Skip to main content
SpringerLink
Log in

Beneficial effect of octreotide treatment in acute pancreatitis in rats

  • Original Articles
  • Published:
International journal of pancreatology Aims and scope Submit manuscript

Summary

Conclusions

Octreotide treatment contributes to the regulation of tumor necrosis factor (TNF) production in sodium taurocholate-induced acute necrotizing pancreatitis in rats. Owing to its complex effect, octreotide can partially ameliorate the deleterious consequences of acute necrotizing pancreatitis. Elevated TNF and interleukin-6 (IL-6) levels in the peritoneal fluid may be considered a consequence of the activation of peritoneal macrophages.

Background

The effects of octreotide on exocrine pancreatic function have been investigated in numerous studies, but little attention has been paid to its influence on cytokine production in acute pancreatitis.

Methods

Acute pancreatitis was induced by the retrograde injection of taurocholic acid into the pancreatic duct in male Wistar rats. Serum amylase activity, wet pancreatic weight/body weight (pw/bw) ratio, and TNF and IL-6 levels were measured. Four μg/kg of octreotide was administered subcutaneously at the time of induction of pancreatitis and 24 or 48 h later. Rats were sacrificed 6, 24, 48, or 72 h after the operation.

Results

The serum amylase level and pancreatic weight to body weight ratio were decreased significantly in the octreotide-treated group. The serum TNF level was decreased significantly in the octreotide-treated group as compared with the control group at 6, 24, and 48 h (0.6±1.5, 2.0±3.3, and 0 vs. 50±15.5, 37.5 ±18.4, and 13.1±12.5 U/mL, respectively). The ascites TNF level was decreased to 0 in the octreotide-treated group and was elevated in the control group at 72 h (28.0±49.0 U/mL). IL-6 production in ascites was extremely high in both groups at 6 h (80,000±43, 817 pg/mL and 58, 500±33 335 pg/mL), but the difference was not significant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Grosman I, Simon D. Potential gastrointestinal uses of somatostatin and its synthetic analogue octreotide.Am J Gastroenter 1990; 85(9): 1061–1072.

    PubMed  CAS  Google Scholar 

  2. Schwedes U, Althoff PH, Klempa J, et al. Effects of somatostatin on bile induced acute haemorrhagic pancreatitis in the dog.Horm Metab Res 1979; 11: 647.

    Google Scholar 

  3. Baxter JN, Jenkins SA, Cowell DC, et al. Effects of somatostatin and long acting somatostatin analogue on the prevention and treatment of experimentally induced acute pancreatitis in the rat.Br J Surg 1985; 72: 382–385.

    Article  PubMed  CAS  Google Scholar 

  4. Zhu ZH, Holt S, El-Lbishi MS et al. A somatostatin analogue is protective against retrograde bile salt-induced pancreatitis in the rat.Pancreas 1991; 6: 609–613.

    Article  PubMed  CAS  Google Scholar 

  5. McKay C, Baxter J, Imrie C. A randomized, controlled trial of octreotide in the management of patients with acute pancreatitis.Int J Pancreatol 1997; 21: 13–19.

    PubMed  CAS  Google Scholar 

  6. Luengo L, Vicente V, Gris F, et al. Influence of somatostatin in the evolution of acute pancreatitis: a prospective randomized study.Int J Pancreatol 1994; 15: 139–144.

    PubMed  CAS  Google Scholar 

  7. Buchler MW, Binder M, Friess H. Role of somatostatin and its analogues in the treatment of acute and chronic pancreatitis.Gut 1994; 35(Suppl): S15-S19.

    Article  PubMed  CAS  Google Scholar 

  8. Friess H, Klempa I, Hermanek P, et al. Prophylaxis of complications after pancreatic surgery: results of a multicenter trial in Germany.Digestion 1994; 55(Suppl 1): 35–40.

    Google Scholar 

  9. Farkas G, Leindler L, Szederkenyi E. Beneficial effect of Sandostatin, a long-acting somatostatin analog, in pancreatic surgery.Hepatogastroenterology 1993; 40: 182,183.

    Google Scholar 

  10. Bordas JM, Toledo V, Mondelo R, et al. Prevention of pancreatic reactions by bolus somatostatin administration in patients undergoing endoscopic retrograde cholangiopancreatography and endoscopic sphincterotomy.Horm Res 1988; 29: 106–108.

    Article  PubMed  CAS  Google Scholar 

  11. van Ooijen B, Tinga CJ, Kort WJ, et al. Effects of longacting somatostatin analog (SMS 201-995) on eicosanoid synthesis and survival in rats with acute necrotizing pancreatitis.Dig Dis Sci 1992; 37: 1434–1440.

    Article  PubMed  Google Scholar 

  12. Baxter JN, Jenkins SA, Day DW, et al. Effects of somatostatin analogue (SMS 201-995) on hepatic and splenic reticulo-endothelial function in the rat.Br J Surg 1985; 72: 1005–1008.

    Article  PubMed  CAS  Google Scholar 

  13. Todd KE, Lewis MPN, Gloor B, et al. ETa/Etb endothelin antagonist ameliorates systemic inflammation in murine model of acute haemorrhagic pancreatitis.Br J Surg 1997; 84: 1585.

    Google Scholar 

  14. Peluso G, Petillo O, Melone MA, et al. Modulation of cytokine production in activated human monocytes by somatostatin.Neuropeptides 1996; 30: 443–451.

    Article  PubMed  CAS  Google Scholar 

  15. Karalis K, Mastorakos G, Chrousos GP, et al. Somatostatin analogues suppress the inflammatory reaction in vivo.J Clin Invest 1994; 93: 2000–2006.

    Article  PubMed  CAS  Google Scholar 

  16. Balibrea JL, Arias-Diaz J, Garcia C, et al. Effect of pentoxifylline and somatostatin on tumor necrosis factor production by human pulmonary macrophages.Circ Shock 1994; 43: 51–56.

    PubMed  CAS  Google Scholar 

  17. Badia JM, Whawell SA, Scott-Coombs DM, et al. Peritoneal and systemic cytokine response to laparotomy.Br J Surg 1996; 83: 347,348.

    Article  PubMed  CAS  Google Scholar 

  18. Aho J, Koskensalo SM-L, Nevalainen TJ. Experimental pancreatitis in the rat: sodium taurocholate-induced acute haemorrhagic pancreatitis.,Scand J Gastroenterol 1980; 15: 411–416.

    Article  PubMed  CAS  Google Scholar 

  19. Ceska M, Birath K, Brown B. A new and rapid method for the clinical determination of amylase activities in human serum and urine.Clin Chem Acta 1969; 26: 437–444.

    Article  CAS  Google Scholar 

  20. Espevik T, Niessen-Meyer JA. A highly sensitive cell line WEHI-164 for measuring cytotoxic factor/tumor necrosis factor.J Immunol Methods 1986; 95: 99–105.

    Article  PubMed  CAS  Google Scholar 

  21. Arden LA, deGroot ER, Shaap OL. Production of hybridoma growth factor by human monocytes.Eur J Immunology 1987; 17: 1411–1416.

    Article  Google Scholar 

  22. Hughes CB, Grewal HP, Gaber LW. Anti-TNFα therapy improves survival and ameliorates the pathophysiologic sequelas in acute pancreatitis in the rat.Am J Surg 1996; 171: 274–280.

    Article  PubMed  CAS  Google Scholar 

  23. Kusterer K, Enghofer M, Poschmann T, et al. The effect of somatostatin, gabexate mesilate and dextran 40 on the microcirculation in sodium taurocholate-induced pancreatitis.Acta Physiol Hung 1992; 80: 407–415.

    PubMed  CAS  Google Scholar 

  24. Steinberg WM, Schlesselman SE. Treatment of acute pancreatitis: comparison of animal and human studies.Gastroenterology 1987; 93: 1420–1427.

    PubMed  CAS  Google Scholar 

  25. Hoffmann TF, Uhl E, Messmer K. Protective effect of the somatostatin analogue octreotide in ischemia/reperfusion induced acute pancreatitis in rats.Pancreas 1996; 12: 286–293.

    Article  PubMed  CAS  Google Scholar 

  26. Paran H, Klausner J, Siegel A, et al. Effect of the somatostatin analogue octreotide on experimental pancreatitis in rats.J Surg Res 1996; 62: 201–206.

    Article  PubMed  CAS  Google Scholar 

  27. Kaplan O, Kaplan D, Casif E, et al. Effects of delayed administration of octreotide in acute experimental pancreatitis.J Surg Res 1996; 62: 109–117.

    Article  PubMed  CAS  Google Scholar 

  28. Tulassay Z, Kisfalvi K, Papp M. The effect of the long-acting somatostatin analogue octreotide on cerulein-induced pancreatic injuries in rats.Z Gastroenterol 1995; 33: 99–102.

    PubMed  CAS  Google Scholar 

  29. De Rai P, Franciosi C, Confalonier GM, et al. Effects of somatostatin on acute pancreatitis induced in rats by injection of taurocholate and trypsin into a temporarily closed duodenal loop.Int J Pancreatol 1988; 3: 367–374.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Albert Szent-Gyorgyi Medical University, Szeged, Hungary

    Zsuzsanna Nagy

  2. First Department of Medicine, Albert Szent-Gyorgyi Medical University, Szeged, Hungary

    Katalin Jarmay, Tamas Takacs & Janos Lonovics

  3. Department of Surgery, Albert Szent-Gyorgyi Medical University, Pécsiu 4, 6720, Szegedi, Hungary

    Janos Marton, Zsuzsanna Szasz, Adam Balogh & Gyula Farkas

Authors
  1. Janos Marton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zsuzsanna Szasz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zsuzsanna Nagy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Katalin Jarmay
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tamas Takacs
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Janos Lonovics
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Adam Balogh
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Gyula Farkas
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Marton, J., Szasz, Z., Nagy, Z. et al. Beneficial effect of octreotide treatment in acute pancreatitis in rats. Int J Pancreatol 24, 203–210 (1998). https://doi.org/10.1007/BF02788423

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02788423

Key Words

Search

Navigation