Abstract
The production of L-asparaginase by two mutants ofSerratia marcescens grown on 14 different media was studied. The enzyme content increased from trace levels to 2.4 international units per ml when the organisms were grown in glycerol-peptone yeast extract medium. Glucose was the best carbon source under aerobic conditions. The enzyme content increased when L-asparagine was present in the growth medium.
Similar content being viewed by others
References
Bilimoria, M. H. (1969)Appl. Microbiol.,18, 1025.
Boeck, L. D., Squires, R. W., Wilson, M. W. and Ho, P. P. K. (1970)Appl. Microbiol.,20, 964.
Boeck, L. D. and Ho, P. P. K. (1973)Can. J. Microbiol.,19, 1251.
Cedar, H. H. and Schwartz, J. H. (1968)J. Bacteriol.,96, 2043.
Heinemann, B. and Howard, A. J. (1969)Appl. Microbiol.,18, 550.
Heinemann, B., Howard, A. J. and Palocz, H. (1970)Appl. Microbiol.,19, 800.
Jeffries, J. C. (1976) inSecond international symposium on genetics of industrial microbes, ed. K. P. Macdonald (London: Academic Press), p. 301.
Khan, A. A., Pal, S. P., Raghavan, S. R. V. and Bhattacharyya, P. K. (1970)Biochem. Biophys., Res. Common.,41, 425.
Liu, F. S. and Zajic, J. E. (1972)Appl. Microbiol.,33, 667.
Meister, A. (1955) inMethods in Enzymol,2, 283.
Netrval, J. and Smekal, F. (1970)Biochemical session of Czechoslovak Biochem. Scociety Abstr., p. 101.
Netrval, J. (1973)Arch. Microbiol.,92, 345.
Netrval, J. (1977)Folia Microbiol.,22, 106.
Nikolayev, A. Ya., Eyseev, L. P., Tyulpanova, E. S. and Abdumalikova, A. Kh. (1969)Biokhimiya,34, 352.
Nikolayev, A. Ya., Sololov, N. N. and Mardeshev, S. R. (1971)Biokhimiya,36,643.
Ozolins, R., Grivina, P., Gvozdyak, R. I., Garkavaye, L. F. and Kraizman, Z. V. (1973) cited inChem Abstr 806 (No. 11) 5838 2ULaty PSR Zinat Akad Vestes, 7,81.
Pastuszak, I. and Szymona, M. (1976)Acta Biochem. Polon.,23,37.
Peter, P. K. H., Elizabath, B., Milikin, J. L. B., Edward, L. B., Phillip, J. B., Bruce, H. F., La Verne, D. B. and Robert, W. S. (1970)J. Biol. Chem.,245, 3708
Roberts, J., Burson, G. and Hill, J. M. (1968)J. Bacteriol.,95, 2117.
Singh, D. V., Sukumaran, C. P. and Mahadevan, P. R. (1977)Indian J. Exp. Biol.,15, 156.
Smirrous, I. P., Zanin, V. A. and Berezov, T. T. (1976)Prikl. Biokhim. Microbiol.,12, 14.
Tchan, V. T., Asano, R. and Kabayashi, M. (1971)J. Ferment. Technol.,49, 733.
Tyulpanova, E. S., Eremenko, V. V. and Mardashev, S. R. (1972)Microbiologiya,41, 423.
Wriston, J. C. Jr. and Yellin, T. (1973) inAdvances in enzymol, ed. Alton Meister (New York: Interscience and John Wiley), p. 185.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sukumaran, C.P., Singh, D.V. & Mahadevan, P.R. Synthesis of L-asparaginase bySerratia marcescens (Nima). J Biosci 1, 263–269 (1979). https://doi.org/10.1007/BF02716875
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02716875