Abstract
The current world population is rapidly approaching the eight billion mark, and the current projected annual increase of 1.1% will take the population to approximately ten billion people in the year 2050. Therefore, maintaining and increasing our food production systems to feed the ever-growing population is a major concern. Abiotic and biotic stresses have increased in onset, duration, and intensity, and this could be attributed to more severe cycles of climate change. These stresses will severely hamper the future output and yield from crop plant-based agricultural systems all over the world. To improve yield, genetic engineering technologies have become an attractive tool for rapidly improving plant tolerance to abiotic and biotic stresses. For this purpose, proper bioinformatic gene mining tools have laid the foundation for selecting and identifying genes for downstream editing. This chapter describes some of the gene mining tools that led to selected genes for engineering plant tolerance to abiotic and biotic stresses in the four major food crop plant families.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Adhikari B, Adhikari M, Ghimire B et al (2019) Cold atmospheric plasma-activated water irrigation induces defense hormone and gene expression in tomato seedlings. Sci Rep 9(1):1–15. https://doi.org/10.1038/s41598-019-52646-z
Ait Issad H, Aoudjit R, Rodrigues JJPC (2019) A comprehensive review of Data Mining techniques in smart agriculture. Eng Agric Environ Food 12(4):511–525. https://doi.org/10.1016/j.eaef.2019.11.003
Andersson M, Turesson H, Nicolia A et al (2017) Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts. Plant Cell Rep 36(1):117–128. https://doi.org/10.1007/s00299-016-2062-3
Arshad M, Feyissa BA, Amyot L et al (2017) MicroRNA156 improves drought stress tolerance in alfalfa (Medicago sativa) by silencing SPL13. Plant Sci 258:122–136. https://doi.org/10.1016/j.plantsci.2017.01.018
Aung B, Gruber MY, Amyot L et al (2015) MicroRNA156 as a promising tool for alfalfa improvement. Plant Biotechnol J 13(6):779–790. https://doi.org/10.1111/pbi.12308
Bartoszewski G, Niedziela A, Szwacka M et al (2003) Modification of tomato taste in transgenic plants carrying a thaumatin gene from Thaumatococcus daniellii Benth. Plant Breed 122(4):347–351. https://doi.org/10.1046/j.1439-0523.2003.00864.x
Basu U, Narnoliya L, Srivastava R et al (2019) CLAVATA signaling pathway genes modulating flowering time and flower number in chickpea. Theor Appl Genet 132(7):2017–2038. https://doi.org/10.1007/s00122-019-03335-y
Batista-Silva W, Medeiros DB, Rodrigues-Salvador A et al (2019) Modulation of auxin signalling through DIAGETROPICA and ENTIRE differentially affects tomato plant growth via changes in photosynthetic and mitochondrial metabolism. Plant Cell Environ 42(2):448–465. https://doi.org/10.1111/pce.13413
Batley J, Edwards D (2016) The application of genomics and bioinformatics to accelerate crop improvement in a changing climate. Curr Opin Plant Biol 30:78–81. https://doi.org/10.1016/j.pbi.2016.02.002
Bhatnagar-Mathur P, Vadez V, Sharma KK (2008) Transgenic approaches for abiotic stress tolerance in plants: retrospect and prospects. Plant Cell Rep 27(3):411–424. https://doi.org/10.1007/s00299-007-0474-9
Blando F, Berland H, Maiorano G et al (2019) Nutraceutical characterization of anthocyanin-rich fruits produced by “sun black” tomato line. Front Nutr 6:133. https://doi.org/10.3389/fnut.2019.00133
Blanvillain-Baufumé S, Reschke M, Solé M et al (2017) Targeted promoter editing for rice resistance to Xanthomonas oryzae pv. oryzae reveals differential activities for SWEET14-inducing TAL effectors. Plant Biotechnol J 15(3):306–317. https://doi.org/10.1111/pbi.12613
Brekke TD, Stroud JA, Shaw DS et al (2019) QTL mapping in salad tomatoes. Euphytica 215(7):1–12. https://doi.org/10.1007/s10681-019-2440-3
Byrt CS, Grof CPL, Furbank RT (2011) C4 plants as biofuel feedstocks: optimising biomass production and feedstock quality from a lignocellulosic perspective. J Integr Plant Biol 53(2):120–135. https://doi.org/10.1111/j.1744-7909.2010.01023.x
Cai Y, Chen L, Liu X et al (2018) CRISPR/Cas9-mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean. Plant Biotechnol J 16(1):176–185. https://doi.org/10.1111/pbi.12758
Chen F, Dixon RA (2007) Lignin modification improves fermentable sugar yields for biofuel production. Nat Biotechnol 25(7):759–761. https://doi.org/10.1038/nbt1316
Chen H, Wang JP, Liu H et al (2019) Hierarchical transcription factor and chromatin binding network for wood formation in populus trichocarpa. Plant Cell 31(3):602–626. https://doi.org/10.1105/tpc.18.00620
Cheng Q, Li N, Dong L, Zhang D, Fan S, Jiang L, Wang X, Xu P, Zhang S (2015) Overexpression of soybean isoflavone reductase (GmIFR) enhances resistance to phytophthora sojae in soybean. Front Plant Sci 6:1–11. https://doi.org/10.3389/fpls.2015.01024
Chi X, Yang Q, Pan L et al (2011) Isolation and characterization of fatty acid desaturase genes from peanut (Arachis hypogaea L.). Plant Cell Rep 30(8):1393–1404. https://doi.org/10.1007/s00299-011-1048-4
Corbesier L, Vincent C, Jang S et al (2007) FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis. Science 316(5827):1030–1033. https://doi.org/10.1126/science.1141752
Cox DBT, Platt RJ, Zhang F (2015) Therapeutic genome editing: prospects and challenges. Nat Med 21(2):121–131. https://doi.org/10.1038/nm.3793
Dar AA, Choudhury AR, Kancharla PK et al (2017) The FAD2 gene in plants: occurrence, regulation, and role. Front Plant Sci 8:1789. https://doi.org/10.3389/fpls.2017.01789
Del Carmen Orozco-Mosqueda M, Duan J, DiBernardo M et al (2019) The production of ACC deaminase and trehalose by the plant growth promoting bacterium Pseudomonas sp. UW4 synergistically protect tomato plants against salt stress. Front Microbiol 10:1392. https://doi.org/10.3389/fmicb.2019.01392
Doyle EL, Booher NJ, Standage DS et al (2012) TAL effector-nucleotide targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction. Nucleic Acids Res 40(W1):W117. https://doi.org/10.1093/nar/gks608
Du H, Zeng X, Zhao M et al (2016) Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9. J Biotechnol 217:90–97. https://doi.org/10.1016/j.jbiotec.2015.11.005
Dutt S, Manjul AS, Raigond P et al (2017) Key players associated with tuberization in potato: potential candidates for genetic engineering. Crit Rev Biotechnol 37(7):942–957. https://doi.org/10.1080/07388551.2016.1274876
Feingold S, Lloyd J, Norero N et al (2005) Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.). Theor Appl Genet 111(3):456–466. https://doi.org/10.1007/s00122-005-2028-2
Filiz E, Ozyigit II, Saracoglu IA et al (2019) Abiotic stress-induced regulation of antioxidant genes in different Arabidopsis ecotypes: microarray data evaluation. Biotechnol Biotechnol Equip 33(1):128–143. https://doi.org/10.1080/13102818.2018.1556120
Fletcher JC (2018) The CLV-WUS stem cell signaling pathway: a roadmap to crop yield optimization. Plan Theory 7(4). https://doi.org/10.3390/plants7040087
Furbank RT, Tester M (2011) Phenomics - technologies to relieve the phenotyping bottleneck. Trends Plant Sci 16(12):635–644. https://doi.org/10.1016/j.tplants.2011.09.005
Gokul A, Niekerk L-A, Carelse MF et al (2020) Transgenic technology for efficient abiotic stress tolerance in plants. In: Transgenic technology based value addition in plant biotechnology, pp 95–122
Gonda I, Ashrafi H, Lyon DA et al (2019) Sequencing-based bin map construction of a tomato mapping population, facilitating high-resolution quantitative trait loci detection. Plant Genome 12(1):180010. https://doi.org/10.3835/plantgenome2018.02.0010
Guo M, Rupe MA, Wei J et al (2014) Maize ARGOS1 (ZAR1) transgenic alleles increase hybrid maize yield. J Exp Bot 65(1):249–260. https://doi.org/10.1093/jxb/ert370
Hays DB, Do JH, Mason RE et al (2007) Heat stress induced ethylene production in developing wheat grains induces kernel abortion and increased maturation in a susceptible cultivar. Plant Sci 172(6):1113–1123. https://doi.org/10.1016/j.plantsci.2007.03.004
Huang H, Cui T, Zhang L et al (2020) Modifications of fatty acid profile through targeted mutation at BnaFAD2 gene with CRISPR/Cas9-mediated gene editing in Brassica napus. Theor Appl Genet 133:2401–2411. https://doi.org/10.1007/s00122-020-03607-y
Janila P, Pandey MK, Shasidhar Y et al (2016) Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes. Plant Sci 242:203–213. https://doi.org/10.1016/j.plantsci.2015.08.013
Jiang YN, Wang B, Li H et al (2010) Flavonoid production is effectively regulated by RNAi interference of two flavone synthase genes from glycine max. J Plant Biol 53(6):425–432. https://doi.org/10.1007/s12374-010-9132-9
Jiang Y, Hu Y, Wang B et al (2014) Bivalent RNA interference to increase isoflavone biosynthesis in soybean (glycine max). Brazilian Arch Biol Technol 57(2):163–170. https://doi.org/10.1590/S1516-89132013005000018
Kang SG, Hannapel DJ (1995) Nucleotide sequences of novel potato (Solanum tuberosum L.) MADS-box cDNAs and their expression in vegetative organs. Gene 166(2):329–330. https://doi.org/10.1016/0378-1119(95)00593-5
Kannan B, Jung JH, Moxley GW et al (2018) TALEN-mediated targeted mutagenesis of more than 100 COMT copies/alleles in highly polyploid sugarcane improves saccharification efficiency without compromising biomass yield. Plant Biotechnol J 16(4):856–866. https://doi.org/10.1111/pbi.12833
Kawakami EM, Oosterhuis DM, Snider JL (2010) Physiological effects of 1-methylcyclopropene on well-watered and water-stressed cotton plants. J Plant Growth Regul 29(3):280–288. https://doi.org/10.1007/s00344-009-9134-3
Khanna HK, Deo PC (2016) Novel gene transfer technologies. In: Banana: genomics and transgenic approaches for genetic improvement. Springer, Singapore, pp 127–140
Kong F, Liu B, Xia Z et al (2010) Two coordinately regulated homologs of FLOWERING LOCUS T are involved in the control of photoperiodic flowering in Soybean. Plant Physiol 154(3):1220–1231. https://doi.org/10.1104/pp.110.160796
Kumar K, Kumar M, Kim SR et al (2013) Insights into genomics of salt stress response in rice. Rice 6(1):1–15. https://doi.org/10.1186/1939-8433-6-27
Lei Y, Lu L, Liu HY et al (2014) CRISPR-P: a web tool for synthetic single-guide RNA design of CRISPR-system in plants. Mol Plant 7(9):1494–1496. https://doi.org/10.1093/mp/ssu044
Lemke P, Moerschbacher BM, Singh R (2020) Transcriptome analysis of solanum tuberosum genotype RH89-039-16 in response to chitosan. Front Plant Sci 11:1193. https://doi.org/10.3389/fpls.2020.01193
Li M, Li X, Zhou Z et al (2016) Reassessment of the four yield-related genes Gn1a, DEP1, GS3, and IPA1 in rice using a CRISPR/Cas9 system. Front Plant Sci 7:377. https://doi.org/10.3389/fpls.2016.00377
Li J, Sun Y, Du J et al (2017) Generation of targeted point mutations in rice by a modified CRISPR/Cas9 system. Mol Plant 10(3):526–529. https://doi.org/10.1016/j.molp.2016.12.001
Li Q, Qin Y, Hu X et al (2020) Transcriptome analysis uncovers the gene expression profile of salt-stressed potato (Solanum tuberosum L.). Sci Rep 10(1):1–19. https://doi.org/10.1038/s41598-020-62057-0
Liao G, He Y, Li X et al (2019) Effects of bagging on fruit flavor quality and related gene expression of AsA synthesis in Actinidia eriantha. Sci Hortic 256:108511. https://doi.org/10.1016/j.scienta.2019.05.038
Liu CJ, Blount JW, Steele CL et al (2002) Bottlenecks for metabolic engineering of isoflavone glycoconjugates in Arabidopsis. Proc Natl Acad Sci U S A 99(22):14578–14583. https://doi.org/10.1073/pnas.212522099
Liu C, Chen L, Zhao R et al (2019) Melatonin induces disease resistance to botrytis cinerea in tomato fruit by activating jasmonic acid signaling pathway. J Agric Food Chem 67(22):6116–6124. https://doi.org/10.1021/acs.jafc.9b00058
Liu S, Li C, Wang H et al (2020) Mapping regulatory variants controlling gene expression in drought response and tolerance in maize. Genome Biol 21(1):1–22. https://doi.org/10.1186/s13059-020-02069-1
Lou D, Wang H, Liang G et al (2017) OsSAPK2 confers abscisic acid sensitivity and tolerance to drought stress in rice. Front Plant Sci 8:993. https://doi.org/10.3389/fpls.2017.00993
Ma X, Zhang Q, Zhu Q et al (2015) A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant 8(8):1274–1284. https://doi.org/10.1016/j.molp.2015.04.007
Ma C, Zhu C, Zheng M et al (2019) CRISPR/Cas9-mediated multiple gene editing in Brassica oleracea var. capitata using the endogenous tRNA-processing system. Hortic Res 6(1):1–15. https://doi.org/10.1038/s41438-018-0107-1
Mélida H, Bacete L, Ruprecht C et al (2020) Arabinoxylan-oligosaccharides act as damage associated molecular patterns in plants regulating disease resistance. Front Plant Sci 11:1210. https://doi.org/10.3389/fpls.2020.01210
Muñoz-Sanz JV, Zuriaga E, Cruz-García F et al (2020) Self-(In)compatibility systems: target traits for crop-production, plant breeding, and biotechnology. Front Plant Sci 11:195. https://doi.org/10.3389/fpls.2020.00195
Murovec J, Guček K, Bohanec B et al (2018) DNA-free genome editing of brassica oleracea and B. Rapa protoplasts using CRISPR-cas9 ribonucleoprotein complexes. Front. Plant Sci 871(1594). https://doi.org/10.3389/fpls.2018.01594
Naing AH, Kyu SY, Pe PPW et al (2019) Silencing of the phytoene desaturase (PDS) gene affects the expression of fruit-ripening genes in tomatoes. Plant Methods 15(1):1–10. https://doi.org/10.1186/s13007-019-0491-z
Nesbitt TC, Tanksley SD (2001) fw2. 2 directly affects the size of developing tomato fruit, with secondary effects on fruit number and photosynthate distribution. Plant Physiol 127(2):575–583. https://doi.org/10.1104/pp.010087
Ogawa T, Kawahigashi H, Toki S et al (2008) Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker. Plant Cell Rep 27(8):1325–1331. https://doi.org/10.1007/s00299-008-0553-6
Okamoto M, Tanaka Y, Abrams SR et al (2009) High humidity induces abscisic acid 8′-hydroxylase in stomata and vasculature to regulate local and systemic abscisic acid responses in Arabidopsis. Plant Physiol 149(2):825–834. https://doi.org/10.1104/pp.108.130823
Okuzaki A, Ogawa T, Koizuka C et al (2018) CRISPR/Cas9-mediated genome editing of the fatty acid desaturase 2 gene in Brassica napus. Plant Physiol Biochem 131:63–69. https://doi.org/10.1016/j.plaphy.2018.04.025
Pandey SP, Somssich IE (2009) The role of WRKY transcription factors in plant immunity. Plant Physiol 150(4):1648–1655. https://doi.org/10.1104/pp.109.138990
Pechanova O, Pechan T (2017) Proteomics as a tool to understand maize biology and to improve maize crop. In: Proteomics in food science: from farm to fork. Elsevier, pp 35–56
Pimentel D, Pimentel M (2003) Sustainability of meat-based and plant-based diets and the environment. Am J Clin Nutr 78:660–663
Qin H, Li Y, Huang R (2020) Advances and challenges in the breeding of salt-tolerant rice. Int J Mol Sci 21(21):1–15. https://doi.org/10.3390/ijms21218385
Quinet M, Angosto T, Yuste-Lisbona FJ et al (2019) Tomato fruit development and metabolism. Front Plant Sci 10:1554. https://doi.org/10.3389/fpls.2019.01554
Ray SK, Macoy DM, Kim WY et al (2019) Role of RIN4 in regulating PAMP-triggered immunity and effector-triggered immunity: current status and future perspectives. Mol Cells 42(7):503–511. https://doi.org/10.14348/molcells.2019.2433
Robinson BR, Salinas CG, Parra PR et al (2019) Expression levels of the γ-glutamyl hydrolase I gene predict Vitamin B9 content in potato tubers. Agronomy 9(11):734. https://doi.org/10.3390/agronomy9110734
Romero P, Rose JKC (2019) A relationship between tomato fruit softening, cuticle properties and water availability. Food Chem 295:300–310. https://doi.org/10.1016/j.foodchem.2019.05.118
Roth M, Florez-Rueda AM, Städler T (2019) Differences in effective ploidy drive genome-wide endosperm expression polarization and seed failure in wild tomato hybrids. Genetics 212(1):141–152. https://doi.org/10.1534/genetics.119.302056
Rothan C, Diouf I, Causse M (2019) Trait discovery and editing in tomato. Plant J 97(1):73–90. https://doi.org/10.1111/tpj.14152
Schiessl K, Muiño JM, Sablowski R (2014) Arabidopsis JAGGED links floral organ patterning to tissue growth by repressing Kip-related cell cycle inhibitors. Proc Natl Acad Sci U S A 111(7):2830–2835. https://doi.org/10.1073/pnas.1320457111
Serrani JC, Ruiz-Rivero O, Fos M et al (2008) Auxin-induced fruit-set in tomato is mediated in part by gibberellins. Plant J 56(6):922–934. https://doi.org/10.1111/j.1365-313X.2008.03654.x
Sevestre F, Facon M, Wattebled F et al (2020) Facilitating gene editing in potato: a Single-Nucleotide Polymorphism (SNP) map of the Solanum tuberosum L. cv. Desiree genome. Sci Rep 10(1). https://doi.org/10.1038/s41598-020-58985-6
Shao G, Xie L, Jiao G et al (2017) CRISPR/CAS9-mediated editing of the fragrant gene Badh2 in rice. Chin J Rice Sci 31(2):216–222. https://doi.org/10.16819/j.1001-7216.2017.6098
Shi J, Habben JE, Archibald RL et al (2015) Overexpression of ARGOS genes modifies plant sensitivity to ethylene, leading to improved drought tolerance in both arabidopsis and maize. Plant Physiol 169(1):266–282. https://doi.org/10.1104/pp.15.00780
Shi J, Drummond BJ, Wang H et al (2016) Maize and Arabidopsis ARGOS proteins interact with ethylene receptor signaling complex, supporting a regulatory role for ARGOS in ethylene signal transduction. Plant Physiol 171(4):2783–2797. https://doi.org/10.1104/pp.16.00347
Shi J, Gao H, Wang H et al (2017) ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions. Plant Biotechnol J 15(2):207–216. https://doi.org/10.1111/pbi.12603
Sun H, Jia Z, Cao D et al (2011) GmFT2a, a soybean homolog of flowering locus T, is involved in flowering transition and maintenance. PLoS One 6(12):e29238. https://doi.org/10.1371/journal.pone.0029238
Sun Z, Li N, Huang G et al (2013) Site-specific gene targeting using transcription activator-like effector (TALE)-based nuclease in Brassica oleracea. J Integr Plant Biol 55(11):1092–1103. https://doi.org/10.1111/jipb.12091
Sun Q, Lin L, Liu D et al (2018) CRISPR/Cas9-mediated multiplex genome editing of the BnWRKY11 and BnWRKY70 genes in brassica napus L. Int J Mol Sci 19(9). https://doi.org/10.3390/ijms19092716
Sun BR, Fu CY, Fan ZL et al (2019) Genomic and transcriptomic analysis reveal molecular basis of salinity tolerance in a novel strong salt-tolerant rice landrace Changmaogu. Rice 12(1):1–15. https://doi.org/10.1186/s12284-019-0360-4
Takagi H, Tamiru M, Abe A et al (2015) MutMap accelerates breeding of a salt-tolerant rice cultivar. Nat Biotechnol 33(5):445–449. https://doi.org/10.1038/nbt.3188
Teixeira PJP, Colaianni NR, Fitzpatrick CR et al (2019) Beyond pathogens: microbiota interactions with the plant immune system. Curr Opin Microbiol 49:7–17. https://doi.org/10.1016/j.mib.2019.08.003
Tieman D, Taylor M, Schauer N et al (2006) Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde. Proc Natl Acad Sci U S A 103(21):8287–8292. https://doi.org/10.1073/pnas.0602469103
Tiwari V, Chaturvedi AK, Mishra A et al (2015) An efficient method of agrobacterium-mediated genetic transformation and regeneration in local indian cultivar of groundnut (Arachis hypogaea) using grafting. Appl Biochem Biotechnol 175(1):436–453. https://doi.org/10.1007/s12010-014-1286-3
Tiwari JK, Devi S, Ali N et al (2018) Progress in somatic hybridization research in potato during the past 40 years. Plant Cell Tissue Organ Cult 132(2):225–238. https://doi.org/10.1007/s11240-017-1327-z
Tiwari JK, Devi S, Buckseth T et al (2020) Precision phenotyping of contrasting potato (Solanum tuberosum L.) varieties in a novel aeroponics system for improving nitrogen use efficiency: in search of key traits and genes. J Integr Agric 19(1):51–61. https://doi.org/10.1016/S2095-3119(19)62625-0
Upadhyaya DC, Bagri DS, Upadhyaya CP et al (2020) Genetic engineering of potato (Solanum tuberosum L.) for enhanced α-tocopherols and abiotic stress tolerance. Physiol Plant. https://doi.org/10.1111/ppl.13252
Vardi M, Levy NS, Levy AP (2013) Vitamin e in the prevention of cardiovascular disease: the importance of proper patient selection. J Lipid Res 54(9):2307–2314. https://doi.org/10.1194/jlr.R026641
Wani SH, Sah SK, Hossain MA et al (2016) Transgenic approaches for abiotic stress tolerance in crop plants. In: Advances in plant breeding strategies: agronomic, abiotic and biotic stress traits, vol 2. Springer, pp 345–396
Wen S, Liu H, Li X et al (2018) TALEN-mediated targeted mutagenesis of fatty acid desaturase 2 (FAD2) in peanut (Arachis hypogaea L.) promotes the accumulation of oleic acid. Plant Mol Biol 97(1–2):177–185. https://doi.org/10.1007/s11103-018-0731-z
Weng JK, Li X, Bonawitz ND et al (2008) Emerging strategies of lignin engineering and degradation for cellulosic biofuel production. Curr Opin Biotechnol 19(2):166–172. https://doi.org/10.1016/j.copbio.2008.02.014
Whaley CM, Wilson HP, Westwood JH (2007) A new mutation in plant ALS confers resistance to five classes of ALS-inhibiting herbicides. Weed Sci 55(2):83–90. https://doi.org/10.1614/ws-06-082.1
Xie K, Zhang J, Yang Y (2014) Genome-wide prediction of highly specific guide RNA spacers for CRISPR-Cas9-mediated genome editing in model plants and major crops. Mol Plant 7(5):923–926. https://doi.org/10.1093/mp/ssu009
Yang Y, Zhu K, Li H et al (2018) Precise editing of CLAVATA genes in Brassica napus L. regulates multilocular silique development. Plant Biotechnol J 16(7):1322–1335. https://doi.org/10.1111/pbi.12872
Yarra R (2020) Plastome engineering in vegetable crops: current status and future prospects. Mol Biol Rep 47(10):8061–8074. https://doi.org/10.1007/s11033-020-05770-3
Yi L, Chen C, Yin S et al (2018) Sequence variation and functional analysis of a FRIGIDA orthologue (BnaA3.FRI) in Brassica napus. BMC Plant Biol 18(1):1–13. https://doi.org/10.1186/s12870-018-1253-1
Zaman QU, Chu W, Hao M et al (2019) CRISPR/Cas9-mediated multiplex genome editing of jagged gene in brassica napus L. Biomol Ther 9(11). https://doi.org/10.3390/biom9110725
Zeng Z, Han N, Liu C et al (2020) Functional dissection of HGGT and HPT in barley vitamin e biosynthesis via CRISPR/Cas9-enabled genome editing. Ann Bot 126(5):929–942. https://doi.org/10.1093/AOB/MCAA115
Zhang A, Liu Y, Wang F et al (2019a) Enhanced rice salinity tolerance via CRISPR/Cas9-targeted mutagenesis of the OsRR22 gene. Mol Breed 39(3):1–10. https://doi.org/10.1007/s11032-019-0954-y
Zhang R, Liu J, Chai Z et al (2019b) Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing. Nat Plants 5(5):480–485. https://doi.org/10.1038/s41477-019-0405-0
Zhang P, Du H, Wang J et al (2020) Multiplex CRISPR/Cas9-mediated metabolic engineering increases soya bean isoflavone content and resistance to soya bean mosaic virus. Plant Biotechnol J 18(6):1384–1395. https://doi.org/10.1111/pbi.13302
Zhao J, Chi Y, Zhang XJ et al (2019) Implication of whitefly vesicle associated membrane protein-associated protein B in the transmission of Tomato yellow leaf curl virus. Virology 535:210–217. https://doi.org/10.1016/j.virol.2019.07.007
Acknowledgments
Miss. Niekerk, Mr. Carelse, and Dr. Bakare were financially supported by the National Research Foundation (NRF) of South Africa (Grant numbers: 111871 and 113857). Prof. Keyster’s research was financially supported by the NRF (Grant numbers: 116346 and 109083), DST-NRF Centre of Excellence in Food Security (Project ID: 170202), GrainSA (GB0200066), and the University of the Western Cape (UWC). Dr. Klein research was financially supported by the NRF (Grant numbers: 107023 and 115280), GrainSA (GB0200065), and UWC. Dr. Gokul’s research was financially supported by the NRF (Grant number: 129493) and the University of Free State (UFS). Therefore, the authors would like to express sincere gratitude toward these funding bodies.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Niekerk, LA., Carelse, M.F., Bakare, O., Klein, A., Gokul, A., Keyster, M. (2022). Application of Gene Mining and Editing Technologies for Agricultural Research and Breeding. In: Kamaluddin, Kiran, U., Abdin, M.Z. (eds) Technologies in Plant Biotechnology and Breeding of Field Crops. Springer, Singapore. https://doi.org/10.1007/978-981-16-5767-2_3
Download citation
DOI: https://doi.org/10.1007/978-981-16-5767-2_3
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-16-5766-5
Online ISBN: 978-981-16-5767-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)