Abstract
A highly accurate and precise procedure deserving the name of “definitive method” has been devised for the determination of copper in biological materials. The method is based on combination of neutron activation and very selective and quantitative post-irradiation separation of copper from other radionuclides by extraction chromatography, using columns with LIX 70 on Bio-Beads SM-1, followed by gamma-ray spectrometric measurement. All potential sources of errors were carefully examined and eliminated or appropriate corrections were introduced into the procedure. The method contains several warning mechanisms safeguarding against making gross errors. Limit of detection for rather short irradiation time (tir≤1 h) (which enables radiochemical work without sophisticated shielding) amounts to 15 μg/kg. Results for copper concentration in several NBS, IAEA and other certified reference materials are presented and a new “recommended value” for IAEA's Milk Powder (A-11) is proposed.
Similar content being viewed by others
References
R. DYBCZYŃSKI, A. VEGLIA, O. SUSCHNY, Trace Element Analytical Chemistry in Medicine and Biology, P. BRÄTTER, P. SCHRAMEL [Eds], W. de Gruyter, New York, 1980, p. 657.
R. M. PARR, Report No. 2, Intercomparison of Minor and Trace Elements in IAEA Animal Muscle [H-4], IAEA/RL/69, IAEA, Vienna, October 1980.
L. PSZONICKI, A. VEGLIA, R. DYBCZYŃSKI, O. SUSCHNY, Report on Intercomparison V-8 of the Determination of Trace Elements in Rye Flour, IAEA/RL/91, IAEA, Vienna, January 1982.
G. H. MORRISON, Anal. Chem., 43 (1971) 22A.
D. J. Von LEHMDEN, R. H. JNGERS, R. E. LEE, Jr., Anal. Chem., 46 (1974) 239.
R. DYBCZYŃSKI, in: Quality Assurance in Biomedical Neutron Activation Analysis, IAEA-TECDOC-323, IAEA, Vienna, 1984, p. 39.
J. VERSIECK, R. CORNELIS, Anal. Chim. Acta, 116 (1980) 217.
R. DYBCZYŃSKI, in: Nuclear Techniques for Analysis of Environmental Samples, International Atomic Energy Agency Report, IAEA/RL/135, IAEA, Vienna, Dec. 1985.
G. A. URIANO, C. C. GRAVATT, Crit. Rev. Anal. Chem., 6 (1997) 361.
K. HEYDORN, Neutron Activation Analysis for Clinical Trace Element Research, Vol. II, CRC Press Inc., Boca Raton, 1984, p. 3.
W. R. WOLF, Anal. Chem., 50 (1978) 1901
R. M. PARR, Trace Element Analytical Chemistry in Medicine and Biology P. BRÄTTER, P. SCHRAMEL [Eds], W. De GRUYTER, New York, 1980, p. 631.
R. A. BYRNE, L. KOSTA, V. RAVNIK, J. STUPAR, V. HUDNIK, Nuclear Activation Techniques in the Life Sciences, 1978, IAEA, Vienna, 1979, p. 255.
Th. G. AALBERS, T. G. VERBURG, P. S. TJIOE, J. J. M. De GOEIJ, IRI Report 133-78-09, Delft, The Netherlands.
J. KUCERA, J. J. M. De GOEIJ, J. Radioanal. Chem., 63 (1981) 23.
G. V. IYENGAR, K. KASPEREK, L. E. FEINENDEGEN, Y. X. WYNG, H. WEESE, Sci. Total Environ., 24 (1982) 267.
J. J. M. De GOEIJ, L. KOSTA, A. R. BYRNE, J. KUCERA, Anal. Chim. Acta, 146 (1983) 161.
A. R. BYRNE, C. CAMARA-RICA, R. CORNELIS, J. J. M. De GOEIJ, G. V. IYENGAR, G. KIRKBRIGHT, G. KNAPP, R. M. PARR, M. STOEPPLER, Fresen. Z. Anal. Chem., 326 (1987) 723.
R. DYBCZYŃSKI, B. DANKO, J. KACZOROWSKI, Chem. Anal. (Warsaw) 34 (1989) No. 1.
R. DYBCZYŃSKI, H. MALESZEWSKA, Chem. Anal. (Warsaw) 32 (1987) 619.
R. DYBCZYŃSKI, H. MALESZEWSKA, M. WASEK, J. Radioanal. Nucl. Chem., 96 (1985) 187.
G. ERDTMANN, Neutron Activation Analysis Tables, Verlag Chemie, Weinheim, 1976.
V. C. ROGERS, Anal. Chem., 42 (1970) 807.
D. F. COVELL, Anal. Chem., 31 (1959) 1785.
S. STERLIŃSKI, Anal. Chem., 40 (1968) 1995.
L. A. CURRIE, Anal. Chem., 40 (1968) 586.
L. H. KEITH, W. CRUMMETT, J. DEEGAN Jr., R. A. LIBBY, J. K. TAYLOR, G. WENTLER, Anal. Chem., 55 (1983) 2210.
G. L. LONG, J. D. WINEFORDNER, Anal. Chem., 55 (1983) 713A.
National Bureau of Standards, Certificate of Analysis, Standard Reference Material 1577, Bovine Liver, Washington, D. C., 1972.
Progress Report No. 1, Intercomparison of Cadmium and other elements in IAEA Horse Kidney (H-8) IAEA, Vienna, 1985.
National Bureau of Standards, Certificate of Analysis, Standard Reference Material 1571, Orchard Leaves, Washington, D. C., 1977.
National Bureau of Standards Certificate of Analysis, Standard Reference Material 1570, Trace Elements in Spinach, Washington, D. C., 1976.
L. PSZONICKI, A. N. HANNA, Report on the Intercomparison IAEA (V-10) of the Determination of Trace Elements in Hay Powder, IAEA/RL/123, IAEA, Vienna, July 1985.
Report No. 26, Intercalibration of Analytical Methods of Marine Environmental Samples, Trace Element Measurements in Mussel Homogenate (MA-M-2/TM), IAEA/RL/126 (Monaco 26), IAEA, Monaco, October 1985.
IUPAC, Anal. Chem. Div., Pure Appl. Chem., 51 (1979) 1185.
R. DYBCZYŃSKI, A. VEGLIA, O. SUSCHNY, Report on the Intercomparison Run A-11 for the Determination of Inorganic Constituents of Milk Powder, IAEA/RL/68, IAEA, July, 1980.
H. S. DANG, H. B. DESAI, D. D. JAISWAL, S. R. KAYASTH, S. SOMASUNDRAM, J. Radioanal. Chem., 77 (1983) 65.
A. GHARIB, H. RAHIMI, H. PYROVAN, N. J. RAOFFI, H. TAHERPOOR, J. Radioanal. Chem., 89 (1985) 31.
J. J. M. De GOEIJ, personal communication, May 1980.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dybczyński, R., Wasek, M. & Maleszewska, H. A definitive method for the determination of small amounts of copper in biological materials by neutron activation analysis. Journal of Radioanalytical and Nuclear Chemistry, Articles 130, 365–388 (1989). https://doi.org/10.1007/BF02041356
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02041356