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Spermatogonial Stem Cells and Testis-Tissue Cryopreservation as a Tool for Conservation of Buffalo Germplasm

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Biotechnological Applications in Buffalo Research

Abstract

Buffalo is an economically valuable animal of the south-east Asian counties, and they have made a significant contribution to livestock production. In recent days, due to the rapid decline of valuable breeds of buffalo and their genetic diversity, there is a great need to conserve their germplasm. Development of spermatogonial stem cell technology, testicular stem cell banking, testis-tissue cryopreservation, methods to salvage the cryopreserved-thawed tissues, and mature them in vitro and in vivo to retrieve fertilization-competent gametes are some of the methods which contributed significantly to the conservation of their germplasm. These methods are of specific significance to the juvenile and young buffalo males who die before contributing their genetic potential to the next generation. A combination of these bio-techniques would have substantial and feasible applications in the propagation of their germplasm. However, there is still a need to improve the current methods and introduce new technologies for germplasm conservation and dissemination. This chapter aims to discuss these modern biotechnological methods to provide readers with an insight into how these technologies can find application in buffalo germplasm conservation.

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Acknowledgement

The authors would like to thank all the present and past laboratory colleagues who contributed directly or indirectly to this article. The authors would also like to thank the Council for Scientific and Industrial Research, Govt. of India, for funding the research.

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Correspondence to Sandeep Goel .

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© 2022 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

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Devi, L., Goel, S. (2022). Spermatogonial Stem Cells and Testis-Tissue Cryopreservation as a Tool for Conservation of Buffalo Germplasm. In: Chauhan, M.S., Selokar, N. (eds) Biotechnological Applications in Buffalo Research. Springer, Singapore. https://doi.org/10.1007/978-981-16-7531-7_20

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