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Monoclonal Antibodies and the Thyrotropin Receptor

  • Chapter
Monoclonal Antibodies to Receptors

Part of the book series: Receptors and Recognition ((SERB,volume 17))

Abstract

Thyrotropin (TSH) is a pituitary glycoprotein hormone whose primary role is to regulate thyroid cell function (Dumont, 1971; Field, 1980; Robbins et al., 1980; Kohn et al., 1983). The interaction of TSH with a specific receptor on the thyroid cell surface induces changes in adenylate cylcase activity which result in the following tissue responses: enhanced iodide uptake, thyroglobulin biosynthesis, iodination of thyroglobulin, degradation of iodinated thyroglobulin to form thyroid hormone, and the release of thyroid hormone (T3 and T4) into the bloodstream. Although changes in all of the above activities have been and can be used to define receptor function, definition of the structure of the TSH receptor on a molecular level, as with all other receptors, has required the identification of specific membrane components using binding studies and 125I-labeled hormone (Kohn, 1978; Kohn and Shifrin, 1982; Kohn et al., 1983).

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Abbreviations

TSH:

Thyrotropin (thyroid-stimulating hormone)

hCG:

Human chorionic gonadotropin

LH:

Luteinizing hormone

FSH:

Follicle-stimulating hormone

LIS:

Lithium diiodosalicylate

FRTL-5 cells:

Functioning rat thyroid cells derived from normal Fisher rat thyroid glands

GM1 :

Galactosyl-N-acetylgalactosaminyl-[N-acetylneuraminyl]galactosyl-glucosylceramide

GM2 :

N-Acetylgalactosaminyl-[N-acetylneuraminyl]galactosylglucosylceramide

GM3 :

N-Acetylneuraminylgalactosylglucosylceramide

GDla :

N-Acetylneuraminylgalactosyl-N-acetylgalactosaminyl-N-acetylneuraminyl]galactosylglucosylceramide

GDlb :

Galactosyl-N-acetylgalactosaminyl-[N-acerylneuraminyl-N-acetylneuraminyl]galactosylglucosylceramide

GT1 :

N-Acetylneuraminylgalactosyl-N-acetylgalactosaminyl-[N-acetylneuraminyl-N-acetylneuraminyl]galactosylglucosylceramide

T3 :

Tri-iodothyronine

T4 :

Thyroxine

CBA:

Cytochemical bioassay

TSAb:

Thyroid-stimulating antibody

FCCP:

Carbonyl cyanide p-trifluoromethoxyphenylhydrazone

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Authors
  1. Leonard D. Kohn
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  2. Donatella Tombaccini
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  3. Michele L. De Luca
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  4. Maurizio Bifulco
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  5. Evelyn F. Grollman
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  6. William A. Valente
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Editor information

Editors and Affiliations

  1. Leukaemia Research Fund Centre, Institute for Cancer Research, London, UK

    M. F. Greaves

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© 1984 Chapman and Hall

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Kohn, L.D., Tombaccini, D., De Luca, M.L., Bifulco, M., Grollman, E.F., Valente, W.A. (1984). Monoclonal Antibodies and the Thyrotropin Receptor. In: Greaves, M.F. (eds) Monoclonal Antibodies to Receptors. Receptors and Recognition, vol 17. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-5566-0_9

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  • DOI: https://doi.org/10.1007/978-94-009-5566-0_9

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