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Glycomacropeptide administration attenuates airway inflammation and remodeling associated to allergic asthma in rat

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Abstract

Objective

Glycomacropeptide (GMP) is a bioactive peptide derived from milk that has been reported to exhibit a range of anti-inflammatory and immunomodulatory properties. The aim of this study was to analyze the prophylactic effect of GMP administration on airway inflammation and remodeling in an experimental model of asthmatic rat.

Methods

Animals treated orally with or without GMP (500 mg/kg/day) were ovalbumin-sensitized and -nebulized and several indicators of Th2 response, airway structural changes and inflammatory cells recruitment were evaluated.

Results

Treatment with GMP prior and during asthma development resulted in reduction of allergen-specific IgE titers in serum and blood eosinophilia. Also, GMP substantially suppressed the recruitment of inflammatory cells to bronchoalveolar compartment. Histological studies demonstrated that GMP markedly inhibits eosinophils infiltration, goblet cells hyperplasia and collagen deposit in lung tissue. The latter effect was related with an inhibition in transforming growth factor-β expression. In addition, expression of interleukin-5 and -13 were substantially inhibited in lung while that of interleukin-10 was increased.

Conclusion

Our results suggest that administration of GMP may prevent the development of an excessive Th2 response in asthma and effectively ameliorates the progression of the disease.

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Acknowledgments

This work was supported by grants PIBB 14-1 and 240921 from the Autonomous University of Aguascalientes and CONACYT, respectively. Renata Roldán has a master fellowship from CONACYT. The authors wish to thanks Istvan Berczi for reviewing the paper, Berenice Barrón for excellent technical assistance and MVZ José Luis Ponce for providing the animals for the study.

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Authors and Affiliations

  1. Department of Microbiology, Basic Science Center, Autonomous University of Aguascalientes, Av. Universidad # 940, 20131, Aguascalientes, AGS, Mexico

    Nuria Renata Roldán, Mariela Jiménez, Daniel Cervantes-García, Estefanía Marín & Eva Salinas

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  1. Nuria Renata Roldán
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  2. Mariela Jiménez
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  3. Daniel Cervantes-García
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  4. Estefanía Marín
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Correspondence to Eva Salinas.

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Roldán, N.R., Jiménez, M., Cervantes-García, D. et al. Glycomacropeptide administration attenuates airway inflammation and remodeling associated to allergic asthma in rat. Inflamm. Res. 65, 273–283 (2016). https://doi.org/10.1007/s00011-015-0913-y

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  • DOI: https://doi.org/10.1007/s00011-015-0913-y

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