Abstract
Living zygotes of tobacco (Nicotiana Tabacum L.) SR-1 were isolated and culturedin vitro by the microculture technique. Fertile plants were regenerated from the calli derived from cultured zygotes via organogenesis. Ovules were collected 120 h after pollination and used as feeder. MS combined with KM8p was selected as basic medium in the experiment. Zygotes isolated from ovules 108 h after pollination turned out to be suitable material forin vitro culture. Over 80% such zygotes could divide and around 10% of them could grow into calli and regenerate fertile plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Leduc, N., Matthys-Rochon, E., Rougier, M. et al., Isolated maize zygotes mimicin vivo embryonic development and express microinjected genes when culturedin vitro, Develpomental Biology, 1996, 177: 190–203.
Holm, P. B., Olsen, O., Schnorf, M. et al., Transformation of barley by microinjection into isolated zygote protoplast, Transgenic Research, 2000, 9: 21–32.
Li, S. T., Wang, S. H., Yang, H. Y., Transformation of plant young proembryo by electroporation, Chinese Science Bulletin, 2000, 45(13): 1202–1206.
Li, S. T., Yang, H. Y., Gene transfer into isolated and cultured tobacco zygotes by a specially designed device for electroporation, Plant Cell Reports, 2000, 19: 1184–1187.
Kranz, E., Lörz, H.,In vitro fertilization with isolated, single gametes results in zygotic embryogenesis and fertile maize plants, Plant Cell, 1993, 5: 739–746.
Mól, R., Matthys-Rochon, E., Dumas, C.,In vitro culture of fertilized embryo sacs of maize: zygotes and two-celled proembryos can develop into plants, Planta, 1993, 189: 213–217.
Mól, R., Matthys-Rochon, E., Dumas, C., Embryogenesis and plant regeneration from maize zygotes byin vitro culture of fertilized embryo sac, Plant Cell Reports, 1995, 14: 743–747.
Holm, P. B., Knudsen, S., Mouritzen, P. et al., Regeneration of fertile barley plants from mechanically isolated protoplasts of the fertilized egg cell, Plant Cell, 1994, 6: 531–543.
Kumlehn, J., Lörz, H., Kranz, E., Differentiation of isolated wheat zygotes into embryos and normal plants, Planta, 1998, 205: 327–333.
Zhang, J., Dong, W. H., Galli, A. et al., Regeneration of fertile plants from isolated zygotes of rice (Oryza sativa), Plant Cell Reports, 1999, 19: 128–132.
Fu, C. M., Sun, M. X., Zhou, C. et al., Isolation of fertilized embryo sacs and zygotes and triggering of zygotes divisionin vitro inNicotiana Tabacum L., Acta Botanica Sinica, 1996, 38: 262–267.
Li, S. T., Fu, Y., Sun, M. X. et al.,In vitro development of zygotes and two-cell proembryo inNicotiana, Acta Biologiae Experimental-4 Sinica, 1998, 31: 309–315.
Kao, K. N., Michayluk, M. R., Nutrient requirements for growth ofVicia hajastana cells and protoplasts at a very low population density in liquid media, Planta, 1975, 126: 105–110.
Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue culture, Plant Physiol., 1962, 15: 473–497.
Gambog, O. L., Miller, R. A., Ojima, K., Nutrient requirement of suspension cultures of soybean root cells, Experimental Cell Research, 1968, 50: 151–158.
Leduc, N., Matthys-Rochon, E., Dumas, C., Deleterious effect of minimal enzymatic treatments on the development of isolated maize embryo sacs in culture, Sex Plant Reproduction, 1995, 8: 313–317.
Zhao, J., Zhou, C., Yang, H. Y., Isolation andin vitro culture of zygotes and central cells ofOryza sativa L., Plant Cell Reports, 2000, 19: 321–326.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
He, Y., Sun, M. & Yang, H. Regeneration of fertile plants from isolated tobacco zygotes byin vitro culture. Chin. Sci. Bull. 49, 810–814 (2004). https://doi.org/10.1007/BF02889752
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02889752