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Occurrence of 2-keto-3-deoxyoctonate (KDO) and KDO phosphate in lipopolysaccharides ofBacteriodes species

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Abstract

Occurrence of 2-keto-3-deoxyoctonate (KDO) in lipopolysaccharides (LPS) of genusBacteroides (some strains have recently been reclassified asPorphyromonas orPrevotella) was examined. Strong-acid treatment of LPS isolated fromBacteroides fragilis, Bacteroides (Porphyromonas) gingivalis andBacteroides intermedius, (Prevotella intermedia) released periodate/thiobarbituric acid reaction-positive substances that were not detectable under conventional hydrolysis conditions. These substances were demonstrated to be KDO phosphate by high voltage paper electrophoresis before and after alkaline phosphatase treatment. KDO phosphate was also identified in these LPS by gas-liquid chromatography and gas-liquid chromatography/mass spectrometry. KDO was identified as well in both mild and strong-acid hydrolysates of LPS isolated fromBacteriodes melaninogenicus (Prevotella melaninogenica). Neither KDO nor KDO phosphate was detectable in LPS ofBacteriodes asaccharolyticus (Porphyromonas asaccharolytica) even after the strong-acid treatment of LPS. These findings indicate that there are possible structural variations in the inner core region ofBacteroides LPS.

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Authors and Affiliations

  1. Department of Oral Microbiology, Kanagawa Dental College, 82 Inaoka-cho, 238, Yokosuka, Kanagawa, Japan

    Hidefumi Kumada &  Toshio Umemoto

  2. Division of Microbiology, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo, Japan

    Yuji Haishima

  3. Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan

    Seiichi Kondo & Kazuhito Hisatsune

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  1. Hidefumi Kumada
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  2. Yuji Haishima
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  3. Seiichi Kondo
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  4. Toshio Umemoto
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  5. Kazuhito Hisatsune
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Kumada, H., Haishima, Y., Kondo, S. et al. Occurrence of 2-keto-3-deoxyoctonate (KDO) and KDO phosphate in lipopolysaccharides ofBacteriodes species. Current Microbiology 26, 239–244 (1993). https://doi.org/10.1007/BF01577383

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