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Glycogenolytic action of glucagon-family peptides and epinephrine on catfish hepatocytes

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Abstract

Glycogenolytic effects of salmon and mammalian glucagons, salmon glucagon-like peptide (GLP) and epinephrine were studied on liver cells isolated from catfish (Ictalurus melas). In spring and summer, salmo-glucagon (3×10−10 to 3×10−8 M) was more effective than its mammalian counterpart in the stimulation of glucose release and cAMP synthesis in hepatocytes. GLP was less potent as compared to both glucagons. γ-amylase activity was not affected by the treatment with either glucagon-family peptides or epinephrine.

The comparison of the glycogenolytic effects of salmon glucagon to those of epinephrine reveals a greater potency of the latter hormone in the stimulation of cAMP synthesis, glycogen-phosphorylase activity and glucose release. Glycogen content in the liver cells was equally depleted after treatment with both of the two hormones.

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Authors and Affiliations

  1. Institute of General Physiology, University of Ferrara, Via L. Borsari 46, 44100, Ferrara, Italy

    A. C. Puviani, M. E. Gavioli, E. Fabbri & L. Brighenti

  2. Department of Zoology, University of Washington, 98195, Seatlle, WA, USA

    C. Ottolenghi & E. M. Plisetskaya

Authors
  1. C. Ottolenghi
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  2. A. C. Puviani
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  3. M. E. Gavioli
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  4. E. Fabbri
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  5. L. Brighenti
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  6. E. M. Plisetskaya
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Ottolenghi, C., Puviani, A.C., Gavioli, M.E. et al. Glycogenolytic action of glucagon-family peptides and epinephrine on catfish hepatocytes. Fish Physiol Biochem 6, 387–394 (1989). https://doi.org/10.1007/BF01875608

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