Summary
This paper describes a method to establish primary and secondary cultures of adipocyte precursors isolated from inguinal fat pads of 48-hr-old rats in defined medium. The culture medium consists of DME-F12 medium supplemented with fibronectin, insulin, transferrin, and fibroblast growth factor. Data presented indicate that 90% of the cells plated in the 4F medium differentiate in 7 d. These cultures provide appropriate differentiation assay systems to characterize regulators of differentiation acting on normal cells derived from the adipose tissue.
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Serrero, G., Mills, D. Assay for adipose differentiation using primary culture of adipocyte precursors. Journal of Tissue Culture Methods 10, 75–81 (1986). https://doi.org/10.1007/BF01404597
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DOI: https://doi.org/10.1007/BF01404597