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Pseudotyped Virus for Papillomavirus

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Pseudotyped Viruses

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1407))

Abstract

Papillomavirus is difficult to culture in vitro, which limits its related research. The development of pseudotyped virus technology provides a valuable research tool for virus infectivity research, vaccine evaluation, infection inhibitor evaluation, and so on. Depending on the application fields, different measures have been developed to generate various kinds of pseudotyped papillomavirus. L1-based and L2-based HPV vaccines should be evaluated using different pseudotyped virus system. Pseudotyped papillomavirus animal models need high-titer pseudotyped virus and unique handling procedure to generate robust results. This paper reviewed the development, optimization, standardization, and application of various pseudotyped papillomavirus methods.

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Abbreviations

BM:

Basement membrane

BPV:

Bovine papillomavirus

CFP:

Crimson fluorescent protein

CPE:

Cytopathic effect

CRPV:

Cottontail rabbit papillomavirus

DNA:

Deoxyribonucleic acid

FC:

Furin-cleaved

GFP:

Green fluorescent protein

Gluc:

Gaussia luciferase

HPV:

Human papillomavirus

HSPG:

Heparan sulfate proteoglycan

ID50:

50% inhibitory dose

IVIS:

In vivo imaging system

MSD:

Merck Sharp & Dohme

N-9:

Nonoxynol-9

NMIBC:

Nonmuscular invasive bladder cancer

PBNA:

Pseudotyped virus based neutralization assay

PsV:

Pseudotyped virus

RFP:

Red fluorescent protein

RNA:

Ribonucleic acid

SEAP:

Secretory alkaline phosphatase

SFV:

Semliki Forest Virus

VLP:

Viruslike particle

WHO:

World Health Organization

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Acknowledgments

This work was supported by the General Program of National Natural Science Foundation of China [grant number 82172244&82073621] and a major project of the Study on Pathogenesis and Epidemic Prevention Technology System [2021YFC2302500].

Author information

Authors and Affiliations

  1. Cell Collection and Research Center, National Institutes for Food and Drug Control (NIFDC), and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China

    Xueling Wu

  2. Division of HIV/AIDS and Sex-transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC) and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China

    Jianhui Nie

  3. Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

    Youchun Wang

  4. Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Kunming, China

    Youchun Wang

Authors
  1. Xueling Wu
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  2. Jianhui Nie
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  3. Youchun Wang
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Corresponding authors

Correspondence to Jianhui Nie or Youchun Wang .

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Editors and Affiliations

  1. Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

    Youchun Wang

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Wu, X., Nie, J., Wang, Y. (2023). Pseudotyped Virus for Papillomavirus. In: Wang, Y. (eds) Pseudotyped Viruses. Advances in Experimental Medicine and Biology, vol 1407. Springer, Singapore. https://doi.org/10.1007/978-981-99-0113-5_5

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