Summary
Purified human cytomegalovirus nucleocapsids are able to transform supercoiled plasmid DNA into nicked circular DNA.
The activity exhibits a ionic strength optimum of 0.15 M; has a broad pH and temperature depencence and is enhanced after pre-incubation of the nucleocapsids with NP40.
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References
Bachi, B., Arber, W.: Physical mapping of BglII, BamHI, EcoRI, HindIII and PstI restriction fragments of bacteriophage P DNA. Molec. Gen. Genet.153, 311 to 324 (1977).
Bauer, W. R., Ressner, E. C., Kates, J., Patzke, J. V.: A DNA nicking-closing enzyme encapsidated in vaccinia virus: partial purification and properties. Proc. Natl. Acad. Sci. U.S.A.74, 1841–1845 (1977).
Champoux, J. J.: Protein that affect DNA conformation. Ann. Rev. Biochem.47, 449–479 (1978).
Geider, K., Hoffman-Berling, H.: Protein controlling the helical structure of DNA. Ann. Rev. Biochem.50, 233–260 (1981).
Gellert, M.: DNA topoisomerases. Ann. Rev. Biochem.50, 879–910 (1981).
Guerry, P., le Blanc, D. J., Falkow, S.: General method for the isolation of plasmid deoxyribonucleic acid. J. Bacteriol.116, 1064–1066 (1973).
Kamata, T., Tanaka, S., Watanabe, Y.: Human cytomegalovirus induced chromatin factors responsable for changes in template activity and structure of infected cell chromatin. Virology90, 197–208 (1978).
Kierszenbaum, A. L., Huang, E. S.: Chromatin pattern consisting of repeating bipartite structure in WI38 cells infected with human cytomegalovirus. J. Virol.38, 661–664 (1978).
Mar, E. C., Patel, P. C., Huang, E. S.: Human cytomegalovirus-associated DNA polymerase and protein kinase activities. J. gen. Virol.57, 149–156 (1981).
Musiani, M., Beltrame, F., Zerbini, M., Landini, M. P., Nicolini, C., la Placa, M.: Cytomegalovirus-induced early conformational changes of cell chromatin revealed by automated image analysis. Microbiologica4, 101–113 (1981).
Rosenberg, B. H., Ugers, G., Deutsch, J. F.: Variation in DNA-swivel enzyme activity during the mammalian cell cycle. Nucleic Acids Res.3, 3305–3311 (1976).
Smith, G. R.: DNA supercoiling: another level for regulating gene expression. Cell24, 599–600 (1981).
Stinski, M. F.: Glycoproteins associated with virions and dense bodies. J. Virol.19, 594–609 (1976).
Stinski, M. F.: Sequence of proteins and glycoproteins in cell infected with human cytomegalovirus. J. Virol.26, 686–701 (1978).
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Landini, M.P., Ripalti, A. A DNA-nicking activity associated with the nucleocapsid of human cytomegalovirus. Archives of Virology 73, 351–356 (1982). https://doi.org/10.1007/BF01318089
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DOI: https://doi.org/10.1007/BF01318089