage of 69-73 and 70-80 kV, an anode current strength of 6.0 and 7.0-7.2mA and an exposure time of 12 and 4.9 s in analogue and digital radiographs, respectively.
Skeletal class was defined by comparing measured and individualised ANB angle of Panagiotidis and Witt [45], which was calculated applying the following formula (Fig. 2):

Indiv. ANB angle Panagiotidis and Witt
Skeletal class II was diagnosed if the measured ANB exceeded the individualised ANB by at least 1 °; skele-tal class III was determined if the measured ANB was at least 1 °smaller than the individualised ANB.A difference within a range of 1 °in either direction resulted in the diagnosis of a skeletal class I.
The CVMS method of Baccetti et al. [4] was used to define skeletal age: cervical vertebrae C2, C3 and C4 were evaluated in a single lateral cephalogram visually and classified into one of five CVMS (Table 1, Fig. 2).Not absolute sizes, but ratios of the distances in cervical vertebrae were important.In contrast to the original method, we distinguished between male and female patients.
Dental age was assessed using the method of Demirjian et al. [12]: orthopantomograms were analysed to distinguish eight mineralisation stages (A-H) of teeth 31 to 37 (Table 2, Fig. 3).All teeth were equally significant and every stage was associated with a certain Demirjian point value, ranging from 0 to 100.The summarised point values of all teeth yielded in an individualised dental age.These were compared to correlation tables that were separated by gender.Evaluating mineralisation stages, no absolute measurements were conducted, but ratios and visual inspection were solely performed.Crown height was defined as the greatest difference between cusp tip and cementoenamel junction (CEJ) and in case of different heights the mean was calculated.In borderline cases the previous stage was chosen.If no calcification was visible, the tooth was given stage 0, corresponding to a Demirjian point value of 0. When teeth were missing, a summarised point value was calculated without considering the missing tooth/teeth.
Statistical analysis was performed using IBM ® SPSS ® Statistics 21 (IBM Deutschland GmbH, Ehningen, Germany) and the online accessible NIWA (NIWA Taihoro Nukurangi, 2013) for calculating Lin's concordance correlation coefficient (CCC).Prior to the main investigation, 94 patients of all age groups were randomly selected for reliability testing.Blinding chronological age, lateral cephalograms and orthopantomograms were evaluated twice by the same rater (AD) with a time interval of at least 3 weeks and by a second rater to test intra-and interrater reliability, respectively.Lin's CCC was applied for scalar-metric variables (Demirjian point value), Cohen's kappa (κ) for categorical parameters (CVMS).The following interpretation, suggested by NIWA, was used: p > 0.99 perfect concordance, 0.95 < p ≤ 0.99 substantial, 0.90 ≤ p ≤ 0.95 moderate and p < 0.90 small concordance.
Normal distribution of the data was examined by Kolmogorov-Smirnov test and visual-optical inspection of histograms, variance homogeneity by Levene tests and visual-optical analysis of zpred vs. zresid plots.Analytical statistics for continuous normally distributed data included parametric independent two-tailed one-way analysis of variance (ANOVAs), corrected according to Welch in case of variance heterogeneity, with corresponding post

Results
From 1200 inspected patients, 760, who equally contributed to skeletal classes I, II and III in each chronological age group, were finally included (Fig. 1, Table 3).Gender was equally distributed among all patients (49.6% male, 50.4% female) and the four age groups, showing no significant difference between male and female patients (p > 0.05).
Inter-and intrarater reliability testing revealed at least substantial concordance for the determination of the Demirjian point value in the orthopantomograms and CVMS in the lateral cephalograms (κ > 0.8; ρc > 0.95).
Orthopantomograms      new regression formulae for the Central-European population in question (Table 6).
From 10 years onwards, all patients showed significantly different prevalences of CVMS in skeletal classes I, II and III: in the group 10-12 years (p = 0.006/p BH = 0.024), significantly more class III patients and significantly less class II patients showed CVMS I than children with class I. CVMS II occurred less frequently in class III than in classes II and I. CVMS III and IV were reached significantly more often by class II and significantly less by class III patients than in class I children.In the age group 12-14 years (p = 0.027/p BH = 0.054), CVMS III predominated and class II patients were the majority, followed by class I and finally class III patients.CVMS I was present mostly in class III patients, followed by class I and then class II children.CVMS II and IV showed similar prevalence in class I, II and III patients.In patients older than 14 years (p = 0.008/p BH = 0.032) CVMS IV was significantly dominated by skeletal class III patients, followed by class II and finally class I patients.The opposite was true for CVMS III, which was found most often in class I patients, followed by class II and III patients.However, the effect size of this statistically significant finding was only small (0.1 < Cramer's V < 0.3).In all chronological age groups, skeletal age according to Baccetti was significantly different from chronological age (p < 0.001/p BH < 0.001): whereas children aged at least 10 years were younger according to skeletal age, the opposite was true for the age group < 10 years.7).As the statistically significant effect of skeletal class was only small, we did not distinguish between skeletal classes I, II and III in these tables.Compared to skeletal ages determined originally by Baccetti et al. [4], our population showed higher skeletal ages for all CVMS (Table 7).According to the Wilcoxon rank sum test, chronological and skeletal age were not sig- nificantly different, when our tables were applied (p = 0.910; Table 6).

Discussion
The aim of this retrospective study was to analyse the skeletal and dental age of a contemporary Central-European growing population using the methods of Baccetti et al. [4] and Demirjian et al. [12], and to investigate the impact of gender and skeletal class.Furthermore, we established updated norm values for dental and skeletal age according to these methods.
The study population was homogenous regarding the distribution of skeletal classes I, II and III, and gender for all age groups except for the group > 14 years, which was dominated by male patients.This can be explained by the exclusion of patients with a Demirjian point value of 100 or CVMS V, which is reached earlier by girls.Overall homogeneity ensured that gender-and dysgnathia-related differences were not caused by selection bias, but by statistical effects.Due to ethical reasons and the as low as reasonably achievable (ALARA) principle for applying radiation doses, patients were not radiographically examined longitudinally to evaluate skeletal and dental development.Instead, a retrospective, cross-sectional study design and appropriate statistics were used to establish norm values, generalisable for the population of interest.
In this study, gender-related differences were found for dental and skeletal age.As in the original publication of Demirjian et al. [12], the Demirjian point value was significantly higher for girls than for boys in the same chronological age group, showing the earlier mineralisation of teeth in female patients.However, in the final dental age determined by Demirjian et al., no significant differences were found between boys and girls because the conversion tables considered this observation already.Concerning skeletal age, K girls revealed an accelerated development in almost all age groups as well.For example, between 10 and 12 years of age, CVMS I was present significantly more often in boys than in girls, whereas the opposite was true for CVMS III and IV.This phenomenon could be observed in the age groups 12-14 and > 14 years as well, illustrating that girls enter the pubertal growth spurt earlier and maintain this advancement until the end of growth.This difference was not evident for children younger than 10 years though, demonstrating that skeletal maturation does not start before the age of 10 years in both genders.Previous studies also found that girls start skeletal [7,22,31,50,59] and dental [12,16,27] maturation at a younger chronological age than boys.Since Baccetti et al. [4] did not analyse the influence of gender upon skeletal maturation, our results, incorporating this confounding factor, increase precision in the assessment of skeletal age.
According to our results, skeletal class seems to have a different influence on dental and skeletal age.In dental development, the only significant difference concerning skeletal class was observed in children below the age of 12 (Demirjian point value) or 10 years (dental age).Demirjian point values and dental age were higher in skeletal class II and lower in skeletal class III patients than in children presenting skeletal class I. Whereas overall our results indicate that dental age and Demirjian point value can be determined without differentiation of the skeletal classes, other authors reported an association between the sagittal jaw relation and dental development [32,57].Skeletal age, however, seems to be associated with the skeletal class.Whereas skeletal class II patients experienced growth spurt first, followed by class I patients, children with skeletal class III showed maximum growth latest, i.e. at the age of 14 years or older.Although this difference was statistically significant, the effect size was only small (0.1 < Cramer's V < 0.3).Therefore, our results indicate that no additional effort in daily practice seems to be necessary to determine skeletal age considering the skeletal class.Further investigations are required to analyse the definite impact of sagittal dysgnathia on skeletal maturity.
Comparing dental and skeletal age of our study with the original publications of Demirjian et al. [12] and Baccetti et al. [4], respectively, differences to the original norm values are obvious.In most cases (< 14 years) of our recent population, dental age determined by Demirjian's tables was significantly higher than the chronological age and would therefore result in an overestimation of patient age.A possible explanation for this difference might be the accelerated dental maturation of our contemporary Central-European population compared to Demirjian's Canadian population from the 1970s.Such ethnically and population-based variances, when applying Demirjian's method for dental age assessment were also described by other au-thors [1,2,8,16,27,28,34,36,56]. Therefore, we established updated norm values for dental age based on the mineralisation method of Demirjian et al. for a contemporary Central-European adolescent population using regression analysis as a growth function.Whereas skeletal class did not significantly affect dental age and therefore was neglected, gender was associated with dental development, leading to separate equations for boys and girls.The deviation from a norm value to be accepted was 2 years [43].Applying the new formulae, reliability testing revealed no significant difference between mean chronological age and mean dental age, proving the validity of our equations.
Comparing mean chronological age and mean skeletal age as determined by Baccetti's CVMS method, the latter was significantly higher.This shows that our investigated German patients had a delayed skeletal maturity compared to Baccetti's Italian population, and that Central-European children seem to reach their pubertal growth spurt later than South-European adolescents.Therefore, new tables for skeletal age were established based on the Central-European population evaluated in this study to generate updated norm values without significant difference from the mean chronological age.Because of significant gender-related differences, the tables were separated for boys and girls.Since the clinical effect of skeletal class on skeletal age was small and to simplify the use of the tables in routine orthodontic diagnostics, subdivision for skeletal class I, II and III was not included in the new tables.Other authors, in contrast, did report an impact of skeletal class on skeletal age.Skeletal class III patients showed a longer pubertal growth peak starting at the same timepoint compared to skeletal class I children [25,30].
Our findings are clinically relevant in several points.First, skeletal maturity assessed via cervical vertebrae maturation is significantly associated with mandibular growth potential [11,14,38,44] and therefore influences treatment planning, when mandibular deviations or deficiencies are evident.Also orthodontic interventions in the upper jaw are affected by the remaining growth potential, as rapid maxillary expansion (RME) and combined RME/ protraction are more effective before pubertal growth peak and at earlier stages of dental development, respectively [3,15].This could be explained by an association between skeletal maturity, assessed via cervical vertebrae maturation, and midpalatal suture maturity [35].Dental age assessment is also clinically relevant for analysing facial growth, even if it does not allow identification of timing of the pubertal growth spurt because the latter is more associated with the development of cervical vertebrae [7,39,41,50] and craniofacial growth [57].Furthermore, dental age assessment might be easier to conduct, especially for general dentists having no lateral cephalogram devices, hence allowing a first examination.According to R 2 , the formulae explain 54.6% and 57.6% of variance in dental age of girls and boys, respectively.The remaining 45.4% and 42.4% of variance occur due to other reasons, including genetics, skeletal class, malnutrition and obesity [21,42,47].Hence, even though dental and skeletal age assessment by the methods described reveal reliability, they should not be used solely, but as an addition to other clinical investigations including body height, onset of the menarche and signs and symptoms of disorders, each affecting physiological development.Finally, the choice of treatment devices depends on biological age: whereas skeletal maturity influences the success of functional orthopaedic appliances, dental development determines the time for insertion of fixed appliances.
A limitation of the applied method is radiographic imaging that might be imprecise due to anatomical, technical and rater-based reasons.To ensure a certain level of quality, radiographs of minor quality were excluded from the study.Furthermore, since radiographs from different treatment stages were included, some patients had already experienced functional orthopaedic therapies, which might have affected growth.As no longitudinal radiographic examination of a single individual patient was performed, actual craniofacial growth might have changed the skeletal class and hence affected the statistical analysis of the association between skeletal class and dental/skeletal age.However, our method to determine skeletal class, i.e. using the individualised ANB angle by Panagiotidis and Witt, considered already sagittal and vertical parameters, thereby increasing diagnostic precision.Another limiting factor is that the Demirjian method has no rule how to proceed in case of missing permanent teeth in the third quadrant.Although Demirjian point values appear to be precise, even if some teeth in the left quadrant are missing [33], it should be considered that tooth agenesis might influence rate of development of the neighbouring teeth [5].Furthermore, premature loss of deciduous teeth was not taken into account.These might have eventually accelerated the eruption of permanent teeth, but not the rate of root formation [13].Next, the CVMS method relies on the subjective analysis of an orthodontist.This could have introduced mistakes in the analysis of skeletal age.Also, Demirjian's method is affected by some degree of subjective evaluation.However, interand intrarater reliability showed at least substantial measurement concordance and thus, guaranteed reliable measurements.Although the CVM stages allow a classification of remaining growth potential, the actual velocity of the peak mandibular growth cannot be predicted [6], introducing some uncertainty in treatment planning.Another limitation of this study is that the inclusion criteria did not ask for a certain, e.g.Caucasian, ethnicity.Thus, this fact was not evaluated as a confounder in the present study, although the German location of study places has probably ensured a mainly Caucasian population.The impact of ethnicity is controversially discussed in literature.Whereas some authors did not find a significant population-and ethnicityrelated difference for skeletal and dental age assessment, others did [10,26,49].Finally, according to the inclusion criteria, only patients without any systemic diseases were considered, so that our results can be generalised with certainty only to generally healthy patients.

Conclusions
Using a regression formula based on the mineralisation stages of the left mandibular teeth and conversion tables for CVM stages, we established new norm values to assess dental and skeletal age according to the methods by Demirjian et al. and Baccetti et al., respectively.The new values, updated for a contemporary Central-European growing population and separated by gender, might improve orthodontic diagnosis and be helpful in planning orthodontic treatment.Further investigations should be conducted to test the clinical relevance of the association between the skeletal class and skeletal age.

Fig. 2
Fig. 2 Cephalometric analysis to analyse skeletal class according to the individualised ANB angle by Panagiotidis and Witt [45] and maturation stages of cervical vertebrae C2-C4.N nasion, S sella, A point A, B point B, Me menton, Go gonion, ML mandibular line, NSL nasion-sella line, angle 1 ML-NSL inclination of the mandible, angle 2 SNA maxillary prognathism, angle 3 ANB sagittal relation between upper and lower jaw (skeletal class) Abb. 2 FRS(Fernröntgenseitenbild)-Analyse zur Bestimmung der skelettalen Klasse anhand des individualisierten ANB-Winkels von Panagiotidis und Witt [45] sowie der Reifestadien der Halswirbel C2-C4.N Nasion, S Sella, A A-Punkt, B B-Punkt, Me Menton, Go Gonion, ML Mandibularlinie, NSL Nasion-Sella-Linie, Winkel 1 ML-NSL Inklination des Unterkiefers, Winkel 2 SNA maxillärer Prognathiegrad, Winkel 3 ANB sagittale Relation zwischen Ober-und Unterkiefer (skelettale Klasse) Cervical vertebrae maturation stages (CVMS) I-V to assess skeletal age with lateral cephalograms.(Technique modified after Baccetti et al. [4]) Tab. 1 CVMS I-V ("Reifestadien der Halswirbel") zur Ermittlung des skelettalen Alters anhand von FRS(Fernröntgenseitenbild)-Aufnahmen.(Methodik modifiziert nach Baccetti et al. [4]) CVMS Figure Definition Mandibular growth potential CVMS I Lower borders of C2-C4 are flat.C2 may have a concavity.C3 and C4 are trapezoidal and conical from posterior to anterior Growth peak occurs at least one year after this stage CVMS II Lower borders of C2 and C3 are concave.C3 and C4 are trapezoidal or horizontally rectangular Growth peak occurs within half a year after this stage CVMS III Lower borders of C2, C3 and C4 are concave.C3 and C4 are horizontally rectangular Growth peak has started one or two years before this stage CVMS IV Lower borders of C2, C3 and C4 are concave.At least one of C3 and C4 is square, otherwise the other is horizontally rectangular Growth peak has started within one year before this stage CVMS V Lower borders of C2, C3 and C4 are concave.At least one of C3 and C4 is vertically rectangular, otherwise the other is square Growth peak has started within two years before this stage hoc tests.If continuous data were not normally distributed, nonparametrical two-tailed independent Mann-Whitney or Kruskal-Wallis tests were performed.Categorical parameters were analysed with exact Fisher tests.Dependent twotailed paired t-tests and nonparametrical, dependent twotailed Wilcoxon rank sum tests were applied to assess reliability of the analysis of dental age (Demirjian, Regensburger analysis) and skeletal age (Baccetti, Regensburger analysis)respectively.To establish a formula for dental age assessment, nonlinear regression analysis with a growth function (e-function) was applied, showing best fit according to the coefficient of determination R 2 .The analysis of skeletal age was based on conversion tables for CVMS and corresponding chronological ages, incorporating the results of descriptive and analytical statistics.Significance level was set at p ≤ 0.05, with corrections for multiple testing performed according to Bonferroni-Holm (BH).To judge clinical relevance of significant differences, Pearson's correlation coefficient r and Cramer's V were calculated as effect sizes for metrical and categorical variables, respectively; r or V > 0.5 represents a large, r or V > 0.3 a medium and r or V > 0.1 a small effect.

Table 4 .
Demirjian point values of girls were significantly higher of 551 patients were included in dental age assessment.Their distribution regarding age groups, mean values of chronological age, Demirjian point value and dental age after Demirjian is presented in

Table 2
Eight mineralisation stages

Table 3
Distribution of skeletal classes (I, II, III) within the total study collective and the four age groups Tab. 3 Verteilung der skelettalen Klassen (I, II, III) innerhalb des gesamten Studienkollektivs und der 4 Altersgruppen

Table 4
Distribution of orthopantomograms (n), chronological age (in years), Demirjian point value and dental age according to Demirjian (in years) within the chronological age groups and the total study populationTable 5)shows that with increasing chronological age, CVMS, i.e. skeletal age, increased as well.Except for the group ≤ 10 years (p = 0.548), the CVMS were significantly different for boys and girls (p < 0.001/p BH < 0.001).
n absolute frequency of orthopantomograms, M mean value, SD standard deviation age groups (

Table 6
6eliability of the updated norm values for dental and skeletal age assessment Tab.6Reliabilität der aktualisierten Normwerte für die dentale und die skelettale Altersbestimmung n number orthopantomograms (dental)/lateral cephalograms (skeletal), M mean age in years, SD standard deviation, t/W test statistics paired twotailed t-/Wilcoxon rank sum test, Df degrees of freedom, z standard test statistics, p significance value, r effect size (Pearson correlation coefficient), 95% HL CI 95% Hodges-Lehmann confidence interval