Skip to main content
SpringerLink
Log in

Sterol pathway in yeast. Identification and properties of mutant strains defective in mevalonate diphosphate decarboxylase and farnesyl diphosphate synthetase

  • Plant and Fungal Sterols: Biosynthesis, Metabolism and Function Papers Presented at a Symposium Held at the AOCS Annual Meeting. Baltimore, MD, April 1990
  • Article
  • Published:
Lipids

Abstract

Yeast mutant strains auxotrophic for ergosterol and blocked in mevalonate diphosphate decarboxylase (erg19) and farnesyl diphosphate (FPP) synthetase (erg20) were isolated. The main feature of the mutants blocked in FPP synthetase is their ability to excrete prenyl alcohols, such as geraniol and farnesol. The isolation of the functionalERG20 gene allowed us to show that farnesyl diphosphate synthetase could be a rate limiting enzyme in ergosterol biosynthesis in yeast.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DMAPP:

dimethylallyl diphosphate

DTT:

dithiothreitol

ERG:

functional gene in ergosterol pathway

erg:

gene defective in ergosterol pathway

FPP:

farnesyl diphosphate

GC/MS:

gas chromatography/mass spectrometry

GPP:

geranyl diphosphate

HMG-CoA:

hydroxymethylglutaryl-Coenzyme A

IPP:

isopentenyl diphosphate

MVA:

mevalonic acid

MVAP:

mevalonate phosphate

MVAPP:

mevalonate diphosphate

TLC:

thin-layer chromatography

[]:

denotes plasmid-carrier state

References

  1. Woods, R. (1971)J. Bacteriol. 108, 69–73.

    PubMed  CAS  Google Scholar 

  2. Resnick, M.A., and Mortimer, M.K. (1966)J. Bacteriol. 92, 597–602.

    PubMed  CAS  Google Scholar 

  3. Karst, F., and Lacroute, F. (1973)Biochem. Biophys. Res. Commun. 52, 741–747.

    Article  PubMed  CAS  Google Scholar 

  4. M'Baya, B., Fegueur, M., Servouse, M., and Karst, F. (1989)Lipids 24, 1020–1023.

    PubMed  Google Scholar 

  5. Chambon, C., Ladeveze, V., Oulmouden, A., Servouse, M., and Karst, F. (1990)Curr. Genet. 18, 41–46.

    Article  PubMed  CAS  Google Scholar 

  6. Torriani, A. (1960)Biochim. Biophys. Acta 38, 460–479.

    Article  PubMed  CAS  Google Scholar 

  7. Karst, F., and Lacroute, F. (1977)Mol. Gen. Genet. 154, 269–277.

    Article  PubMed  CAS  Google Scholar 

  8. Basson, M.E., Thorness, M., and Rine, J. (1986)Proc. Natl. Acad. Sci. USA 83, 5563–5567.

    Article  PubMed  CAS  Google Scholar 

  9. Oulmouden, A., and Karst, F. (1990)Gene 88, 253–257.

    Article  PubMed  CAS  Google Scholar 

  10. Servouse, M., and Karst, F. (1986)Biochem. J. 240, 541–547.

    PubMed  CAS  Google Scholar 

  11. Servouse, M., Mons, N., Baillargeat, J.L., and Karst, F. (1984)Biochem. Biophys. Res. Commun. 123, 424–430.

    Article  PubMed  CAS  Google Scholar 

  12. Anderson, M.S., Yarger, J.G., Burk, C.L., and Poulter, C.D., (1989)J. Biol. Chem. 264, 19176–19184.

    PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Francis Karst

    Present address: Laboratoire de Biochimie et Génétique des Microoorganismes Université de Poitiers, 40, avenue du Recleur Pineau, F-86022, Poitiers Cedex, France

Authors and Affiliations

  1. Laboratoire de Microbiologie, Centre de Recherches Pernod-Ricard, F-94015, Créteil, France

    Catherine Javelof & Barbu Vladescu

  2. Laboratoire de Biochimie et Génétique des Microoorganismes Université de Poitiers, 40, avenue du Recleur Pineau, F-86022, Poitiers Cedex, France

    Christophe Chambon, Veronique Ladeveze, Madeleine Servouse & Laurence Blanchard

Authors
  1. Christophe Chambon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Veronique Ladeveze
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Madeleine Servouse
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Laurence Blanchard
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Catherine Javelof
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Barbu Vladescu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Francis Karst
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Chambon, C., Ladeveze, V., Servouse, M. et al. Sterol pathway in yeast. Identification and properties of mutant strains defective in mevalonate diphosphate decarboxylase and farnesyl diphosphate synthetase. Lipids 26, 633–636 (1991). https://doi.org/10.1007/BF02536428

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02536428

Keywords

Search

Navigation