Summary
Cultures of cell line IZD-MB 0503 from Mamestra brassicae were inoculated with nonoccluded nuclear polyhedrosis virus of Autographa californica (AcNOV). At 1 h postinoculation (p.i.) nucleocapsids were found in the cytoplasm near nuclear pores and within the nucleoplasm. Formation of virogenic stroma was observed at 7 h p.i. The first short empty capsids were seen at 10 h postinoculation (p.i.), followed by partially and completely filled nucleocapsids (11–12h p.i.) with most capsids filled at 12h and later. This suggests that nucleocapsid components, such as capsid and DNA-core, assemble in successive stages rather than simultaneously. Viral progeny, being released from the cells by budding, were observed at 13h p.i.
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Supported by Bundesministerium für Forschung und Technologie, Bonn, Kennzeichen PTB 8141
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Bassemir, U., Miltenburger, H.G. & David, P. Morphogenesis of nuclear polyhedrosis virus from Autographa californiea in a cell line from Mamestra brassicae (cabbage moth). Cell Tissue Res. 228, 587–595 (1983). https://doi.org/10.1007/BF00211478
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DOI: https://doi.org/10.1007/BF00211478