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Screening Methods for the Evaluation of Antiplatelet Drugs

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Introduction to Basics of Pharmacology and Toxicology

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Abstract

Thrombosis is the key event in major cardiovascular and cerebrovascular events resulting in significant morbidity and mortality. Antiplatelet drugs like aspirin, clopidogrel inhibits platelet aggregation and inhibits thrombus formation. It is used widely in the primary and secondary prophylaxis of major cardiovascular and cerebrovascular events. Use of appropriate animal and model is utmost importance in preclinical evaluation because of the species difference variation in the event of a thrombosis. Various animals like rats, dogs, rabbits and cynomolgus monkey are used in the preclinical evaluation of antiplatelet drugs. Recently Zebrafish have been evolved as a useful model because of easiness in handle & care and resemblance of its genetics to humans. This chapter will discuss in details about various invitro and invivo model in the evaluation of the antiplatelet drugs.

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Bibliography

  • Andre P, Bal ditSollier C, Bonneau M, Pignaud G, Hainaud P, Azzam K, Drouet L. Which experimental model to choose to study arterial thrombosis and evaluate potentially useful therapeutics? Haemostasis. 1996;26(4):55–69.

    Google Scholar 

  • Andrews RK, Berndt MC. Platelet physiology and thrombosis. Thromb Res. 2004;114:447–53.

    Article  CAS  Google Scholar 

  • Bodary PF, Eitzman DT. Animal models of thrombosis. Curr Opin Hematol. 2009;16:342–6.

    Article  Google Scholar 

  • Chintala M, Strony J, Yang B, Kurowski S, Li Q. SCH 602539, a protease-activated receptor-1 antagonist, inhibits thrombosis alone and in combination with cangrelor in a folts model of arterial thrombosis in cynomolgus monkeys. ArteriosclerThrombVasc Biol. 2010;30:2143–9.

    Article  CAS  Google Scholar 

  • Dyszkiewicz-Korpanty A, Olteanu H, Frenkel EP, Sarode R. Clopidogrel anti-platelet effect: an evaluation by optical aggregometry, impedance aggregometry, and the platelet function analyzer (PFA-100). Platelets. 2007;18:491–6.

    Article  CAS  Google Scholar 

  • Gremmel T, Frelinger AL 3rd, Michelson AD. Platelet physiology. Semin Thromb Hemost. 2016;42:191–204.

    Article  CAS  Google Scholar 

  • Hajjarian Z, Tshikudi DM, Nadkarni SK. Evaluating platelet aggregation dynamics from laser speckle fluctuations. Biomed Opt Express. 2017;8:3502–15.

    Article  CAS  Google Scholar 

  • Jagadeeswaran P, Cooley BC, Gross PL, Mackman N. Animal models of thrombosis from zebrafish to nonhuman primates: use in the elucidation of new pathologic pathways and the development of antithrombotic drugs. Circ Res. 2016;118:1363–79.

    Article  CAS  Google Scholar 

  • Kamada H, Okamoto T, Hayashi T, Suzuki K. An in vitro method for screening anti-platelet agents using a microchannel array flow analyzer. Biorheology. 2010;47:153–61.

    Article  CAS  Google Scholar 

  • Koltai K, Kesmarky G, Feher G, Tibold A, Toth K. Platelet aggregometry testing: molecular mechanisms, techniques and clinical implications. Int J Mol Sci. 2017;18:1803. https://doi.org/10.3390/ijms18081803.

    Article  CAS  Google Scholar 

  • Kweon OJ, Lim YK, Kim B, Lee MK, Kim HR. Effectiveness of platelet function analyzer-100 for laboratory detection of anti-platelet drug-induced platelet dysfunction. Ann Lab Med. 2019;39:23–30.

    Article  CAS  Google Scholar 

  • Majithia A, Bhatt DL. Novel antiplatelet therapies for atherothrombotic diseases. ArteriosclerThrombVasc Biol. 2019;39:546–57.

    Article  CAS  Google Scholar 

  • Matsuno H, Tokuda H, Ishisaki A, Zhou Y, Kitajima Y, Kozawa O. P2Y12 receptors play a significant role in the development of platelet microaggregation in patients with diabetes. J Clin Endocrinol Metab. 2005;90:920–7.

    Article  CAS  Google Scholar 

  • McFadyen JD, Schaff M, Peter K. Current, and future antiplatelet therapies: emphasis on preserving haemostasis. Nat Rev Cardiol. 2018;15:181–91.

    Article  CAS  Google Scholar 

  • Mizuno T, Tsukiya T, Takewa Y, Tatsumi E. Differences in clotting parameters between species for preclinical large animal studies of cardiovascular devices. J Artif Organs. 2018;21:138–41.

    Article  CAS  Google Scholar 

  • Ogawa T, Hashimoto M, Niitsu Y, Jakubowski JA, Tani Y, Otsuguro K, Asai F, Sugidachi A. Effects of prasugrel, a novel P2Y(12) inhibitor, in rat models of cerebral and peripheral artery occlusive diseases. Eur J Pharmacol. 2009;612:29–34.

    Article  CAS  Google Scholar 

  • Olson JD, Fass DN, Bowie EJW, et al. Ristocetin-induced aggregation of gel filtered platelets: a study of von Willebrand's disease and the effect of aspirin. Throm Res. 1973;3:501–51.

    Article  CAS  Google Scholar 

  • Paniccia R, Priora R, Liotta AA, Abbate R. Platelet function tests: a comparative review. Vasc Health Risk Manag. 2015;11:133–48.

    Article  Google Scholar 

  • Pérez P, Alarcón M, Fuentes E, Palomo I. Thrombus formation induced by laser in a mouse model. Exp Ther Med. 2014;8:64–8.

    Article  Google Scholar 

  • Salzman EW, Rosenberg RD, Smith MH, Lindon JN, Favreau L. Effect of heparin and heparin fractions on platelet aggregation. J Clin Invest. 1980;65:64–73.

    Article  CAS  Google Scholar 

  • Shinozawa E, Kawamura M. Anti-thrombotic effect of a factor Xa inhibitor TAK-442 in a rabbit model of arteriovenous shunt thrombosis stimulated with tissue factor. BMC Res Notes. 2018;11:776. https://doi.org/10.1186/s13104-018-3886-4.

    Article  CAS  Google Scholar 

  • Westrick RJ, Winn ME, Eitzman DT. Murine model of thrombosis (Eitzman series). AteriosclerThombVasc Biol. 2007;27:2079–93.

    CAS  Google Scholar 

  • Wong PC, Watson C, Crain EJ. The P2Y1 receptor antagonist MRS2500 prevents carotid artery thrombosis in cynomolgus monkeys. J Thromb Thrombolysis. 2016;41:514–21.

    Article  CAS  Google Scholar 

  • Yeh JC, Huang KL, Hsiao YC, Hsu YH, Lin YH, Lou SL, Lee TH. A rat model of thrombosis in common carotid artery induced by implantable wireless light-emitting diode device. Biomed Res Int. 2014;2014:724134. https://doi.org/10.1155/2014/724134.

    Article  Google Scholar 

  • Yen TL, Wu MP, Chung CL, Yang WB, Jayakumar T, Geraldine P, Chou CM, Chang CY, Lu WJ, Sheu JR. Novel synthetic benzimidazole-derived oligosaccharide, M3BIM, prevents ex vivo platelet aggregation and in vivo thromboembolism. J Biomed Sci. 2016;23:26. https://doi.org/10.1186/s12929-016-0245-4.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Clinical Pharmacology, JIPMER, Puducherry, India

    Ilanthamizhan Jayakumar

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  1. Ilanthamizhan Jayakumar
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Editors and Affiliations

  1. Department of Pharmacology, Thanjavur Medical College, Thanjavur, Tamil Nadu, India

    Mageshwaran Lakshmanan

  2. Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, Pondicherry, India

    Deepak Gopal Shewade

  3. Department of Pharmacology, All India Institute of Medical Sciences (AIIMS) Bibinagar, Hyderabad, Telangana, India

    Gerard Marshall Raj

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Jayakumar, I. (2022). Screening Methods for the Evaluation of Antiplatelet Drugs. In: Lakshmanan, M., Shewade, D.G., Raj, G.M. (eds) Introduction to Basics of Pharmacology and Toxicology. Springer, Singapore. https://doi.org/10.1007/978-981-19-5343-9_25

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  • DOI: https://doi.org/10.1007/978-981-19-5343-9_25

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-19-5342-2

  • Online ISBN: 978-981-19-5343-9

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