Abstract
Self-assemblies of arginine-containing dendritic lipopeptides provide remarkable benefits for gene delivery through the virus-inspired design and fabrication. The present protocol focuses on synthesis, preparation, characterization of the gene carriers, including gene transfection efficiency evaluation as well as intracellular tracking. The synthesis of dendritic lipopeptides with bio-reducible disulfide linkage and dual aliphatic chains was firstly described as the basic compound, followed by a series of integrated co-delivery modifications with hydrophobic drugs and fluorescent probe. The obtained results demonstrated that the fabricated nanovectors exhibited high gene transfection efficiency, specific turn-on fluorescence imaging in tumors, and fast drug release from the dendritic arginine peptide-prodrug conjugates.
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Chen, X., Jin, R., Nie, Y. (2022). Preparation and Evaluation of Lipopeptides with Arginine-Rich Periphery for Gene Delivery. In: Tian, H., Chen, X. (eds) Gene Delivery. Biomaterial Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-16-5419-0_6
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DOI: https://doi.org/10.1007/978-981-16-5419-0_6
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