Abstract
This article summarizes recent findings with our supernodulation (nts) and nonnodulation (nod) mutants of soybean. The nonnodulation mutant nod 139 (unable to curl root hairs and to develop subepidermal cell divisions) does not induce the autoregulation response that controls nodulation. In contrast, nonnodulation mutant nod49 (also unable to curl root hairs, but able to induce some cortical cell divisions) induces autoregulation as effectively as the wild type. Since we perceive autoregulation of nodulation to be an arrest of nodule development, we postulate that cell division associated events signal the shoot of the plant of their activity and that the shoot responds by restricting their further development. We discovered a second nodulation control mechanism, recognized by nodule excision, which acts independently of, and after the autoregulation mechanism, and is different in soybean and alfalfa. Using RFLP mapping we were able to position the nts locus on linkage group F. We are using chromosome walking strategies to clarify the relation between the genetic and physical distance between closely linked loci. Nodules formed in the absence of Rhizobium (Nar) share functional and structural properties with Rhizobium-induced nodules. Alfalfa plants with 100% Nar progeny were selected. Observations like the above suggest that the plant controls the entire nodulation ontogeny and that the bacterium has evolved to activate the process in restrictive genotypes.
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References
Buzzell, R.I., Buttery, B.R., and Ablett, G. (1990) Supernodulation mutants in Elgin 87 soybean. in P.M. Gresshoff, G. Stacey, L.E. Roth and W.E. Newton (eds.) Nitrogen Fixation: Achievements and Objectives. Chapman Hall, New York. p. 726.
Caetano-Anollés, G., and Gresshoff, P.M. (1990) Early induction of feedback regulatory responses governing nodulation in soybean (G. max (L) Merr.) Plant Science, in press.
Caetano-Anollés, G., and Gresshoff, P.M. (1991) Feedback regulation of nodule formation in alfalfa. mature nodules control infection initiation. Plant Physiol. submitted.
Caetano-Anollés, G., Paparozzi, E. T., and Gresshoff, P.M.(1991) Mature nodules and root tips control nodulation in soybean. J. Plant Physiol., in press.
Caetano-Anollés, G., Joshi, P.A., and Gresshoff, P.M. (1990) Spontaneous nodules induce feedback suppression of nodulation in alfalfa. Planta, in press.
Gresshoff, P.M., Krotzky, A., Mathews, A., Day, D.A., Schuller, K.A., Olsson, J.E., Delves, A.C., and Carroll, B.J. (1988) Suppression of symbiotic supernodulation symptoms in soybeans. J. Plant Physiol. 132, 417–423.
Delves, A.C., Carroll, B. J., and Gresshoff, P.M. (1988) Genetic analysis and complementation studies on a number of mutant supernodulating soybean lines. J. Genetics 67, 1–8.
Delves, A.C., Mathews, A., Day, D.A., Carter, A.S., Carroll, B.J. and Gresshoff, P.M. (1986) Regulation of the soybean-Rhizobium symbiosis by shoot and root factors. Plant Physiol. 82, 588–590.
Funke, R., Sayavedra-Soto, L.A. and Gresshoff, P.M. (1991) Physical mapping of the supernodulation (nts) region of the soybean genome using pulse field gel electrophoresis. Mol. Gen. Genet. submitted.
Gremaud, M.F. and Harper, J.E. (1988) Selection and initial characterization of partially nitrate tolerant nodulation mutants of soybean. Plant Physiol. 89, 169–173.
Gresshoff, P.M. and Delves, A.C. (1986) Plant genetic approaches to symbiotic nodulation and nitrogen fixation in legumes. in A.D. Blonstein and P.J. King (eds.): Plant Gene Research III. A genetical approach to plant biochemistry, pp. 159–206. Springer Verlag. Wien.
Joshi, P.A., Caetano-Anollés, G., Graham, E., and Gresshoff, P.M. (1991) Ontogeny and ultrastructure of spontaneous nodules in alfalfa (Medicago sativum) Protoplasma, submitted.
Landau-Ellis, D., Shoemaker, R. and Gresshoff, P.M. (1990) Molecular mapping of the soybean supernodulation locus to RFLP linkage group F. Nature, submitted.
Lerouge, P., Roche, P., Faucher, C., Maillet, F., Truchet, G., Promé, Je., and Dénarié, J. (1990) Symbiotic host specificity of Rhizobium meliloti is determined by a sulphated and acylated glucosamine oligosaccharide signal. Nature 344, 781–784.
Mathews, A. 1989. PhD thesis, ANU, Canberra, Australia.
Mathews, A., Carroll, B.J., and Gresshoff, P.M. (1990) The genetic interaction between nonnodulation and supernodulation in soybean: an example of developmental epistasis. Theor. Appl. Genetics 79, 125–130.
Mathews, A., Carroll, B.J., Gresshoff, P.M. (1989a) Development of Bradyrhizobium infections in supernodulating and non-nodulating mutants of soybean (G. max (L.) Merrill). Protoplasma 150, 40–47.
Mathews, A., Kosslak, R.M., Sengupta-Gopalan, C. Appelbaum, E.R., Carroll, B.J. and Gresshoff, P.M. (1989b) Biological characterization of root exudates and extracts from nonnodulating and supernodulating soybean mutants. MPMI 2, 283–290.
Mathews, A., Carroll, B.J. and Gresshoff, P.M. (1989c) A new recessive gene conditioning non-nodulation in soybean. J. Hered. 80, 357–360.
Olsson, J.E., Nakao, P., Bohlool, B.B. and Gresshoff, P.M. (1989) Lack of systemic suppression of nodulation in split root systems of supernodulating soybean (Glycine max (L.) Merr.) mutants. Plant Physiol. 90, 1347–1352.
Rolfe, BG and Gresshoff, P.M. (1988) Genetic analysis of legume nodule initiation. Ann. Rev. Plant Physiol. Plant Mol. Biol. 39, 297–319.
Sayavedra-Soto, L.A., Angermüller, S.A., Prabdu, R., and Gresshoff, P.M. (1991) Polypeptide patterns in leaves of Glycine max (L) Merr. cv. Bragg and its supernodulation mutant during nodulation. The New Biologist. submitted.
Sutherland, T.D., Bassam, B.J., Schuller, L.J., Gresshoff, P.M. (1990) Early nodulation signals in wild type and symbiotic mutants of soybean (Glycine max (L.) Merr. cv. Bragg). MPMI 3,122–128.
Truchet, G., Vasse, J., Odorico, R., de Billy, F., Camut, S., and Huguet, T. (1988) Discrimination between nodules and root-derived structures: Does alfalfa nodulate spontaneously? in R. Palacios, and D.P.S. Verma (eds) : Molec. Genet. of Plant-Microbe Interactions. pp. 179–180.
Truchet, G., Barker, D. G., Camut, S., de Billy, F., Vasse, J., and Huguet, T. (1989) Alfalfa nodulation in the absence of Rhizobium. Mol. Gen. Genet. 219, 65–68.
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© 1991 Springer Science+Business Media Dordrecht
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Gresshoff, P.M., Landau-Ellis, D., Funke, R., Sayavedra-Soto, L., Caetano-Anolles, G. (1991). Plant Genetic Control of Nodulation in Legumes. In: Hennecke, H., Verma, D.P.S. (eds) Advances in Molecular Genetics of Plant-Microbe Interactions Vol. 1. Current Plant Science and Biotechnology in Agriculture, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-7934-6_51
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DOI: https://doi.org/10.1007/978-94-015-7934-6_51
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